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Sakura Cyto-Tek 2500, Operating Manual

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Sakura Cyto-Tek 2500 Operating Manual Download Page 4

 

 

The brand names of products that have been registered or trademarked by  
and are owned by Sakura Finetek USA, Inc., Sakura Finetek Japan Co., Ltd.,  

and Sakura Finetek Europe B.V. appear here: 

 
Cyto-Tek 
Sakura 
Tissue-Tek 

 

 
 

 

 

 

 
 

 

 

 
 

 

 
 

 

© 2015 Sakura Finetek USA, Inc. 

 

All Rights Reserved 

Printed in U.S.A. 

 
 

 

 

 
 

 

 

 

 

 

 
 

 

 

 
 

 

 

 
 

 

 

Revised 21 Dec 2015   

0007129-01 Rev. C 

 

Manufactured for: 
 

Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A. 

 

Sakura Finetek Japan Co., Ltd., Tokyo, 103-0023, Japan 

 

Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, NL 

 

 

 

Summary of Contents for Cyto-Tek 2500

Page 1: ...Cyto Tek 2500 Cytocentrifuge Operating Manual ...

Page 2: ...O p e r a t i n g M a n u a l Cytocentrifuge Cyto Tek 2500 ...

Page 3: ......

Page 4: ... and Sakura Finetek Europe B V appear here Cyto Tek Sakura Tissue Tek 2015 Sakura Finetek USA Inc All Rights Reserved Printed in U S A Revised 21 Dec 2015 0007129 01 Rev C Manufactured for Sakura Finetek USA Inc Torrance CA 90501 U S A Sakura Finetek Japan Co Ltd Tokyo 103 0023 Japan Sakura Finetek Europe B V 2408 AV Alphen aan den Rijn NL ...

Page 5: ...acking 9 2 4 Unpacking the Instrument 9 2 5 Standard Accessories and Options 12 2 5 1 Standard Accessories 12 2 5 2 Options Consumables 12 2 5 3 Missing or Damaged Items 12 3 OPERATING INSTRUCTIONS 3 1 Operating Instructions 13 3 1 1 Workflow Figure 3A 13 3 2 Customization of Settings 13 3 2 1 Creating a Program 13 3 3 Assembling the Specimen Chamber and Dispensing the Specimen 16 3 4 How to Load ...

Page 6: ... Maintenance by a Service Contractor 27 5 TROUBLESHOOTING AND SERVICE 5 1 Power Outage 29 5 2 Actions to be Taken When an Error Occurs 29 5 3 Troubleshooting Charts 30 5 3 1 Error Code of Monitored Errors 31 5 3 2 Other Errors 33 5 4 Service Information 34 5 4 1 When there is a Problem with the Instrument 34 5 4 2 Cyto Tek 2500 Cytocentrifuge PreService Checklist 34 5 4 Accessory Items 35 5 5 Repl...

Page 7: ... automatic paced controlled optimal acceleration The rotor speed is programmable in 10 rpm increments from 200 rpm to 2500 rpm Centrifugation times are programmable from 1 second to 99 minutes 59 seconds The software can store up to 30 programs The ON OFF switch control panel and digital displays are all located on the front of the instrument for easy access The Cyto Tek 2500 was designed with enh...

Page 8: ...owl to transfer rotation from the motor 1 3 2 Guard Bowl Unit Figure 1C 1D The guard bowl unit is comprised of the guard bowl rotor and bowl lid The bowl lid must be put on the guard bowl during handling and operation CAUTION The guard bowl and bowl lid are adjusted unit by unit Avoid using a combination of the guard bowl with the bowl lid of a different unit Guard Bowl Accommodates the rotor The ...

Page 9: ...tation speed or process time Program Number display 7 segment display Displays a program number from 1 to 30 Speed display 7 segment display Displays a programmed rotation speed or an actual speed Time display 7 segment display Displays a programmed process time or a process time left Lid Lock indicator An orange light is displayed when the instrument lid is locked prior to the start of operation ...

