26
Galaxy S+ CO
2
Incubator
User Guide
4
4
R
R
O
O
U
U
T
T
I
I
N
N
E
E
M
M
A
A
I
I
N
N
T
T
E
E
N
N
A
A
N
N
C
C
E
E
4.1
General Notes
To ensure that chamber conditions remain as stable as possible, be sure to minimise
the length of time that the door is open. The magnetic door catches are specifically
designed to make door opening and closing as easy as possible. When you open
the door, wipe off any small drops of condensate that may have formed on its seal.
This will avoid a build-up of condensation.
If you are using the humidity tray for humidification, be sure to follow the indications
provided
in Section 3.3.
4.2
Daily Checks
1.
Check that the temperature and CO
2
levels are reading within specification.
2.
Check the reserve pressure in the CO
2
cylinder (normally 725psi or 50 bar when
full). The design of the incubator ensures very low consumption of CO
2
: during
normal working conditions, a typical large cylinder should last approximately 12
months. If there is a significant drop at the cylinder pressure of 725psi or 50 bar,
it means that the cylinder is almost empty and should be replaced. Ensuring that
there are no leaks at any of the connections will ensure a greater lifetime to the
CO
2
supply and will help avoid accidentally running out of CO
2
.
4.3
Weekly Checks
Refill the humidity tray (to a maximum volume of 2.5 litres). The use of warm water
(
∼
37.0
°
C) will ensure a rapid return to optimum chamber conditions.
4.4
Monthly Checks
If required, you can take a sample of the gas inside the chamber using the CO
2
sample port, and check it using a CO
2
gas analyser. The CO
2
sample port is located
on the right hand side of the equipment tray (
see Figure 7
).
If you conduct a sampling, please ensure the following:
Turn off the CO
2
gas by re-programming the set point for CO
2
to 0.0% to
prevent CO
2
from being injected into the chamber and giving a false reading.
A flow rate
≤
0.5 litres/ minute is used to take a sample.
Remember to re-set the CO
2
set point to the desired level after sampling.