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Phcbi MCO-170ML, Operating Instructions Manual

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Phcbi MCO-170ML Operating Instructions Manual Download Page 1

Please read the operating instructions carefully before using this product, and save the operating 

instructions for future use. 

See page 101 for all model numbers.

 

 
 
 

 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

MCO-170ML

 

Operating Instructions

 

O

2

/CO

2

 Incubator 

This product is for research use only.   

Not for clinical diagnosis or treatment of humans or animals.

 

Summary of Contents for MCO-170ML

Page 1: ...using this product and save the operating instructions for future use See page 101 for all model numbers MCO 170ML Operating Instructions O2 CO2 Incubator This product is for research use only Not fo...

Page 2: ......

Page 3: ...BETTER CULTIVATION Precautions for cultures 31 Preventing contamination 32 CORRECT OPERATION 33 BASIC OPERATION ON LCD TOUCH PANEL 34 BASIC PARAMETERS Numerical input to input window 36 Setting temper...

Page 4: ...K OPTION 76 Setting User ID 76 Setting auto lock 78 Using unlock key 81 Removing auto lock 82 GAS AUTO CHANGER OPTION 83 Connecting CO2 gas cylinder 83 Automatic CO2 gas supply line changeover 84 Manu...

Page 5: ...Contact our sales representative or agent if any page of the operating instructions is lost or the page order is incorrect Contact our sales representative or agent if any point in the operating inst...

Page 6: ...lure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property Symbol shows This symbol means caution This symbol means an action is prohibited This s...

Page 7: ...and safe disposal options visit ec gc ca mercure mercury Dispose of or recycle in accordance with applicable laws Pour plus de renseignements sur les proc dures de manutention s curitaire les mesures...

Page 8: ...wer supply outlet is not grounded it will be necessary to install a ground by qualified engineers Never ground the unit through a gas pipe water main telephone line or lightning rod Such grounding may...

Page 9: ...cord Pulling the cord may result in electric shock or fire by short circuit Disconnect the power supply plug before moving the unit Take care not to damage the power supply cord A damaged cord may ca...

Page 10: ...failure or turning off of power switch The stored items may be damaged due to the change of setting Be careful not to tip over the unit during movement to prevent damage or injury Prepare a safety che...

Page 11: ...e This symbol indicates an earth This symbol means ON for a power switch This symbol means OFF for a power switch ENVIRONMENTAL CONDITIONS This equipment is designed to be safe at least under the foll...

Page 12: ...set MCO 170UVS is installed 2 When an optional electric lock MCO 170EL is installed 23 When some inner attachments are removed When 16 is removed Sample air outlet cap 17 18 19 1 20 21 22 Handles Lev...

Page 13: ...to page 18 for installing 11 Humidifying pan cover This cover prevents the UV light entering the chamber Always use it Using without it may have a bad influence on the chamber temperature distribution...

Page 14: ...Top screen will appear when the power switch is turned ON Note It takes approximately 20 seconds until Top screen is displayed During warming up Status Gas sensor initializing is displayed in the Mess...

Page 15: ...ylinder is empty Changeover is also workable by pressing this key 10 Present O2 density display field The current chamber O2 density is displayed Nothing is displayed when O2 density is set 20 11 Set...

Page 16: ...lose Door Closed is displayed Locked Door Locked is displayed 2 18 Message display field Alarms errors or messages are displayed when fault occurs Refer to page 91 94 Note When there are a number of a...

Page 17: ...the remote alarm terminals For the type and behavior of remote alarm output refer to page 91 94 The terminal of the remote alarm is installed at the rear upper right of the unit See the figure on the...

Page 18: ...boilers ovens or autoclaves Heat will adversely affect the performance of the incubator Ambient temperature at least 5 C lower than set temperature The control temperature of the incubator is at least...

Page 19: ...In addition do not install it near water or steam pipes Moisture can cause the insulation to deteriorate which may result in current leakage or electric shock No inflammable or corrosive gas Never in...

Page 20: ...he cable tie banding the power supply cord Prolonged banding may cause the corrosion of the cord coating WARNING Do not leave the plastic wrapping bags within reach of children If the bag is placed ov...