Page 10: ... Operating Conditions Operational temperature 10 to 40 C 50 to 104 F Operational relative humidity 30 to 85 RH non condensing Storage temperature 20 to 60 C 4 to 140 F Storage relative humidity 20 90 RH non condensing Noise level 50 dBA or lower when properly balanced Instrument Life Expectancy 7 Years Conditions The safety precautions provided in the operating manual and medical package insert mu...

Page 11: ...9 184 500 29 288 600 42 414 700 58 564 800 75 737 900 95 932 1000 117 1151 1100 142 1393 1200 169 1658 1300 198 1946 1400 230 2256 1500 264 2590 1600 301 2947 1700 339 3327 1800 380 3730 1900 424 4156 2000 470 4605 2100 518 5077 2200 568 5572 2300 621 6090 2400 676 6631 2500 734 7195 NOTES The distance from the center of the rotor to a specimen chamber installed is 105 mm Relative centrifugal forc...

Page 12: ...1 INTRODUCTION Page 8 ...

Page 13: ... servicing equipment with motors cooling units heating units or light sources 5 Mark a 30 cm one foot boundary around the cytocentrifuge and permit no person or hazardous material within this zone while the cytocentrifuge is operating 2 3 Unpacking Open the carton The carton will contain the following accessories Accessories packed in the secondary accessory carton include NOTE An English explanat...

Page 14: ...m block insert Figure 2D Figure 2D 6 Unwrap the proactive vinyl sheet from the instrument and completely open the lid Figure 2E Figure 2E 7 Remove adhesive tape that holds the guard bowl unit in place and close the lid Figure 2F Figure 2F 8 Grasp the bottom of the instrument and carefully remove the instrument from the carton Figure 2G Figure 2G Guard bowl unit Adhesive tape ...

Page 15: ... from the instrument Figure 2I Figure 2H Figure 2I 10 Remove a foam cushion Figure 2J Figure 2J 11 Install the lid gasket see Section 4 1 3 Figure 2K 12 Replace the guard bowl into the instrument Figure 2K Figure 2K Lid open push button Guard bowl unit Foam cushion Lid gasket Guard bowl unit ...

Page 16: ...stomers only If you are located outside the U S contact your nearest Sakura instrument distributor Description Product Code Quantity Power Cord 100 115V A4010548 or Power Cord 230V A4010549 1 Guard Bowl Unit F60 755 00 Guard Bowl Rotor Bowl Lid 1 set 1mL Specimen Chamber Kit 4301 Includes the following items 12 each Specimen Chamber 1mL Chamber Holder 1mL Chamber Cap 1mL Paper Filter 1mL Operating...

Page 17: ... second or more the value will change by 50 3 Press the SET key to save the changes and enter the time minutes setting mode Assemble the specimen chambers dispense specimen into each chamber and install the chamber cap securely CAUTION Use microscopicglassslides withgroundedges CAUTION Glassslides mightbreak dependingontheirsizeandquality Determineif glassslidesyou wanttouse canbesafelyassembled i...

Page 18: ... the Time Display indicates the desired time in seconds 3 Press the SET key to save the changes NOTE To return to the standby mode without saving the change press the START STOP key Set and View Mode When the SET key is held down for 3 seconds or more and released the instrument will start the set view mode for setting system options and viewing the software version number and error logs This set ...

Page 19: ...ound volume levels are available for the completion and error alarms Use the UP and DOWN arrow keys to change the value in the Time display from 1 3 Each setting is as follows 1 Low 2 Middle 3 Loud 2 To test the sound volume press the START STOP key Press the key again to stop the alarm 3 Press the SET key to save the change and switch to the software version number display Viewing the Software Ve...

Page 20: ...6mL and 12mL specimen chambers rubber gaskets 6 12mL specimen chamber holder and cap are reusable after being disinfected and washed However if they have cracks or are deformed do not use them Do not use glass slides that have flaws They will be broken during operation After the specimen has been dispensed immediately lock the bowl lid and start the operation Do not place different types of the ch...

