PerkinElmer Lambda 25 User Manual Download Page 101

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Lambda 25, 35, 45 User’s Guide

100

Figure 24 Lamp baffle

3. Loosen the thumbscrew holding the attenuator in place.

Figure 25 Deuterium lamp assembly

Thumbscrew

Lamp energy
attenuator

Deuterium lamp
assembly

Thumbscrew

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Summary of Contents for Lambda 25

Page 1: ...l service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE INSPECTION Remotely inspect equipment before purchasing with our interactive website at www instraview com LOOKING FOR MORE INFORMATION Visit us on the web at www artisantg com for more information on ...

Page 2: ...Lambda 25 35 45 User s Guide Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 3: ...for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of PerkinElmer Inc Copyright 2000 P...

Page 4: ...45 17 Labels 18 Introduction 21 About this Manual 23 Conventions Used in this Manual 24 Definitions 24 Notes cautions and warnings 24 Other Warnings used in this Manual 27 System Description 35 Introduction 37 Features 37 Optical System of the Lambda 25 35 45 38 Lambda 25 Specification 41 General 41 Optics 42 Abscissa 42 Ordinate 43 Data Output 44 Lambda 35 Specification 45 General 45 Optics 46 Ab...

Page 5: ...V WinLab 60 Startup and Shutdown 61 Startup 61 Shutdown 62 Installing the Single Cell Holder 63 Description 63 Accessories 71 Accessory Installation 73 Accessory Connector Panel 74 Accessory Connections Overview 76 Sample Compartment Cover 76 Sample Compartment Front Cover 77 Sample Compartment Window 77 Linear Transporter 78 Installation Overview 78 Temperature Sensor 79 Installation Overview 79 ...

Page 6: ...amp Replacement 93 Deuterium Lamp Replacement 96 Lamp Energy Attenuator 99 Lamp Alignment Procedure 101 Changing Fuses 102 Replacement Parts 104 Analytical Notes 105 Unusual Samples 107 Volatile Samples 107 Samples not Governed by the Beer Lambert Law 107 Chemically Reactive Samples 108 Photoactive Samples 108 Other Sample Properties 108 Solvent Properties 109 Index 111 Index 113 Artisan Technolog...

Page 7: ...Lambda 25 35 45 User s Guide 6 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 8: ...Warnings and Safety Information 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 9: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 10: ... supercede the normal safety code of behavior prevailing in the user s country IEC 61010 Compliance This instrument has been designed and tested in accordance with IEC 1010 1 Safety requirements for electrical equipment for measurement control and laboratory use and Amendment 1 to this standard CSA Compliance This instrument meets the Canadian Standards Association CSA Standard CAN CSA C22 2 1010 ...

Page 11: ...trical Safety To ensure satisfactory and safe operation of the instrument it is essential that the green yellow lead of the line power cord is connected to true electrical earth ground If any part of the instrument is not installed by a PerkinElmer service representative make sure that the line power plug is wired correctly Cord Lead Colors Terminal International USA Live Brown Black Neutral Blue ...

Page 12: ...ay still be charged even if the instrument has been disconnected from all voltage sources Connect the instrument to a correctly installed line power outlet that has a protective conductor earth ground Do not attempt to make internal adjustments or replacements except as directed in this handbook Do not operate the instrument with any covers or parts removed Servicing should be carried out only by ...

Page 13: ...it against any unauthorized or unintentional operation The instrument is likely to be electrically unsafe when it Shows visible damage Fails to perform the intended measurement Has been subjected to prolonged storage under unfavorable conditions Has been subjected to severe transport stresses WARNING If the equipment is used in a manner not specified herein the protection provided by the equipment...

Page 14: ... 4 5 IEC 61000 4 6 IEC 61000 4 11 This product complies with EN 55011 Group 1 Class A and IEC 61000 3 2 FCC rules and regulations This product is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Artisan Technology Group Quality ...

Page 15: ...hout condensation Altitude in the range 0 m to 2000 m The instrument has been designed to be safe under the following environmental conditions Indoor use Ambient temperatures of 5 ºC to 40 ºC A maximum ambient relative humidity of 80 for temperatures up to 31 o C decreasing linearly to 50 relative humidity at 40 o C Altitude up to 2000 m above mean sea level Mains supply fluctuations not exceeding...