Page 21: ...ttom side of each gastight split door Fig 3 5 Adjust the leveling feet Adjust the leveling feet by turning them counterclockwise to level the incubator Fig 4 Note Incubating on a leaning tray may have...

Page 22: ...installed by qualified personnel If the incubator is not grounded it may result in electric shock Never connect the ground wire to a gas pipe water pipe lightning rod or telephone ground wire Doing so...

Page 23: ...incubator other than this product use the stacking plate MCO 170SB Refer to table 13 on page 100 When the incubator is not in use Empty the water from the humidifying pan and remove moisture from the...

Page 24: ...e gas tube tightly to prevent it from coming off Make sure that the gas tube is not folded If the CO2 gas is supplied to multiple CO2 incubators from a single gas cylinder a CO2 solid will be formed i...

Page 25: ...for the primary side and 0 25 MPa G 2 5 kgf cm2 G 36 psi G for the secondary side 2 Using a gas tube that is provided connect the connecting port A for N2 O2 gas pipe and the gas regulator of the N2...

Page 26: ...pipe is changed A to B automatically Note The gas lines connected to the incubator will degrade over time If any deterioration or abnormalities are found during inspection replace the lines immediatel...

Page 27: ...ing Be careful to keep the diluted detergent or water out of the temperature sensor the CO2 gas injection port the gas injection port the inner sample air access port the fan motor shaft bearing and t...

Page 28: ...e duct When an optional UV system set MCO 170UVS is installed 1 Turn OFF the power to the incubator 2 Open the outer and inner doors and pull out all the trays Fig 1 Note Open the inner door after clo...

Page 29: ...out the humidifying pan Fig 4 6 Pull out the fan cover Fig 5 7 Lift the duct and remove it from the pins on the rear side Fig 6 8 Pull out the fan Fig 7 Fig 5 Fig 6 Humidifying pan Duct Fan cover Fig...

Page 30: ...28 BEFORE COMMENCING OPERATION 9 Remove the silicon caps of each access port from interior Fig 8 and exterior Fig 9 Silicon cap Silicon cap Fig 9 Fig 8...

Page 31: ...sition the long hole of duct with the projection of fan cover and insert directly Fig 3 Same applies for the humidifying pan cover The fan cover may lean by strongly pushing the head of it in the back...

Page 32: ...l direction as its shorter side is placed in the back Refill the humidifying pan with water early when the volume of water is decreased Mixing any reagent in the water for humidifying may have a bad i...

Page 33: ...llage of the culture medium incomplete closing or damage to the gasket Before opening the inner door or some gastight split doors check through the glass to confirm that the UV lamp is OFF if the opti...

Page 34: ...ng them in and out Keep the chamber clean Wipe off any fingerprints If water spills from the humidifying pan or if the doors are left open for a long time condensation may form on the inside of the ga...

Page 35: ...e of the incubator 7 When an optional UV system set MCO 170UVS is installed Set the frequency of a power supply on the LCD touch panel Refer to page 66 67 WARNING Always use the removable power supply...

Page 36: ...rm Display Alarm log can be exported 57 58 Alarm Export Export Alarm log 59 61 Lock Lock screen Auto Lock Auto Lock screen 1 Setting Auto lock ON OFF User ID ON OFF 78 82 KeyLock Key Lock screen Setti...

Page 37: ...2 density or O2 density may be displayed Operation from other than Menu key CO2 gas supply line select key Operate Manual changeover between CO2 gas supply line A and B Page 86 N2 O2 gas supply line s...

Page 38: ...e and press OK key Key description Numerical key 0 9 Input numerical values Up Down key Increases or decreases the numerical value displayed in the numerical input box Clear key Deletes the numerical...

Page 39: ...2 density the O2 density and the temperature of the high limit temperature alarm for normal operation according to the following procedure The incubator automatically starts operation using these sett...

Page 40: ...ting 53 0 o C Refer to page 91 94 for detail of each alarm Note When changing the set temperature from less than 45 0 o C to 45 0 o C or higher the incubator readjusts the CO2 sensor and O2 sensor Dur...

Page 41: ...39 Setting key lock 1 Press Menu key to lead the Menu screen 2 Press Lock key to lead the Lock screen 3 Press KeyLock key to lead the Key Lock screen...

