Planer Kryo360-1.7, Operator'S Manual

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8 Liquidus Tracker
Seeding
Tip
Seeding is not appropriate for liquidus tracking. The following description is provided for
completeness but should not be used for a liquidus tracking run.
Within a profile, seeding is a special part of the program designed to trigger the onset of freezing
within the sample being frozen. The Liquidus Tracker supports a type of seeding known as manual
seeding. Manual seeding comprises a seeding temperature and a soak time. When the chamber
reaches the seeding temperature, the controller maintains the temperature for a period defined by
the soak time. This provides time for all the samples to equilibrate at the seeding temperature. The
controller then prompts the user that the samples are ready for seeding. The user would normally
momentarily remove the samples and manually trigger the onset of freezing by touching the straws
with a cold implement; the exact method of seeding is defined by the users' protocols and is outside
the scope of this manual. Once the seeding process has been completed, the user would press a
key on the controller to indicate that seeding was complete and that the freezing profile should
continue as normal. The manual seeding process is summarised in the diagram below.
Profile w ith m an u al s eedin g
Although the controller can support profiles with seeding enabled, this feature is not normally used
with liquidus tracking.