PerkinElmer Lambda XLS, User Manual

Page: 57 / 90

Instrument Methods . 57
4. Enter the number of
Standards
to be used in the standards curve using the keypad
numbers or left and right arrows, and then press the down arrow.
The options are 1–9.
5. Enter the
Units
and then press the down arrow.
You can enter text up to 8 characters long using the keypad, or press Options to
display the Units screen and access a list of pre-defined units.
In the Units screen, use the left and right arrows to select from the available options.
The options are
µg/ml, µg/µl
,
pmol/µl
,
mg/dl
,
mmol/l
,
µmol/l
,
g/l
,
mg/l
,
µg/l
,
U/l
,
%
,
ppm
,
ppb
,
conc
or none.
The Units screen also enables you to select the number of displayed decimal points
(
DP
). The options are
Auto
,
0
,
1
and
2
.
NOTE: The result will always be fixed to 5 significant figures, regardless of how many
decimal points are selected (so 98768.2 is displayed as 98768, even with 1 decimal
point selected).
Press
OK
to save your options and return to the Method Settings screen.
You can still edit the units.
6. Select the type of
Curve Fit
using the left and right arrows, and then press the down
arrow.
The options are
Zero Regression
(this forces the straight line through the origin),
Regression
(straight-line regression),
Interpolation
(linear interpolation) or
Cubic Spline
.
7. Select the
Calibration
mode using the left and right arrows.
The options are
Standards
(to measure prepared standards) or
Manual
(entering
absorbance values by keypad) or
New Standards
(to replace any standards that have
already been collected).
8. If you selected
Standards
, press the down arrow and enter the number of
Replicates
.
This determines the number of standards to be measured and averaged at each
standard concentration point.
The options are
Off
(1),
2
or
3
.
NOTE: If you selected
Manual
in Step 7, the option
Replicates
is not available.