Page 42: ...ght Key Lock turns to ON Password The number Max 6 digit inputted here are registered the release password of Key Lock Confirm Password To prevent erroneous input input the same password as Password i...

Page 43: ...isplayed and input of the release password of Key Lock is required When the inputted password is incorrect Notice dialog box is displayed Press OK key and then input the correct password Removing key...

Page 44: ...Lock screen 5 On the Key Lock screen by holding Key Lock slide key and sliding to the left change to OFF Press Apply key to turn the key lock OFF The display returns to the Lock screen Note The releas...

Page 45: ...o changer MCO 21GC which changes the empty CO2 gas cylinder into the other one automatically is available as an optional accessory refer to page 83 86 Refer to page 23 24 for connecting N2 gas cylinde...

Page 46: ...pty it changes into N2 gas cylinder A Table 2 N2 O2 gas supply line automatic changeover e g When N2 gas cylinder A is empty it changes over N2 gas cylinder B Situation N2 gas N2 O2 gas supply line in...

Page 47: ...line A is changed to B by N2 gas O2 gas automatic changer function the N2 O2 gas supply line B is changed to A manually without pressing the Buzzer key Table 3 Situation N2 gas N2 O2 gas supply line i...

Page 48: ...ead the Alarm Setting 1 screen it is possible to set automatic set temperature alarm automatic set CO2 density alarm automatic set O2 density alarm and alarm delay of each alarm go to procedure 4 on p...

Page 49: ...ettable range 0 5 5 0 factory setting 1 0 Automatic set CO2 density alarm delay CO2 Alarm Delay The function is that when the incubator is in the alarm state of automatic set CO2 density the alarm buz...

Page 50: ...minutes Note At Err01 CO2 gas cylinder empty Err02 N2 O2 gas cylinder empty Err11 12 CO2 sensor error Err18 UV lamp failure and Door alarm the alarm is not re activated because the alarm itself is de...

Page 51: ...log data chamber temperature CO2 density O2 density and open close state of outer door Use the following procedure to set the log interval interval of acquiring the operation log 1 Press Menu key to l...

Page 52: ...etween log interval and the estimated amount of data that can be saved Log interval 2 minutes Approx 46 days Log interval 6 minutes Approx 135 days Log interval 30 minutes Approx 664 days When saving...

Page 53: ...een by pressing Show key after pressing the item you want to display graphically the graph of each operation log is displayed Actual Temp Chamber temperature log graph Go to procedure 6 Actual CO2 Lev...

Page 54: ...the Chart screen Press Top key to return to the Top screen 9 Door Opening log graph is displayed Press Back key to return to the Chart screen Press Top key to return to the Top screen Note The error...

Page 55: ...he USB memory into the USB port Note It is not possible to use a USB memory with security functions that requires entering password 11 Press Export key 12 When the export is complete Information dialo...

Page 56: ...to the USB memory inserted into the USB port 1 Insert the USB memory into the USB port Note It is not possible to use a USB memory with security functions that requires entering password 2 Press Menu...

Page 57: ...log data you want to export To export all types of operation log data press All Ch key To export only operation log data you want to export select operation log data you want to export and then press...

Page 58: ...er is created in the USB memory and the exported file is saved in it in CSV format The exported file name is in date 8 digits type of data format e g When exporting all types of data using All from Oc...

Page 59: ...Alarm log saved in the incubator can be displayed graphically on the LCD touch panel Note When saving alarm logs more than 257 the oldest alarm log is deleted and then overwritten 1 Press Menu key to...

Page 60: ...range 1 day 45 days Note The error of about 1 minute may be observed during 1 month Refer to page 62 for the procedure of setting time Press Back key to return to the Log screen Press Top key to retur...

Page 61: ...the USB port by CSV format 1 Insert a USB memory in the USB port Note It is not possible to use a USB memory with security functions that requires entering password 2 Press Menu key to lead the Menu s...

Page 62: ...button and input days Settable range 1 day 45 days Note The error of about 1 minute may be observed during 1 month Refer to page 62 for the procedure of setting time 6 Press Export key Note When USB m...

