Qiagen Rotor-Gene Q MDx CE, User Manual, Page: 4 / 208

Rotor-Gene Q MDx CE User Manual 02/2022 4
5.2.2 Reaction setup ......................................................................................70
5.2.3 Rotor-Disc setup ....................................................................................73
6 Analysis User Interface ............................................................................................77
6.1 Workspace ..................................................................................................77
6.2 Toolbar .......................................................................................................77
6.3 View raw channels .......................................................................................77
6.4 Toggling samples .........................................................................................78
6.5 File menu .....................................................................................................80
6.5.1 New ...................................................................................................80
6.5.2 Open and Save ....................................................................................81
6.5.3 Reports ................................................................................................82
6.5.4 Setup ..................................................................................................83
6.6 Analysis menu ..............................................................................................83
6.6.1 Analysis ..............................................................................................83
6.6.2 Quantitation.........................................................................................85
6.6.3 Two standard curve ..............................................................................96
6.6.4 Delta delta C
T
relative quantitation ........................................................100
6.6.5 Melt curve analysis .............................................................................103
6.6.6 Comparative quantitation ....................................................................106
6.6.7 Allelic discrimination ...........................................................................108
6.6.8 Scatter graph analysis .........................................................................109
6.6.9 EndPoint analysis ................................................................................111
6.6.10 Concentration analysis ........................................................................117
6.6.11 High Resolution Melt analysis ...............................................................120
6.7 Run menu ..................................................................................................121
6.7.1 Start Run ............................................................................................121
6.7.2 Pause Run ..........................................................................................121
6.7.3 Stop Run ............................................................................................122
6.8 View menu ................................................................................................122
6.8.1 Run Settings .......................................................................................122
6.8.2 Temperature Graph ............................................................................125