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PerkinElmer EnVision, Instrument Manual

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PerkinElmer EnVision Instrument Manual Download Page 82

Specifications 

72 

 
 

Measurement modes  

•

 

Fluorescence intensity from top  

•

 

Fluorescence intensity from top and bottom  

•

 

Time-resolved fluorescence from top  

•

 

Time-resolved fluorescence from top and bottom  

•

 

Multiple window TRF  

•

 

Homogenous TRF (LANCE, HTRF, LanthaScreen)  

•

 

Fluorescence polarization  

•

 

Absorbance  

•

 

Luminescence 

•

 

Ultra Sensitive Luminescence  

•

 

Dual emission  

•

 

AlphaScreen or HTS AlphaScreen  

•

 

Adjustable measurement height 

•

 

Dispensing 

 

Plate shaking  

Three plate shaking modes are available: linear, orbital and double orbital. 
Three speed levels can be selected and the amplitude of the movement is 
adjustable.  

Scanning  

Scanning of wells (several measuring points within a well) are available for 
all technologies.  

Kinetics  

Repeated measurements or operations can be defined by plate or well.  

Monochromator option 

Two double monochromators are located inside the instrument casing. Light 
fibers are used to guide the light to and from them. The excitation light from 
the lamp is directed through the excitation double monochromator into the 
sample. The emission light from the sample is directed through the emission 
double monochromator to the detector.  

Applications supported  

•

 

Photometric application range 230 – 1000 nm 

•

 

Photometric spectrum scan 230 – 1000 nm 

•

 

Fluorescence intensity application range 230 - 850 nm 

•

 

Fluorescence intensity excitation & emission spectrum scan 
measurement 230 - 850 nm 

Summary of Contents for EnVision

Page 1: ...EnVision Multilabel Reader 2104 9030 02 March 2008 INSTRUMENT MANUAL ...

Page 2: ......

Page 3: ...ultilabel Reader Valid for instruments with software version 1 12 PerkinElmer Life and Analytical Sciences Wallac Oy P O Box 10 FIN 20101 Turku Finland Tel 358 2 2678111 Fax 358 2 2678 357 Website www perkinelmer com ...

Page 4: ......

Page 5: ...ufacturer s recommendations Installation and service must be performed by personnel properly trained and authorized by PerkinElmer Life and Analytical Sciences Failure to follow these instructions may invalidate your warranty and or impair the safe functioning of your equipment ...

Page 6: ......

Page 7: ...rbance 240 950 nm 9 AlphaScreen HTS AlphaScreen 520 620 nm 9 Mechanical design overview 10 Temperature control 11 Plate types 11 Plate height sensor 11 Alternative plate loading modes 11 Flexible stacker 11 Robotic loading 11 Optical system 12 Luminescence mode 13 Ultra Sensitive luminescence mode 14 Fluorescence Intensity 14 TR fluorescence mode 17 Fluorescence polarization mode 21 Absorbance mod...

Page 8: ... Reference manual 37 Administrator manual 37 Quick start guide 37 On line help 37 Routine maintenance 37 Warnings 38 Report on laser safety EnVision with the TRF laser option 39 General 39 Outer covers and openings of EnVision 39 Safety switches and lid locking 41 Laser beam path 44 Detaching the optical fiber 44 Additional safety features of the instrument 44 Laser warning labels 45 Contact addre...

Page 9: ... Kinetics 72 Monochromator option 72 Applications supported 72 Photometric performance with monochromator 73 Fluorescence intensity performance with monochromators 73 Other optional modules 73 Measurement time and performance 73 DECLARATION OF CONFORMITY FOR CE MARKING 79 WEEE Instructions for PerkinElmer Products 81 Glossary 85 Installation 97 Environment 97 1 Unpacking instructions 97 2 Installa...

Page 10: ...gistered trademarks of PerkinElmer Inc Windows Windows XP and Windows Vista are registered trademarks of Microsoft Corp in the U S and other countries Pentium is a registered trademark of Intel Corporation LanthaScreen is a trademark of Invitrogen Inc HTRF is a registered trademark of Cisbio International ...

Page 11: ...1 Chapter 1 Introduction ...

Page 12: ...2 ...

Page 13: ... model allows AlphaScreen measurements to be made HTS AlphaScreen allows faster throughput than standard AlphaScreen A model with an external laser provides enhanced time resolved fluorescence capabilities for TRF LANCE and HTRF EnVision is a very compact small footprint bench top unit with features such as shaking reading from above or below and scanning Also available are a dispenser with up to ...

Page 14: ...If in the future you wish to add new features this is also possible Conventions used Buttons or other software items to be clicked with the mouse are in bold text e g File Items in the main Navigation Tree are in bold italic e g Protocols Buttons on the instrument that need to be pressed are in block letters e g START Note The term AlphaScreen refers to both standard AlphaScreen and HTS AlphaScree...

Page 15: ...5 Chapter 2 Functional description ...

Page 16: ...6 ...