Page 63: ...deleted 8 Remove a USB memory from the USB port Note A log folder is created in a USB memory and an exported data file is saved in the log folder by CSV format Exported file name The first date durin...

Page 64: ...key to lead the Date Time screen 4 On the Date Time screen input the present date and time Press Apply key to save the input value The display returns to the Tools 2 screen Note 24 hour clock It is re...

Page 65: ...Tools 2 key to lead the Tools 2 screen 3 Press Brightness Sleep key to lead the Brightness Sleep screen 4 On the Brightness Sleep screen each setting of brightness and sleep is available Press Apply...

Page 66: ...right the Sleep function is turned to ON Input the set value of time to change the Sleep state Settable range 1 minute 5 minutes factory setting 2 minutes Note It is not possible to operate any key in...

Page 67: ...he chamber and chamber temperature distribution because the UV lamp generates heat for a long time It may also shorten the service life of the UV lamp The preset period can be changed when necessary a...

Page 68: ...information on replacing the UV lamp If the UV lamp burns out Err18 UV Lamp Abnormal is displayed in the message display field it is not possible to perform H2O2 decontamination Replace the UV lamp a...

Page 69: ...lamp has turned on is displayed as the percentage to 5 000 hours which are recommendation time to replace It is impossible to set UV Timer Ext The more total time which UV lamp has turned on increases...

Page 70: ...cover the duct the fan the humidifying pan the humidifying pan cover the gas injection nozzle and the gas injection nozzle tube Clean all the attachments in an autoclave or with alcohol for disinfect...

Page 71: ...n display when UV lamp is lighting Note The UV 24 hour mode may cause the automatic set temperature alarm because of a rising chamber temperature After procedure 8 by opening the outer door when UV la...

Page 72: ...inner door and the outer door are securely closed During H2O2 decontamination plug the access hole with the silicon caps that are provided Failure to do so may be harmful to health due to leakage of H...

Page 73: ...H2O2 generator in the 2 holes on the lower left side of the duct Fig 1 Note Make sure that the H2O2 generator is securely attached Uncertain attachment may result in insufficient decontamination 7 Re...

Page 74: ...gastight split doors the inner door and the outer door 11 Press H2O2 key for 3 seconds to open Setting Position window Note When the model does not have H2O2 decontamination function H2O2 key is not d...

Page 75: ...zzer sounds when H2O2 decontamination is completed About 100 minutes later CAUTION The electric lock will remain locked if power supply is cut off during H2O2 decontamination After the power supply is...

Page 76: ...and inner doors Disconnect connector of the chamber cable and H2O2 generator and remove H2O2 generator and cable from the chamber Note When doing the above work put on protection glasses and rubber g...

Page 77: ...er cover securely to prevent impurities from becoming mixed in the reagent Check the container to make sure that there is no damage corrosion or cracking Store the container with the inlet facing upwa...

Page 78: ...ing the outer door are as follows Quick mode Press the Unlock key User ID mode Input the User ID and release password of Auto Lock after pressing the Unlock key Setting User ID Before turning the User...

Page 79: ...ord as Password input box When inputting a different password Notice dialog box is displayed Press OK key and input the correct password Note A release password of Auto Lock is for unlocking the outer...

Page 80: ...registered User ID into User ID input box and input its registered password into Password input box Press Delete key to delete the registered User ID and its password Note When deleting all registered...

Page 81: ...of unlocking the outer door between the quick mode or the User ID mode By holding the User ID slide key and sliding it right the User ID mode is turned to ON Factory setting OFF quick mode Note When n...

Page 82: ...ID and its release password of Auto Lock Note The User ID which is inputted at this time is saved as the open close state of outer door log data refer to page 54 56 When the inputted User ID or its p...

Page 83: ...ed use the unlock key that is provided To re lock the outer door turn the unlock key to the lock direction while the outer door is open Close the outer door after the out door is locked manually Note...

Page 84: ...d the Lock screen 3 Press Auto Lock key to lead the Auto Lock screen 4 On the Auto Lock screen by holding the Auto lock slide key and sliding it left the Auto lock is turned to OFF Press Apply key to...

Page 85: ...he CO2 gas cylinder A 3 Using a gas tube that is provided connect the connecting port B for CO2 gas pipe and the gas regulator of the CO2 gas cylinder B Note If the CO2 gas is supplied to multiple CO2...