Page 21: ... 1 0 mL of specimen into the specimen chamber Then tightly seal the specimen chamber with a specimen chamber cap Figure 3O Figure 3O NOTE 1 Do not fill the 1 0 mL specimen holder with more than 1 0 mL of specimen as excess specimen may aerosol into the rotor bowl NOTE 2 A better dispersion of cells on the glass slide may be obtained by diluting small volume samples 0 3 mL or less to a final volume...

Page 22: ... a name and ID on each glass slide match those of individual patients or subject CAUTION Load the Cyto Tek 2500 Cytocentrifuge rotor so that it remains balanced An improperly loaded unbalanced rotor will cause the cytocentrifuge to vibrate excessively This can shorten the life of the instrument 1 Open the instrument lid to remove the guard bowl unit 2 Place the guard bowl inside a safety cabinet T...

Page 23: ...ith your fingers to lock the lid Figure 3X Figure 3X CAUTION Make sure that the bowl lid is firmly locked CAUTION If the guard bowl unit needs to be transferred with the specimen loaded chambers set in the rotor be sure to lock the bowl lid and carefully carry the guard bowl with both hands 3 5 Starting the Operation 1 Set the power switch to the ON position 2 Place the guard bowl into the instrum...

Page 24: ... to the standby mode and resets the displayed time to zero 3 7 Emergency Stop To stop the operation immediately switch off the instrument Figure 3AA Figure 3AA 3 8 Removing the Guard Bowl CAUTION In order to minimize a risk of infection place the guard bowl inside a biological safety hood when installing or removing the bowl lid 1 When the operation has ended a buzzer sounds to inform the operator...

Page 25: ...nts used as they are disposable CAUTION Drain the fluid waste accumulated in the guard bowl after every run CAUTION Do not leave the bowl lid locked after use If the pressure inside the guard bowl becomes negative the lid will become difficult to remove ...

Page 26: ...3 OPERATING INSTRUCTIONS Page 22 ...

Page 27: ...area and the well with a clean cloth moistened with neutral detergent Dry any excessively moist areas CAUTION Do not use volatile liquids such as solvents 4 1 2 Cleaning the Instrument Wipe the external surfaces Lid Gasket area and Guard Bowl Storage area with a clean cloth moistened with 70 80 ethyl alcohol or isopropyl alcohol for disinfection Dry any excessively moist areas CAUTION Do not spray...

Page 28: ... 4E The guard bowl unit has been disassembled as shown below Figure 4F Figure 4F Assembly of the Guard Bowl Unit 1 Install the V shape gasket Figure 4G and 4H Figure 4G Figure 4H CAUTION Install the V shape gasket on the bowl shaft housing with the projecting part of the gasket facing upward Failure to do so may weaken the seal capacity Bowl Lid Guard Bowl Rotor Bowl Lid Gasket V shape V shape gas...

Page 29: ...jecting part of the gasket to the back side of the groove as illustrated in the above section view 4 Determine two points a and b so that the gasket is in exact halves Place the point a at the top of the gasket groove and the point b at the bottom and fit two points of the gasket into the groove Next determine two points c and d so that the upper and lower lengths of the gasket become the same Fit...

Page 30: ...rite solution common bleach are the recommended disinfectants 2 After soaking the components in an approved disinfectant they should be cleaned in hot water and Component Disinfection Sterilization Bowl Lid Can be sterilized in an autoclave 121 C 20 minutes Can be disinfected by soaking in ethyl alcohol 80 isopropyl alcohol 70 or sodium hypochlorite solution 0 5 or less in concentration for less t...

Page 31: ...akura Technical Support Department at 1 800 725 8723 option 2 If located outside the United States contact the nearest Sakura instrument distributor or representative for information and assistance 4 1 5 Gasket Replacement Replace the bowl lid gasket and V shape gasket when they get deteriorated or at least once a year When installing replacement gasket note the correct orientation of the gasket 4...

Page 32: ... 28 Figure 4N 3 Put a small amount of grease on the spindle in eight places as shown below 4 Use a dry paper towel such as Kimwipe to spread the grease evenly over the circumference of the protruding rims on the side of the spindle ...

