Planer Kryo360-1.7 Operator'S Manual Download Page 42

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Liquidus Tracker

3.2.2.1.1 Dual solution mode

The basic flow system is simplified as shown in the diagram below.

1. DMSO solution Ksol1%

2. Peristaltic pump 1 running at F1 mL/min/rpm

3. 0% solution

4. Peristaltic pump 2 running at F2 mL/min/rpm

5. Y piece

6. Sample container

7. Waste collection container

8. Filtered exhaust vent

9. Filtered inlet vents

10. Injection line 1

11. Injection line 2

12. Combined injection line

13. Extraction line

14. Pressure relief valve.

15. Boundary of the chamber

In this system, two solutions are pumped by two peristaltic pumps into the sample container. The

Summary of Contents for Kryo360-1.7

Page 1: ...Liquidus Tracker Operator s Guide...

Page 2: ...e you with copies You may freely copy and distribute this document in any form provided that the document is reproduced in full and includes these notices Individual pages and forms may be freely copi...

Page 3: ...talling the system 18 18 1 Installing a Planer pump and dewar 18 Connecting the delivery hose 18 Filling the dewar 19 Fitting the pump 19 Testing the dewar 20 Removing the pump 21 2 Installing a CryoC...

Page 4: ...to a USB flash drive Section IV Routine maintenance and troubleshooting 62 62 1 Cleaning the system 62 2 Troubleshooting 63 No slot option when downloading a profile 63 No slot option when retrieving...

Page 5: ...Introduction Section I...

Page 6: ...1 1 Symbols 1 1 1 Symbols used in this manual This shows information or instructions that are related to safety Failure to follow these instructions may result in personal or third party injury This s...

Page 7: ...g to a computer running DeltaT RS232 connection to the peristaltic pumps Connection for USB flash drive Ethernet connection Alarm output connector RST Reset button This button will reset the controlle...

Page 8: ...arning Failure to comply with these instructions could result in serious injury Operating the system in a manner not specified within in this manual or under conditions outside of the specifications m...

Page 9: ...nitrogen Only use vessels designed for working with liquid nitrogen Secure the liquid nitrogen container to prevent toppling Ensure that the delivery pipe connections are secure and leak free before s...

Page 10: ...conds or less to avoid overheating Do not move the chamber whilst still wet after a freezing run The liquidus tracker requires significant volumes of cryoprotectants such as Dimethyl Sulfoxide DMSO to...

Page 11: ...t may be unfamiliar to you These terms are explained below Profile A profile defines the temperature time path that the chamber should follow The profile comprises a number of steps each of which can...

Page 12: ...ides time for all the samples to equilibrate at the seeding temperature The controller then prompts the user that the samples are ready for seeding The user would normally momentarily remove the sampl...

Page 13: ...Run from panel select profile this would indicate that you should select the Run from panel from the main menu and then select the appropriate profile 1 6 About the system The Liquidus Tracker is a sy...

Page 14: ...LED Indicates that the system is applying the heater to control the profile 3 Information LED Indicates that the system requires attention 4 Main display 5 Run Enter key Used to start a run Also used...

Page 15: ...olled from right to left In some cases there may be multiple selections to display The main menu is an example where there are a number of items that need to be presented When this occurs only one ite...

Page 16: ...12 Liquidus Tracker 1 6 2 The connections The connections to the controller are all made along the underside of the main electronics enclosure...

Page 17: ...360 chamber provides power directly to the controller and for these systems the power supply is not required 4 USB connection for communication to the PC 5 RS232 connection for driving the peristaltic...

Page 18: ...ide of the controller Note Do not operate the manual override buttons during normal use as they will affect the temperature control 1 Manual override cool 2 Manual override heat 3 PRG Programming butt...

Page 19: ...nning during a power failure This backup can support the controller for up to 1 minute during which time the control algorithms will continue to function If power is restored during this period the co...

Page 20: ......

Page 21: ...Installing the system Section II...

Page 22: ...e female ferrule at the other end of the hose to the outlet on the side of the pump Ensure that the fibre washer is fitted to this joint Tighten securely with a spanner 2 1 2 Filling the dewar Warning...

Page 23: ...operates on the neck ring It is normal for nitrogen gas to issue from the pressure relief valve 3 Press firmly on the top of the pump to hold it level and clip all the springs onto the dewar alternati...

Page 24: ...wall socket 3 Open the red pressure relief valve Cold gas will issue from the valve 4 Wait until the pressure on the gauge has fallen to zero and the flow of gas has stopped 5 Disconnect the pump fro...

Page 25: ...the hose is not already fitted to the Chamber wrap the male hose thread with PTFE tap and fix to the N2 input on the back of the chamber Tighten securely with a spanner 2 Connect an appropriate adapte...

