Carbon Dioxide ISE Probe
(PS-3517)
Introduction
The Carbon Dioxide Ion Selective Electrode (ISE) Probe is a
gas-sensing electrode that allows fast, simple, economical, and
accurate measurement of dissolved CO
2
in aqueous solutions.
Components
Internal glass electrode
The sensing element of the ISE Probe.
BNC connector
Use this to connect the probe to a pH sensor, such as the
Wireless pH Sensor (PS-3204), via the included BNC
cable.
Membrane module
Holds the Internal Fill Solution which is used in the
assembled electrode.
Storage bottle
Contains storage solution used to preserve the functionality
of the electrode while not in use. See Electrode storage
for more information on storing the electrode.
Included equipment and solutions:
• Carbon Dioxide Electrode
• Storage bottle
• Membrane module (2x)
• BNC male-to-BNC male cable
• Internal Fill Solution
• 1000 ppm Carbon Dioxide Standard Solution
• Ionic Strength Adjuster (ISA)
• Pipette
Required equipment and solutions:
• Wireless pH Sensor (PS-3204)
NOTE: You can also use the PASPORT High
Precision pH/Temperature with ISE/ORP
Amplifier (PS-2147) and a compatible
PASPORT interface. However, unlike the
Wireless pH Sensor, the PASPORT
pH/Temperature sensor does not have direct
concentration reading capabilities.
• PASCO Capstone or SPARKvue data collection software
• Wash bottle filled with distilled or de-ionized (DI) water
• pH 4.0 Buffer Solution
• pH 7.0 Buffer Solution
• Lab wipes
• Clean volumetric flasks (100 mL)
• Clean beakers
Get the software
You can use the Carbon Dioxide ISE Probe with SPARKvue or
PASCO Capstone software. If you’re not sure which to use, visit
pasco.com/products/guides/software-comparison
SPARKvue is available as a free app for Chromebook, iOS, and
Android devices. We offer a free trial of SPARKvue and
Capstone for Windows and Mac. To get the software, go to
or search for SPARKvue in your device’s
app store.
If you have installed the software previously, check that you have
the latest update:
SPARKvue
Go to Main Menu
> Check for Updates
PASCO Capstone
Go to Help > Check for Updates.
Using Ionic Strength Adjuster (ISA) solution
to improve accuracy
At low concentrations of CO
2
ions, a standard method for
removing the influence of charged particles on the detector is to
add Ionic Strength Adjuster (ISA) solution to each of your
standard solutions and samples. The addition assures that the
overall ion activity in each solution being measured is nearly
equal. The ISA contains no ions common to the Carbon Dioxide
ISE itself.
Electrode assembly
IMPORTANT! Please read these instructions before
assembling the Carbon Dioxide ISE Probe!
1. Carefully remove the internal glass electrode from the
storage bottle. Set the storage bottle aside for later use.
NOTE: Soaking the internal glass electrode in
Internal Fill Solution or pH 4.0 buffer for at least
1 hour before proceeding with the following
steps will ensure fast response.
2. Fill one of the membrane modules with approximately
0.75 mL of the provided Internal Fill Solution.
3. Rinse the internal glass electrode with DI water and blot
dry. Place the electrode into the membrane module filled
with Internal Fill Solution and tighten the cap until snug. Do
not over-tighten.
Product Guide | 012-15719B
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