Rotor-Gene Q MDx CE User Manual 02/2022
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Remove/Remove All
:
Remove
removes the highlighted channel.
Remove All
removes all channels.
Start
:
Start
begins Gain Optimisation. A gain is chosen which results in fluorescence signal
levels within the specified range. If fluorescence falls outside the specified range, the
gain is set to give the closest match possible.
Manual
:
This opens the
Manual Gain Adjustment
window.
Changing Gain During a Run
:
If the gain at the beginning of the run was too high or too low, it can be changed
within the first ten cycles. A vertical line appears where the gain has been changed.
The cycles before the change are excluded from the analysis.
Note
: Gain Optimisation may choose a setting which does not fall within the specified range. This
can be due to changes in fluorescence after the first Hold step. However, the result of Gain
Optimisation provides a good indication of the fluorescence level the run will be started on.
Manual Gain Adjustment
To perform “Manual Gain Adjustment”, click on
Manual…
in the
Auto-Gain Optimisation Setup
window. The
Manual Gain Adjustment
window appears. This window displays the fluorescent
readings at any given temperature in real-time. It is used when the background of a sample is
unknown and therefore the gain must be determined to ensure the sample signal is sufficient for
detection.