PerkinElmer Lambda 25, User Manual

Page: 69 / 116

Lambda 25, 35, 45 User’s Guide
68
3. In the UV WinLab Manual control dialog select transmission (%T) as ordinate
and click
Setup
.
4. Open the sample compartment cover.
5. Insert the cell with a low absorbing solvent into the sample cell holder.
6. Leave the reference cell holder empty.
7. Make horizontal fine alignment to the sample cell holder (locking screws and
milled posts loosened) to obtain the highest possible transmittance reading on
the display (close sample compartment cover while measuring transmittance).
8. Make vertical fine adjustment using the vertical adjustment screw again to
obtain the highest possible reading (close sample compartment cover while
measuring transmittance).
9. When you are satisfied with the alignment, tighten the milled posts and the
locking screws on the cell holder.
10. Insert the matching cell with the same low absorbing solvent into the reference
cell holder.
The first cell remains in the sample cell holder.
11. Repeat steps 5 to 9 with the reference cell holder, but this time obtain the
lowest possible transmittance reading on the display.
This completes the fine alignment procedure.
NOTE: When the cell holder has been aligned once, you can take it out and reinstall it
without aligning it again.
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