Page 16: ...rkplace and instrument operators are not exposed to hazardous levels of toxic substances chemical or biological as defined in the applicable Material Safety Data Sheets MSDS or OSHA ACGIH or COSHH documents Venting for fumes and disposal of waste must be in accordance with all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration U S A A...

Page 17: ...ained under reduced pressure When you dispose of lamps that are defective or otherwise unusable handle them correctly to minimize the implosion risk UV Radiation You should be aware of the health hazards presented by ultraviolet radiation When the deuterium UV lamp is illuminated do not open the spectrophotometer covers unless specifically instructed to do so in the manual Always wear UV absorbing...

Page 18: ...d by safety advisors before attempting to lift the instrument As the Lambda 25 35 45 weighs approximately 26 Kg we recommend that the instrument is lifted by 2 adults and that it is lifted by the base of the instrument Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 19: ... 1 Labels on the spectrometer housing The following electrical warnings are shown on the rear of the instrument Figure 2 Warning labels on the rear of the instrument Serial number Electrical warnings Serial number inside the sample compartment Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 20: ...of lamp compartment The following warnings are shown on the inside of the lamp compartment Figure 4 Warnings on the inside of the lamp compartment Warning labels shown on the inside of the lamp compartment Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 21: ...Lambda 25 35 45 User s Guide 20 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 22: ...Introduction 2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 23: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 24: ...al contains the following sections Warnings and Safety Information Introduction System Description Installation Accessories Maintenance Analytical Notes Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 25: ...Definitions Responsible body individual or group responsible for the use and maintenance of equipment and for ensuring that operators are adequately trained Operator person operating equipment for its intended purpose Notes cautions and warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings NOTE A note indicates additional significant i...

Page 26: ...ste tipo de circunstancias F Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoquer de graves détériorations de l instrument ou d autre matériel Les détails sur ces circonstances figurent dans un encadré semblable à celui ci I Caution Attenzione Con il termine CAUTION ATTENZIONE vengono segnalate situazioni che potrebbero arrecare gravi d...

Page 27: ...rmación sobre este tipo de circunstancias F Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages corporels à l utilisateur ou à d autres personnes Les détails sur ces circonstances sont données dans un encadré semblable à celui ci I Warning Pericolo Con il termine WARNING PERICOLO vengono segnalate situazioni che potrebbero provocare ...

Page 28: ...Introduction 27 Other Warnings used in this Manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 29: ...Lambda 25 35 45 User s Guide 28 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 30: ...Introduction 29 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 31: ...Lambda 25 35 45 User s Guide 30 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 32: ...Introduction 31 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 33: ...Lambda 25 35 45 User s Guide 32 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 34: ...Introduction 33 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 35: ...Lambda 25 35 45 User s Guide 34 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 36: ...System Description 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 37: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 38: ...re a double beam all reflecting system Lambda 25 35 45 spectrometers are usable in a wide range of applications as indicated by their performance specification Features Figure 5 Lambda 25 35 45 Spectrometer Features Lamp compartment Power switch Connector panel Sample compartment Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 39: ...adiation from the deuterium lamp NOTE In the Lambda 45 M2 is substituted by a pre monochromator grating increasing the straylight performance of the instrument For operation in the ultraviolet UV range mirror M1 is raised to permit radiation from the deuterium lamp to strike source mirror M2 Source change is automatic during monochromator slewing Radiation from the source lamp is reflected from so...

Page 40: ...egment through the exit slit Slit 2 to mirror M3 Figure 6 Optical Path for Lambda 25 35 45 replaced with a pre monochromator in the Lambda 45 selectable on the Lambda 35 45 Halogen lamp Deuterium lamp Filter wheel M2 M1 Slit 1 Slit 2 M5 Reference Sample M3 Detector Lens Lens Detector M4 Grating Monochromator Beam Splitter M Mirror M1 M4 and M5 Plane Mirror M2 Toroidal Mirror M3 Spherical Mirror Ar...

Page 41: ...onto a beam splitter which allows 50 of the radiation to pass onto plane mirror M4 and reflects 50 of the radiation onto plane mirror M5 Mirror M4 focuses the radiation beam in the sample cell The beam then passes through a convex lens onto the photodiode detector Mirror M5 focuses the radiation beam in the reference cell The beam then passes through a convex lens onto the photodiode detector Arti...

