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3.
Insert the two tabs at the bottom of a clean
specimen chamber into the corresponding holes of
the chamber holder, as shown below (Figure 3M).
Figure 3M
4.
Align the square hole of the paper filter with the
hole in the specimen chamber holder. Press the
top of the specimen chamber back toward the
specimen chamber holder, flatting it out, until it
engages the restraining latch on the top of the
specimen chamber holder (Figure 3N).
Figure 3N
5.
Prepare other specimen chamber assemblies.
6.
Hold the specimen chamber holder vertically, so
that the graduations of 0.5 mL and 1.0 mL, marked
on the cylindrical portion of the specimen chamber
are clearly visible. Dispense from 0.1 mL to 1.0 mL
of specimen into the specimen chamber. Then
tightly seal the specimen chamber with a
specimen chamber cap (Figure 3O).
Figure 3O
NOTE 1:
Do not fill the 1.0 mL specimen holder with more
than 1.0 mL of specimen, as excess specimen
may aerosol into the rotor bowl.
NOTE 2:
A better dispersion of cells on the glass slide
may be obtained by diluting small volume
samples (0.3 mL or less) to a final volume of 0.5
mL with an appropriate diluent.
Handling of 6mL and 12mL Specimen
Chambers
1.
Write the name of the specimen on a clean glass
slide and place the slide, frosted side up, in a
specimen chamber holder.
2.
Place the 6mL rubber gasket on the glass slide to
use for the 6mL specimen chamber. Similarly, to
use for the 12mL specimen chamber, place the
12mL rubber gasket on the glass slide (Figure 3P).
Figure 3P
Chamber cap
1.0mL
0.5mL
6mL rubber gasket
The illustration is the 6mL chamber.
