23
Connecting N
2
/O
2
gas cylinder
WARNING
Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are connected
correctly and are not liable to become disconnected. Ensure that the gas pressure is set at the
specified value. Improper connection of the gas pipe or use of incorrect gas pressure may result
in leakage of gas.
Elevated level of gas can be hazardous to health and may lead to asphyxiation
and risk of death.
This incubator needs N
2
/O
2
gas depending on the setting of O
2
density. The selection is as follows:
When the setting of O
2
density is less than 18 %: N
2
gas
When the setting of O
2
density is more than 22 %: O
2
gas
O
2
density in the atmosphere is about 20 %. For the control of O
2
density in the chamber, O
2
gas is diluted
by N
2
gas when the setting of O
2
density is less than that of the atmosphere. On the contrary, O
2
gas is
added when the setting of O
2
density is more than that of the atmosphere.
1.
Get 2 N
2
gas cylinders (or 2 O
2
gas cylinders) ready and install optional gas regulators MCO-010R in
each.
Note:
When MCO-010R is not available, install a gas regulator rated at 25 MPa(G) (250 kgf/cm
2
(G), 3600
psi(G)) for the primary side, and 0.25 MPa(G) (2.5 kgf/cm
2
(G), 36 psi(G)) for the secondary side.
2.
Using a gas tube that is provided, connect the
connecting port A for N
2
/O
2
gas pipe and the gas
regulator of the N
2
gas cylinder A (or O
2
gas
cylinder A).
3.
Using a gas tube that is provided, connect the
connecting port B for N
2
/O
2
gas pipe and the gas
regulator of the N
2
gas cylinder B (or O
2
gas
cylinder B).
Note:
·
The gas of connected 2 gas cylinders must be the same one. It is not possible to use an N
2
gas cylinder
and an O
2
gas cylinder simultaneously.
·
By using 2 tube bands that is provided, connect the gas tube tightly to prevent it from coming off.
·
Make sure that the gas tube is not folded.
4.
After connecting the gas tube, make sure that no gas is leaking (ex. by using a gas leak detection
spray).
5.
Set the N
2
gas (or O
2
gas) on the secondary side to 0.05 MPa(G)~0.1 MPa(G) (0.5 kgf/cm
2
(G)~1
kgf/cm
2
(G), 7.3 psi(G)~14.5 psi(G)) for gas injection. Recommended pressure: 0.05 MPa(G) (0.5
kgf/cm
2
(G), 7.3 psi(G)).
Note:
As the pressure increases, the O
2
gas density control range will increase. Excessive pressure may
cause gas supply lines inside the incubator to come loose, which may result in fire or oxygen deprivation
due to gas leak. If gas lines come loose, the incubator must be repaired.
Connecting port A for N
2
/O
2
gas pipe
Lower right side
Connecting port B for N
2
/O
2
gas pipe