Page 42: ...ient operating temperature 15 ºC to 35 ºC Humidity range 20 to 80 relative humidity without condensation Technical Standard In compliance with the requirements for technical instruments stipulated by IEC 61010 1 1990 and amendments A1 1992 and A2 1995 Radio interference suppression In compliance with the legal requirements of the EMC directive 89 336 EEC EN 61326 Artisan Technology Group Quality I...

Page 43: ...er Radiation sources Prealigned deuterium and halogen lamps Detector Photodiodes One for the sample beam and one for the reference beam Abscissa Wavelength range 190 nm to 1100 nm 0 nm for alignment purposes Wavelength accuracy 0 1 nm Wavelength reproducibility 0 1 nm Spectral bandwidth 1 nm fixed slit Lamp change Automatically at 326 nm software selectable over the whole wavelength range Scan spe...

Page 44: ...nm 340 nm and 370 nm Absorbance 2 measured at 200 nm with potassium chloride solution against distilled water Baseline linearity 1 nm slit Absorbance 0 001 corrected 200 nm to 1100 nm scan speed 240 nm min smooth 2 Baseline noise 1 nm slit Absorbance 0 00008 RMS Absorbance 0 0003 peak to peak 3 min at absorbance 0 wavelength 500 nm and response 2 s Baseline stability drift Absorbance 0 0003 per ho...

Page 45: ...Lambda 25 35 45 User s Guide 44 Data Output Digital port One RS 232 C interface serial for connecting a PC Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 46: ...ient operating temperature 15 ºC to 35 ºC Humidity range 20 to 80 relative humidity without condensation Technical Standard In compliance with the requirements for technical instruments stipulated by IEC 61010 1 1990 and amendments A1 1992 and A2 1995 Radio interference suppression In compliance with the legal requirements of the EMC directive 89 336 EEC EN 61326 Artisan Technology Group Quality I...

Page 47: ...c concave grating with 1053 lines mm in the center Radiation sources Prealigned deuterium and halogen lamps Detector Photodiodes One for the sample beam and one for the reference beam Abscissa Wavelength range 190 nm to 1100 nm 0 nm for alignment purposes Wavelength accuracy 0 1 nm Wavelength reproducibility 0 1 nm Spectral bandwidth 0 5 nm 1 nm 2 nm 4 nm selectable fixed slit widths Lamp change A...

Page 48: ...nm 340 nm and 370 nm Absorbance 2 measured at 200 nm with potassium chloride solution against distilled water Baseline linearity 2 nm slit Absorbance 0 0005 corrected 200 nm to 1050 nm scan speed 240 nm min smooth 2 Baseline noise 2 nm slit Absorbance 0 00006 RMS Absorbance 0 0002 peak to peak 3 min at Absorbance 0 wavelength 500 nm and response 2 s Baseline stability drift Absorbance 0 0003 per h...

Page 49: ...Lambda 25 35 45 User s Guide 48 Data Output Digital port One RS 232 C interface serial for connecting a PC Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 50: ...ient operating temperature 15 ºC to 35 ºC Humidity range 20 to 80 relative humidity without condensation Technical Standard In compliance with the requirements for technical instruments stipulated by IEC 61010 1 1990 and amendments A1 1992 and A2 1995 Radio interference suppression In compliance with the legal requirements of the EMC directive 89 336 EEC EN 61326 Artisan Technology Group Quality I...

Page 51: ...c concave grating with 1053 lines mm in the center Radiation sources Prealigned deuterium and halogen lamps Detector Photodiodes One for the sample beam and one for the reference beam Abscissa Wavelength range 190 nm to 1100 nm 0 nm for alignment purposes Wavelength accuracy 0 1 nm Wavelength reproducibility 0 1 nm Spectral bandwidth 0 5 nm 1 nm 2 nm 4 nm selectable fixed slit widths Lamp change A...

