Qiagen Rotor-Gene Q MDx CE, User Manual, Page: 190 / 208

Rotor-Gene Q MDx CE User Manual 02/2022 190
12 Glossary
Term Description
Acquisition Acquisition is the collection of fluorescent data. Each acquisition (set of fluorescent data)
from a channel is displayed in the software as unanalyzed data in a “Raw channel”
window. This data can be analyzed using the options in the “Analysis” menu.
Bins In a melt analysis, bins are set to define a region where a melt peak is expected to occur.
Genotypes can be defined based on the presence of peaks in certain bins or combinations
of bins.
CE-IVD Compliance with the in vitro diagnostic medical device European Directive 98/79/EC.
Channel A channel consists of a light emitting diode (LED) with an excitation filter paired with an
emission filter. The LED and excitation filter excite samples at a given wavelength.
Fluorescence emitted by samples is passed through the emission filter, before being detected
by a photomultiplier.
Gain The Rotor-Gene Q MDx uses a photomultiplier to collect fluorescence photons and convert
them to electronic signals. The gain is a setting that determines the sensitivity of the
photomultiplier. If the gain is set too high, the signal is oversaturated. If the gain is set too
low, it is not possible to differentiate signal from background noise.
Gain Optimisation Gain Optimisation is a process that dynamically adjusts the gain setting, allowing an
appropriate setting to be selected which results in optimal signal detection.
Loading Block Loading Blocks are aluminum blocks available in different formats which are used to hold
tubes or Rotor-Discs during reaction setup. Rotor-Disc Loading Blocks are also used with the
Rotor-Disc Heat Sealer to heat seal Rotor-Discs.
Locking Ring Locking Rings are metal rings that fit onto the rotor to prevent tubes and caps from coming
loose during operation of the Rotor-Gene Q MDx. Loose caps and tubes could cause
damage to the instrument.
Rotor The metal rotor holds tubes or Rotor-Discs in the Rotor-Gene Q MDx. It enables samples to
spin in the instrument chamber and ensures that samples are correctly aligned with the
optical system. The rotor is secured with a Locking Ring.
Rotor-Disc Rotor-Discs are circular plates of vertically oriented reaction wells. Rotor-Disc formats for 72
and 100 reactions are available. Rotor-Discs are sealed using Rotor-Disc Heat Sealing Film
and the Rotor-Disc Heat Sealer.