Page 26: ...ontroller Refer to the diagram in the Connections section when following the instructions below Items in the diagram are shown as n in the text 1 Remove the two screws A and B retaining the rear cover...

Page 27: ...23 Installing the system 2 For Kryo360 1 7 chambers connect the 15 way D Type socket from the chamber to the chamber adapter connection cable...

Page 28: ...to 37 way adapter cable 4 Kryo360 1 7 chambers can provide power directly to the controller For these chambers connect the DIN plug connector from the chamber adapter cable to the DC power inlet 6 on...

Page 29: ...25 Installing the system...

Page 30: ...r For these chambers connect the 37 Way to 15 Way D Type adapter cable between the chamber cable and the chamber connector 2 on the controller Connect the DIN connector from the separate power supply...

Page 31: ...ystem has been provided with a stirring lid the cable for the pumps will be fitted with an additional 3 way DIN plug for connection to the stirring controller In this case connect the stirring control...

Page 32: ...28 Liquidus Tracker 8 Connect the USB connector to a spare USB port on the PC 9 Refit the rear cover plate to the controller...

Page 33: ...29 Installing the system 10 Connect the power supply cables to the pumps and chamber...

Page 34: ...arning The mains power outlet is the main disconnect device for the power supply Ensure that the power supply cable used is no longer than 3m and that the power outlet is easily accessible Warning Pos...

Page 35: ...Operating the system Section III...

Page 36: ...time you use the system o Create a profile Run a profile 3 1 Switching on the system Note For Kryo 360 chambers the controller derives its power from the main chamber 1 Switch on the chamber using the...

Page 37: ...the system will automatically present you with the clock screen every time the system is switched on This means that you can continue to use the system but you should contact your distributor to get t...

Page 38: ...will need to configure the system so that it can determine the relationship between the pump speeds and the current chamber temperature 1 Switch on the Liquidus Tracker 2 When the power up cycle has c...

Page 39: ...35 Operating the system 6 The main Coefficient control screen will then be displayed 7 Select the Liquidus Tracker option...

Page 40: ...ows a indicating successful transmission Tip It is possible to check the coefficients in the controller by clicking the Read button However some values are derived from the controller s stored paramet...

Page 41: ...extract the extra solution and maintain a constant volume of cryoprotectant around the sample The meaning of some parameters will change depending on the selected operating mode As such you must selec...

Page 42: ...ution 4 Peristaltic pump 2 running at F2 mL min rpm 5 Y piece 6 Sample container 7 Waste collection container 8 Filtered exhaust vent 9 Filtered inlet vents 10 Injection line 1 11 Injection line 2 12...

Page 43: ...ate for each pump in mL rpm Flow rate Fn rpm Offn Ksol1 is the concentration of the solution delivered by pump 1 The solution provided by pump 2 is assumed to have a concentration of 0 Setting F1 F2 o...

Page 44: ...ltered exhaust vent 9 Filtered inlet vent 10 Injection line 11 Extraction line 12 Pressure relief valve 13 Boundary of the chamber In this system one solution is pumped by a peristaltic pump into the...

Page 45: ...ntainer at the start of the run F1 F2 Off1 and Off2 are the pump flow factors that define the flow rate for each pump in mL rpm Flow rate Fn rpm Offn Ksol1 is the concentration of the solution deliver...

Page 46: ...and Single solution systems see Target temperature The target temperature is used to determine the target concentration as described below The Tn Kn parameters define the temperature concentration cu...

Page 47: ...temperature when calculating the pump drives If TFiltered is the filtered temperature and Tc is the current demand temperature then on each cycle TFiltered Two x TF TWn x Tc Two TWn For the Dual solut...

Page 48: ...e when prompted You will then be given the opportunity to select a run slot from 1 to 10 in which to store the profile The profile will then be stored into the selected slot of the controller and the...

Page 49: ...ystem 3 3 1 Dual solution carrier The diagram below show the configuration of the dual solution carrier system Refer to this diagram when following the instructions below Items in the diagram are show...

Page 50: ...11 Injection line 2 12 Combined injection line 13 Extraction line 14 Pressure relief valve Warning Ensure that a pressure relief valve has been fitted between outlet of pump 1 2 and the waste collect...

Page 51: ...ectant 1 Unless a collapsible container such as a flexible bag is used this container will need to be fitted with an inlet filter 9a to maintain sterile conditions in use 6 Connect the injection line...

Page 52: ...48 Liquidus Tracker 9 Fit the linear carrier to the main chamber lid 10 Fit the injection line 1 10 to pump 1 and the injection line 2 11 to pump 2...