Page 52: ...nm 340 nm and 370 nm Absorbance 2 measured at 200 nm with potassium chloride solution against distilled water Baseline linearity 2 nm slit Absorbance 0 0005 corrected 200 nm to 1100 nm scan speed 240 nm min smooth 2 Baseline noise 2 nm slit Absorbance 0 00006 RMS Absorbance 0 0002 peak to peak 3 min at Absorbance 0 wavelength 500 nm and response 2 s Baseline stability drift Absorbance 0 0003 per h...

Page 53: ...Lambda 25 35 45 User s Guide 52 Data Output Digital port One RS 232 C interface serial for connecting a PC Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 54: ...Installation 4 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 55: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 56: ...from dust and corrosive fumes Keep out of direct sunlight Illumination with diffuse lighting is ideal A suitable source of electrical power should be located in the vicinity of the instrument Electrical power must be available at a proper earth grounded 3 wire electrical outlet The standard sample compartment baseplates have drain holes in them to run off spilled liquids to the benchtop underneath...

Page 57: ...Lambda 25 35 45 User s Guide 56 Figure 7 Space Requirements Front view Right side view 56 cm 65 cm 26 cm Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 58: ...nents listed below are provided with the spectrometer Quantity Item Part Number 2 Single cell holders Lambda 25 35 45 B0505071 1 Screwdriver 4 mm B0126972 1 Hexagonal socket head wrench 3 mm B0140883 10 Spare fuses B0155573 1 Line power cord with suitable connector for electrical outlets varies 1 Lambda 25 35 45 manuals kit 09934419 1 9 pin RS232 cable 09410022 1 UV WinLab software kit L6100013 Ar...

Page 59: ...re not damaged Open and close the sample compartment cover checking that it moves freely without binding The compartment must be free of dust or other foreign matter In the event of damage or missing parts file an immediate claim with the authorized carrier and inform your PerkinElmer office or representative NOTE PerkinElmer offers an installation service for your spectrometer Contact your local ...

Page 60: ...r of the spectrometer see Changing Fuses on page 102 Voltage Standard 100 V 120 V 6 3 A slow blow 210 V 240 V 3 15 A slow blow NOTE The module has two fuses 2 Make sure that the plug fitted to the line power cord provided with the spectrometer is suitable for your local electrical outlets 3 After all connections have been made between the various components of the system make certain that all powe...

Page 61: ...the PC to the spectrometer using the cable provided 09410022 as shown in Figure 8 Figure 8 Connecting the PC to the spectrometer Installing UV WinLab To install UV WinLab on the PC follow the instructions in the UV WinLab Software User s Guide PC RS232 COM1 2 10101 1 09410022 supplied Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 62: ... that is No objects for example cables project into the beam paths No samples are in the sample compartment Accessories are properly installed NOTE If the sample compartment is obstructed during the startup procedure the spectrometer will not initialize correctly 3 Close the sample compartment cover 4 Switch on the power switch 5 Wait for approximately 3 minutes until all initialization is complet...

Page 63: ...nt cover 4 Remove samples and cells from the sample compartment 5 If accessories for example flowcell are installed in the sample compartment clean them thoroughly 6 Close the sample compartment cover 7 Switch off the spectrometer Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 64: ...ption Figure 9 Single Cell Holder B0505071 Locking screw for horizontal alignment Vertical adjustment screw Milled post Lifter Locking screw for horizontal alignment Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 65: ...ell holder can be installed in two different positions in the sample compartment Always install the holder such that the arrow on the cell holder lines up with the center point on the baseplate Install the single cell holder in the sample compartment as follows 1 Orientate the holder so that the lifter is toward the rear of the sample compartment 2 Lower the holder so that the two alignment holes ...

Page 66: ...lder Coarse alignment of the single cell holder is carried out as follows 1 Start the system as described in Startup on page 61 2 Open the sample compartment cover 3 Fill matching cells with a low absorbing solvent deionized water or ethanol 4 Insert one cell into the sample cell holder and one into the reference cell holder Make certain that the cell is pushed down fully NOTE The alignment proced...

Page 67: ...become apparent if the radiation beam impinges on the cell wall 11 If the radiation beam is not centered exactly loosen the two locking screws and the two milled posts on the relevant cell holder and shift the cell holder plate to center the radiation beam horizontally Then retighten the two milled posts and the two locking screws 12 Now visually check the vertical alignment of the radiation beam ...

