Rotor-Gene Q MDx CE User Manual 02/2022
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“Spline Curve Fit” assumes only that concentration values increase with fluorescence. While this
approach makes estimates of nonlinear data more accurate, it cannot provide ANOVA, as it does
not assume a linear model.
6.6.11
High Resolution Melt analysis
High resolution melt (HRM) analysis characterizes samples based on sequence length, GC content,
and complementarity. HRM analysis is used in genotyping applications, such as analysis of gene
mutations or single nucleotide polymorphisms (SNPs), and in epigenetics applications for analysis
of DNA methylation status. HRM analysis provides accurate results and savings on probe and label
costs compared to other methods.
To perform the analysis, select
Other
and then
High Resolution Melt Analysis
in the
Analysis
window. Double-click on the channel to analyze. The melt curves from the raw channel are
normalized by averaging all starting and ending fluorescence values and then forcing the end
points of each sample to be the same as the average.
Autocalling of samples is achieved by clicking
Genotypes
. Enter the name of the genotype, followed
by the sample number, which is used as a positive control to call unknown samples automatically.