Page 33: ...ss and more than 5cm long WARNING BEFORE OPENING THE INSTRUMENT LID AFTER IT IS UNLOCKED TAKE INFECTION CONTROL MEASURES SUCH AS WEARING PROTECTIVE CLOTHING AND PLACING THE INSTRUMENT IN A SAFETY CABINET 5 2 Actions to be Taken When an Error Occurs If an error occurs during operation the instrument immediately decelerates the motor and sounds the alarm After the motor has stopped the lid lock indi...

Page 34: ...harts list caution messages and error codes that could occur during operation of the Cyto Tek 2500 Cytocentrifuge Possible problems could be electrical operational or mechanical Probable causes and recommended remedies are also included so that isolated problems can be quickly corrected If additional assistance is required concerning an instrument problem an error code displays that is not listed ...

Page 35: ...Poorconnector contact Controllerboardfailure Lidlockdetection sensor failure 87 Theinstrumentlidisopenedduring operation Faultylid sensoror improper wiring ContactSakuraTechnicalSupport ordistributor Poorconnector contact Controllerboardfailure Lidlockdetection sensor failure 88 Thelockwasreleased during operation Faultysolenoid orwiring ContactSakuraTechnicalSupport ordistributor Poorconnector co...

Page 36: ...rguard bowl placement Placetheguard bowlproperly Poorconnector contact Placetheguard bowlproperly ContactSakura TechnicalSupportordistributor Controllerboardfailure Faultymotor motordriveror wiring 97 Motorerror themotor salarmfunctionwas triggered Temperatureerror Turntheinstrument poweroff Operate the instrument again in30 minutes Ifthe sameerror occurs contact Sakura TechnicalSupport or distrib...

Page 37: ...e is normal Faulty power cord Confirm that the power cord is firmly plugged Contact Sakura Technical Support or distributor Switching power supply failure Leakage in switching power supply Blown fuse s An electric shock occurred The grounding wire to the power input is disconnected Make sure that grounding wire is firmly connected Leakage in the power input area Contact Sakura Technical Support or...

Page 38: ... be solved and an instrument failure is apparent our Technical Support Department is available to assist you Before calling for instrument service collect the information requested in the PreService Checklist of this section This information will help the Technical Support Representative identify the probable cause of the instrument malfunction The instrument should be disinfected prior to being s...

Page 39: ...0 Bowl Unit F60 97 00 Rotor Unit A3 400 048 Time Delay Fuse HT 2AN5 B3 01 0000 V Ring 0007129 01 Operating Manual D9 60 003A Grease 10 g North America F61 058 00 Grease 10 g other than North America 5 6 Where to Order If located within the United States Accessory Items and Replacement Parts are available directly from Order Management Sakura Finetek USA Inc 1750 West 214th Street Torrance CA 90501...

Page 40: ...5 TROUBLESHOOTING AND SERVICE Page 36 ...

Page 41: ...in death or serious injury to the operator and or other personnel Warning indicates a potential hazard in which failure to follow instructions may result in serious injury to the operator and or other personnel Caution indicates a potential hazard in which failure to follow instructions may result in injury to the operator and or other personnel or damage to the instrument and or other property No...

Page 42: ...ugging the instrument can also turn off the main power supply If the instrument malfunctions remove the power plug from the power receptacle At instrument installation choose such a place as a wall outlet is not hidden behind the instrument so that you can quickly unplug the instrument in the event of emergency Do not connect to a piggybacked electric outlet Do not use an extension cord Doing so m...

Page 43: ...r before performing cleaning unless otherwise instructed in this manual After sterilized by an autoclave the guard bowl and other components will be very hot Be careful not to get burned Use specified fuses Place the instrument on a sturdy laboratory table or bench Failure to do so may result injury or accident Install the instrument in a horizontal and vertical posture The instrument in a slanted...

Page 44: ...D on each glass slide match those of individual patients or subjects Do not leave the guard bowl lid locked after use The bowl lid left locked after use may become difficult to remove if pressure inside the guard bowl becomes negative Do not put an obstruction on movable components Doing so may cause instrument failure Store the instrument with the power cord unplugged for extended non use If the ...

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