Page 68: ...og is displayed 16 Set the Wavelength to any value above 200 nm and click Setup 17 Remove the card blocking the sample beam window and close the sample compartment cover This completes the coarse alignment of the cell holder If necessary proceed with the fine alignment as described below Fine Alignment If fine alignment is necessary proceed as follows 1 From the Application menu in UV WinLab selec...

Page 69: ...adjustment screw again to obtain the highest possible reading close sample compartment cover while measuring transmittance 9 When you are satisfied with the alignment tighten the milled posts and the locking screws on the cell holder 10 Insert the matching cell with the same low absorbing solvent into the reference cell holder The first cell remains in the sample cell holder 11 Repeat steps 5 to 9...

Page 70: ...thlength Minimum Volume Required Part Number 0 5 µL 0 01 cm 2 µL B0631082 2 5 µL 0 5 cm 5 µL B0631080 5 µL 0 1 cm 10 µL B0631083 5 µL 1 0 cm 10 µL B0631081 Cell window completely filled with liquid 30 µL 1 0 cm 50 µL B0631079 NOTE You should align microcells very carefully in the radiation beam by following the procedures in Aligning the Single Cell Holder on page 65 When aligning microcells fill ...

Page 71: ...Lambda 25 35 45 User s Guide 70 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 72: ...Accessories 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 73: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 74: ...ctive accessory To install certain accessories you need to remove the sample compartment cover The required procedures are described below In the directions provided with some sample handling accessories reference is made to earlier models in the Lambda Series of spectrometers These directions are generally applicable to the current series of instruments since the sample compartment is standardize...

Page 75: ...mmon connector panel is used for the Lambda Series of instruments Figure 12 Accessory Connector Panel PELTIER J30 TEMPERATURE J11 J10 J14 J1 AUTOSAMPLER ACCY SPHERE J88 P C Accessory panel Standard configuration Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 76: ...ger 13 Cell autochanger Active J10 J11 Temp Temperature sensor J10 2 pin socket J11 4 pin socket J30 Peltier Peltier cell changer and Peltier cell holder Active J88 Sphere Integrating Sphere Active J1 Pump for dissolution Active Autosampler AS 93plus Autosampler RS 232 C Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 77: ...1 Open the cover only about 90 degrees to the sample compartment 2 Carefully slide the cover straight up off both hinges to remove To install the sample compartment cover or other accessory cover perform this procedure in reverse J J J PC Multi sipper Linear Transporter AS 93plus Autosampler Peltier Cell Changer Temperature Sensor Peltier Cell Holder Integrating sphere Artisan Technology Group Qua...

Page 78: ... cover of the sample compartment 3 Slide the front cover forwards and remove from the spectrometer To install the sample compartment front cover perform this procedure in reverse Sample Compartment Window To remove or install certain accessories you need to remove the sample compartment windows Each window has a magnetic frame and should be carefully removed by hand Artisan Technology Group Qualit...

Page 79: ...ne power supply before you alter or make any new electrical connections Installation Overview Connector on Spectrometer Accy Installation See linear transporter manual Cables Lead through bottom of spectrometer housing Tubes Lead through the tube ports Sample Compartment Cover Unchanged Alignment See linear transporter manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURC...

Page 80: ...n Overview NOTE When connecting the temperature sensor align the red mark on the plug with the red mark on the socket Pull the collar on the plug back to connect disconnect the plug Release the collar to secure the plug see Figure 12 page 74 Connector on Spectrometer Temp J10 Installation See temperature sensor description Cables Lead through the tube ports Sample Compartment Cover Unchanged Artis...

Page 81: ...line power supply before you alter or make any new electrical connections Installation Overview Connector on Spectrometer Accessory Port Installation See Multisipper manual Cables See Multisipper manual Tubes See Multisipper manual Sample Compartment Cover Unchanged Figure 14 Multisipper electrical connection B0167808 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www ar...

Page 82: ... supply before you alter or make any new electrical connections Installation Overview Connector on Spectrometer Autosampler Installation See autosampler manual Cables See autosampler manual Tubes See autosampler manual Figure 15 Autosampler electrical connection AS 93plus Autosampler Spectrometer External P S U Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg...