Page 53: ...his diagram when following the instructions below Items in the diagram are shown as n in the text 1 DMSO solution Concentration must match the configuration setting 2 Peristaltic pump 1 3 Peristaltic...

Page 54: ...leak of fluid The pressure relief valve should be set to less than the rated tubing pressure and no more than 1 24 bar 18 psi Note The following steps should be undertaken using aseptic technique 1 C...

Page 55: ...ondyle to the condyle carrier plate using sterile sutures 5 Place the condyle carrier plate into the condyle pot being careful to feed the injection tube in the base of the pot through the hole in the...

Page 56: ...o displace the solution or more cryoprotectant will need to be added and the Liquidus tracking parameter readjusted 11 Screw the lid of the pot onto the carrier 12 Connect the injection line 10 to the...

Page 57: ...ontainer into the main stirring lid Rotate the shaft of the stirrer if necessary to get the magnetic coupling to align 17 Push the three lines from the container pressure relief line 7 injection line...

Page 58: ...using the Liquidus Tracker The first way is to run the profile directly from a PC running DeltaT Further information is given in Running from DeltaT The second method is to run a profile that has bee...

Page 59: ...t to run a profile from the PC leave the system in the PC mode From DeltaT run a profile as normal Tip If you want to retain the profile in the controller s memory so that it can be run later locally...

Page 60: ...the Going to start temperature section 3 4 3 1 Going to start temperature The profile will have been programmed with a start temperature at which the run should begin When a run is initiated the contr...

Page 61: ...l seeding was selected the controller when prompt you at the end of the soak period Chamber 7 0 C Sample 7 0 C 20 Ready to seed Press Enter when done When you have completed seeding the samples press...

Page 62: ...the required profile the information LED will flash and the internal sounder will produce a continuous alarm tone In addition the alarm output contacts will open These may be used to trigger an extern...

Page 63: ...From DeltaT use the Retrieve last run to recall the run data Refer to the documentation provided with DeltaT for more details 3 6 2 Writing the last run to a USB flash drive 1 From the main menu use...

Page 64: ...us Tracker The pdf file comprises two pages The first page shows a graph of the profile chamber and sample temperatures and the required concentration The second page shows the actual profile used for...

Page 65: ...Routine maintenance and troubleshooting Section IV...

Page 66: ...dry fully before reapplying power 2 Power supplies should be wiped clean with a damp cloth Ensure that water is not allowed to enter the supply Leave to dry fully before reapplying power 3 Check the...

Page 67: ...controller fails to respond to any key presses then the system will need to be reset This can be done by pressing the RST button on the side of the controller see Manual override and reset buttons To...

Page 68: ......

Page 69: ...Additional information Section V...

Page 70: ...nsing Operating environment For indoor use only Operating temperature 5 C to 40 C Operating humidity 5 to 90 relative humidity non condensing Altitude up to 2000 m Pollution degree Pollution degree 2...

Page 71: ...15 V 10 50 60Hz 10 A 115 V 10 50 60Hz 15 A 230 V 10 50 60Hz 5 A 230 V 10 50 60Hz 10 A Note The Liquidus Tracking system is designed to be connected to an Installation Category over voltage category II...

Page 72: ...0 V model F 10A L 250V Planer FL011913 F 6A L 250V Planer FL011912 T 3A L 250V Planer FL011909 5 1 5 Batteries Warning This product contains a lithium battery which is an explosion hazard if short cir...

Page 73: ...y be open during the backup period see Power backup As such it is advisable to connect the alarm output to equipment that can latch the alarm state 5 2 Decontaminating the system In most situations ch...

Page 74: ...individual concerned In the case of maintenance or repair of laboratory equipment current DHSS recommendations support the issue of a certificate statement of decontamination prior to submitting the e...

Page 75: ...ransportation B 1 Has this equipment item been exposed internally or externally to hazardous materials as indicated below Provide further details here YES NO Blood body fluids respired gases pathologi...

Page 76: ......

Page 77: ...ler 22 delivery hose 18 connectors location 12 system 12 contamination declaration of contamination status 71 controller connecting 22 specification 66 creating profile 44 cylinder installation 21 D d...

Page 78: ...the system 32 P parameters liquidus tracker 36 temperature concentration table 42 PC mode 55 power backup 15 power up cycle 32 precautions EMC 6 preparation system 33 profile 56 creation 44 definitio...

Page 79: ...special terms dry shipper 7 hold 7 kryo file 7 profile 7 ramp 7 seeding 7 specification chamber 67 controller 66 system 66 start temperature 56 switching on 32 symbols 2 equipment 3 system about 9 cl...

Page 80: ...MA101894 Issue 2 Liquidus Tracker Operator s Guide...

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