Page 86: ...o changer MCO 21GC is installed CO2 gas supply line indicator A B and CO2 gas supply line select key are displayed in the Top screen CO2 gas supply line indicator A or B being used is lighted CO2 gas...

Page 87: ...as cylinder A Table 4 CO2 gas supply line automatic changeover e g When CO2 gas cylinder A is empty it changes over CO2 gas cylinder B Situation CO2 gas CO2 gas supply line indicator Message display f...

Page 88: ...gas supply line A is changed to B by CO2 gas automatic changer function the CO2 gas supply line B is changed to A manually without pressing the Buzzer key Table 5 Situation CO2 gas CO2 gas supply line...

Page 89: ...incubator Since a Standard gas cylinder is used as a standard of exact density during CO2 O2 density calibration prepare a standard gas cylinder that is same as the set CO2 O2 density Note There is no...

Page 90: ...ing 5 0 ON O2 standard gas density O2 STD Gas Density O2 density of the connected standard gas cylinder By holding O2 STD Gas Density slide key and sliding it to the left O2 density calibration turns...

Page 91: ...ation starts Calibration go to Step5 Procedure 10 automatically 10 After completion of gas density calibration display leads the STD Gas Calibration Step5 screen O2 CO2 incubator returns to the normal...

Page 92: ...st once a month 1 Remove all the inner attachments by the procedures shown on page 26 28 2 Clean the chamber and all the inner attachments by the procedures shown on page 25 3 Install all the inner at...

Page 93: ...ration for approx 90 s Return to the Top screen Door alarm The outer door is open Door Open is displayed alternately in normal characters and reverse video in the outer door opening closing display In...

Page 94: ...is short circuited Err15 Door Heater Abnormal is displayed in the message display field Sensor box heater error 1 a High limit temperature alarm is activated b The sensor box heater burnout occurs or...

Page 95: ...hen the Ring Back set time passes ON Non interlock with Buzzer key ON ON ON Under continuation ON Continue ON Under continuation OFF OFF Interlock with Buzzer key ON OFF Note Close the outer door when...

Page 96: ...lay field Continuous tone when the outer door is open ON when the outer door is open H2O2 mist generation is cancelled Resolve time is extended Failure during H2O2 gas resolve by UV The UV lamp failed...

Page 97: ...there a gas leak Has the CO2 O2 gas density setting been changed Is the gas cylinder empty Check the primary pressure of the gas cylinder once a week When the primary pressure is 3 8 MPa G 38 kgf cm2...

Page 98: ...power failure The gas density is slow to recover A HEPA filter is used for the incubator gas piping If gas density is slow to recover when the gas pressure is normal it is possible that the HEPA filt...

Page 99: ...tor securely installed Set the 2 pins of the H2O2 generator in the 2 holes at the left bottom of the duct Fig 3 Is it the end of life of the H2O2 generator If the total operating time exceeds 5 000 ho...

Page 100: ...by qualified personnel only If the O2 CO2 incubator is left where outsiders enter it may result unexpected accident for example children to become locked inside Before disposing the O2 CO2 incubator w...

Page 101: ...ture display Digital display CO2 controller PID control system CO2 density display Digital display O2 controller PID control system O2 density display Digital display Air circulation Fan assisted Air...

Page 102: ...ta acquisition system MTR 5000 user Note Refer to the updated catalog when ordering an optional component Designs and specifications are subject to change without notice Table 12 Optional accessories...

Page 103: ...control range 1 to 18 and 22 to 80 O2 variation 0 2 ambient temperature 23 o C setting 37 o C CO2 5 O2 5 no load Chamber humidity 95 R H 5 R H Applicable environment condition Temperature 5 o C to 35...

Page 104: ...the unit Unit interior No contamination Decontaminated Contaminated Others 3 Instructions for safe repair maintenance disposal of the unit a The unit is safe to work on b There is some danger see belo...

Page 105: ......

Page 106: ...Printed in Indonesia LDCL042600 0 N0418 0 1 1 1 Sakada Oizumi machi Ora gun Gunma 370 0596 Japan PHC Corporation 2018...

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