Page 83: ...be detected automatically and the appropriate window in UV WinLab Method will be displayed Accessories Samples can be applied either manually or with the help of a number of accessories The following accessories are currently available Changers 9 Cell Changer Peltier thermostatted 13 Cell Changer Linear Transporter With 8 cell holder With 5 cell long pathlength holder With 6 cell holder Gel plate ...

Page 84: ...be activated on the accessory page of the UV WinLab software The connector panel for the accessory in question must be installed in the spectrometer Some accessories such as Peltier cell holder and 9 cell Peltier changer require an upgrade board fitted to the power supply unit Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 85: ...Lambda 25 35 45 User s Guide 84 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 86: ...Maintenance 6 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 87: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 88: ...t except as described in the accompanying User Documentation Only a PerkinElmer service representative or similarly trained and authorized person should be permitted to service the instrument Daily Care WARNING Switch off the instrument before cleaning any spilled materials The instrument is constructed with high quality components and requires little maintenance other than to keep it clean and fr...

Page 89: ... the end of the working day Generally such systems should be filled with deionized water when left overnight CAUTION Risk of damage to Optics or Electronics Take care not to spill liquids onto the spectrometer Expensive damage can result to the optics or electronics if liquids are spilled and run inside the instrument or onto the keyboard Cleaning the Sample Compartment You must clean the sample c...

Page 90: ...ectral range of the spectrometer The window seals the sample compartment and thus protects the instrument s optics from dust and fuming or aggressive samples Generally the window should be installed at all times The window is an optical component and requires the same care and handling as cells You can remove the window to clean it The frame is magnetic and can be removed by hand Windows are most ...

Page 91: ... from scratches and never permit them to rub against one another or against other hard surfaces Avoid abrasive corrosive or stain producing cleaning agents and make certain that the exposed surfaces of cells are optically clean Always wipe the optical surfaces of cells dry and free of fingermarks using a soft cloth or cleaning tissue just before placing them in the cell holder When measuring cold ...

Page 92: ...r any slight increase in pressure in the cell during routine operation If for analytical reasons it is necessary to fill the cell completely insert the stopper only lightly so that the liquid in the cell has a chance to expand Do not insert a stopper forcefully into a completely filled cell since this is likely to cause the stopper to pop out of the cell When working at higher temperatures use a d...

Page 93: ...igure 17 Inside the Lamp Compartment Baffle removed Lamp compartment Halogen lamp assembly Lamp energy attenuator White ceramic connector Deuterium lamp connector Deuterium lamp assembly Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 94: ...NING Electrical Hazard Switch off the spectrometer and remove the plug from the electrical supply before starting with the replacement Risk of Burns If the old lamp was lighted allow it to cool before proceeding with the replacement UV Radiation The lamps emit intense radiation which can damage your eyes Do not open the lamp compartment when the lamps are on Do not gaze into a lighted lamp Artisan...

Page 95: ...hing the cover to the left 3 Remove the lamp baffle by slackening the thumbscrew for the deuterium lamp and lifting the lamp baffle vertically upward Figure 19 Lamp baffle 4 Carefully pull the white ceramic connector from the rear of the halogen lamp Thumbscrew Thumbscrew Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 96: ...acket in the lamp compartment and then secure with the thumbscrew 8 Carefully push the ceramic connector firmly onto the pins on the base of the lamp 9 Wipe the bulb with a soft cloth moistened with alcohol to remove dirt since this would otherwise be burned in when the lamp is hot 10 Replace the lamp baffle using the reverse of the procedure described in step 3 11 Replace the lamp compartment cov...

Page 97: ...ible to read off the number of hours that the lamp has been in operation One scale division corresponds to approximately 100 hours WARNING Electrical Hazard Switch off the spectrometer and remove the plug from the electrical supply before starting with the replacement Risk of Burns If the old lamp was lighted allow it to cool before proceeding with the replacement UV Radiation The lamps emit inten...

Page 98: ...baffle by slackening the thumbscrews and lifting the lamp baffle vertically upward Figure 22 Lamp baffle 4 Unplug the deuterium lamp connector from the terminal board by squeezing in the two lugs at each side of the connector and carefully pulling the connector vertically upward Thumbscrew Thumbscrew Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 99: ...onnector into the socket NOTE The socket in the lamp compartment is asymmetric the deuterium lamp connector can be inserted in one direction only Make certain that the connector is the right way round before inserting it Never attempt to insert the connector by force 9 Wipe the lamp window with a soft cloth moistened with alcohol to remove dirt since this would otherwise be burned in when the lamp...

