Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
13
www.promega.com
TM276 · Revised 5/21
6.
The GloMax
®
20/20 Luminometer will automatically turn off.
7.
Turn on the luminometer with the module properly installed. The controls specific to the Fluorescent Module
will automatically appear on the touch screen.
8.
Subsequent installation of the Fluorescent Module will automatically launch the proper touch screen controls.
9.B. Touch Screen Controls
Home Screen:
From the Home Screen you can access
Help
,
Tools
,
Calibration
,
Mode
or
Measure Fluorescence
.
Touch the
Help
button to access a variety of help topics.
Mode:
There are two measurement modes for the Fluorescent Module. The Raw Fluorescent mode provides a simple
method to measure the fluorescence of a sample without calibration. The Direct Fluorescent mode requires a calibration
with one standard and one blank. The Direct Fluorescent mode reports readings in one of several units (i.e., µg/ml,
ng/ml, pg/ml, ppb, ppt or FSU). With a valid calibration, it is not necessary to generate a standard curve to determine
the actual concentration of the sample.
Calibration:
Touch
Calibration
to calibrate the Fluorescent Module with a known standard. Select the units of
measurement that correspond to the units of your standard. For example, if your standard is 200ng DNA/ml, select the
ng/ml option. Next, enter the final concentration of your standard. We recommend selecting a standard with a
concentration similar to a typical sample concentration. Subsequent sample readings reflect the actual concentration.
Measurement:
Analysis with the Fluorescent Module requires the minicell cuvette. The maximum volume of the
minicell is 250µl. The minimum volume is 100µl. Insert the minicell cuvette into the Fluorescent Module. Close the lid
and touch the green
Measure Fluorescence
button on the touch screen. After a 5-second measurement time, the
result will appear on the touch screen next to the Sample ID.
9.C. Removing the Fluorescent Module
1.
Turn off the GloMax
®
20/20 Luminometer.
2.
Unlock the module by pulling up on the red knob. The module will disengage from the luminometer.
3.
Carefully remove the Fluorescent Module and store it in the product case
.