Page 100: ...emove the plug from the electrical supply before starting with the replacement Risk of Burns If the old lamp was lighted allow it to cool before proceeding with the replacement UV Radiation The lamps emit intense radiation which can damage your eyes Do not open the lamp compartment when the lamps are on Do not gaze into a lighted lamp Operate the attenuator as follows 1 Open the lamp compartment 2...

Page 101: ...le 3 Loosen the thumbscrew holding the attenuator in place Figure 25 Deuterium lamp assembly Thumbscrew Lamp energy attenuator Deuterium lamp assembly Thumbscrew Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 102: ...beam 6 Replace the lamp baffle using the reverse of the procedure described in step 2 7 Close the lamp compartment Lamp Alignment Procedure Due to the prealigned mounts the alignment of lamps after installation is generally so good that further alignment is not required Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 103: ...hange the fuses The fuses are located in a fuse holder at the rear of the instrument Figure 26 Rear view fuse holder 1 Switch off the instrument and remove the line power cord from the electrical supply 2 Squeeze the two lugs at each side of the fuse holder and gently pull out Figure 27 Removing the fuse holder Fuse Holder Fuse Holder Artisan Technology Group Quality Instrumentation Guaranteed 888...

Page 104: ...be replaced at the same time 4 Replace the fuse holder Align the lug at the bottom of the fuse holder with the slot in the socket A click is heard as each lug snaps into place NOTE If you use the correct fuses but the instrument still does not work correctly or the fuses blow repeatedly contact your PerkinElmer office or representative Artisan Technology Group Quality Instrumentation Guaranteed 88...

Page 105: ...the U S call toll free 1 800 762 402 8 a m to 8 p m EST Your order will be shipped promptly usually within 24 hours If you are located outside of the U S call your local PerkinElmer sales office Accessories spares and other parts and information are available on line at essentials perkinelmer com Quantity Item Part Number 10 3 15 A slow blow fuse B0155573 10 6 3 A slow blow fuse B0155576 1 Deuteri...

Page 106: ...Analytical Notes 7 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 107: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 108: ... use stoppered cells to prevent this problem Samples not Governed by the Beer Lambert Law Quantitative analyses utilizing the absorption of spectral radiation are based on the Beer Lambert law which states that the absorption is proportional to the concentration of the analyte The law can be expressed in the form A ε c d Where A is absorbance ε is molar absorption coefficient c is molar concentrat...

Page 109: ...es advantage of the change in transmittance with time at a fixed wavelength For details refer to reference books covering this specific subject Photoactive Samples Some samples are known to be photoactive in that they fluoresce upon absorbing radiation Since a small portion of the fluorescent radiation will be measured by the detector a higher apparent transmittance will often result Samples are a...

Page 110: ...ce are not suitable as solvents for measurements in this region Water is virtually the only useful solvent below 195 nm but it must be freed from oxygen to attain best transmission Whenever you are going to use a solvent with unknown absorption characteristics scan its spectrum first to determine whether it is suitable The lower wavelength limits of a number of commonly used solvents are presented...

Page 111: ...Lambda 25 35 45 User s Guide 110 Figure 28 Lower Wavelength Limits of Solvent Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 112: ...Index Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 113: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 114: ...p 92 Use and Care of Cells 90 O Operating with Accessories 82 General 82 R Replacement Parts 105 Requirements for Operation with Accessories 83 S Safety information Electromagnetic Compatibility EMC 13 Safety Information in the Handbook 9 Safety Information 9 CSA Compliance 9 Electrical Protection 9 Electrical Safety 10 Environment 14 IEC 1010 Compliance 9 Solvent Properties 111 Startup and Shutdo...

Page 115: ...Lambda 25 35 45 User s Guide 114 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 116: ...l service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE INSPECTION Remotely inspect equipment before purchasing with our interactive website at www instraview com LOOKING FOR MORE INFORMATION Visit us on the web at www artisantg com for more information on ...

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