Page 17: ...spase assays for example EnVision reads very rapidly using a two detector system The temperature control option enables enzyme applications to be run in stabilized conditions The instrument s kinetics facility and for example its flexible stacker system option allow you to work fast and effectively Time resolved fluorometry DELFIA and LANCE assays are based on time resolved fluorescence methods us...

Page 18: ... 384 shallow well microplate coated with scintillant emitting in the red range of the spectrum thus eliminating most of the interference from colored compounds AlphaScreen assays AlphaScreen is an ideal tool for screening a broad range of targets The technology provides an easy and reliable means to determine the effect of compounds on biomolecular interactions and activities AlphaScreen offers th...

Page 19: ...ith the monochromator option 2 double monochromators support flexible wavelength selection Alternatively filters may still be chosen to provide excellent speed and performance Fluorescence polarization 450 750 nm Simultaneous dual emission measurements Luminescence Simultaneous dual emission measurements Glow luminescence measurements Ultra Sensitive luminescence Radioluminescence measurements wit...

Page 20: ...veyor 30 Barcode reader holder 31 Barcode reader 32 Plate carrier 33 Z movement module 34 Lifting module stacker 35 Magazine for 20 plates Note With Ultra Sensitive Luminescence there is an additional detector for this measurement This is located next to the other detector s The detector used for Ultra Sensitive Luminescence is also used for HTS AlphaScreen if that is installed Note With AlphaScre...

Page 21: ...magazines Rods come up from the table and the plate is loaded onto them Flexible stacker The stacker provides a semi automated plate loading option Each stacker carries up to 20 or 50 plates at a time The plate stacker includes robust magazines which are easy to lift into place and remove With an easy one hand operation you place and remove the loaded magazine Measurement is started with a single ...

Page 22: ...le volume The excitation optical system also employs two lenses They are coupled with a short length of quartz fiber bundle The use of the fiber bundle allows the optimal location of filter slides so that you can access them easily Another important advantage of using the fiber bundle is to ensure delivery of a confined and stable circular spot into the sample volume Figure 4 Overall view of the o...

Page 23: ...le optics and directed through the aperture of the luminescence mirror module 2100 404 This aperture effectively decreases the crosstalk from adjacent wells The filter optics guides the light from the aperture into the photocathode of the side window photomultiplier tube The single photon counting system counts the number of photons for a pre selected time period and the counter is then read by th...

Page 24: ...high voltage of the photomultiplier i e the gain is a parameter that can be set by the user Changing the parameter value from 1 to 1024 changes the gain from 50000 to about 10 million i e over two orders of magnitude The basic measurement needs only one flash but for higher accuracy it could be useful to use more flashes The integrated analog signal from the photomultiplier and the reference photo...

Page 25: ...hroic mirror is about three times higher than with a general mirror module Figure 8 Excitation from above Reading from above Figure 9 Excitation from below Reading from below Figure 10 Excitation from above Dual reading from above ...

Page 26: ...ating The grating separates the incident polychromatic beam into its constituent wavelength components sending each wavelength into a different direction so that a narrow band of wavelengths can be collected Double monochromators contain two diffraction gratings The use of monochromators provides the benefit that wavelength can be selected steplessly through the workstation software Although monoc...

Page 27: ...from the same excitation energy Most TRF measurements are done in epi mode i e excitation from above and reading from above or excitation from below and reading from below or in through mode The general mirror module 2100 4010 with 50 50 beamsplitter mirror can be used for all labels independent of the excitation and emission wavelengths For dual detection the dual general mirror module 2100 4050 ...

Page 28: ...Functional description 18 Figure 15 Excitation from below Reading from below Figure 16 Excitation from below Dual reading from below Figure 17 Excitation from below Reading from above ...

Page 29: ... Note A dual mirror is needed whenever the second detector is used even if the reading is done from opposite sides of the plate The mirror in the light path to the second detector is the one that must be dual as noted in the figures Figure 19 Excitation from above Reading from below ...

Page 30: ...ve Dual reading from below Figure 21 Excitation from above Reading from above and reading from below second detector Bottom mirror must be dual Figure 22 Excitation from above Reading from above second detector and reading from below Top mirror must be dual ...

Page 31: ...ical module The excitation light is polarized by a polarizing filter in the optical module The filter polarizes the light in the S plane The integrated photon counts from the photomultiplier and the integrated analog signal from the reference photodiode are always read after every flash For one measurement one or more flashes these readings are summed not averaged The reference signal is then comp...

Page 32: ...ents the two planes will be measured sequentially by changing the emission filter The final result is calculated by combining these measurements using the formula Polarization mP 1000 S G P S G P where S and P are the measured results with the S and P emission filters respectively G is a factor to correct for the effect of emission filter transmission variations differences in the emission light p...

Page 33: ... where the intensity of the light is measured using a reference photodiode The light is then directed through the bottom of the plate and focused into the sample The focal plane is the same as for FI TRF and FP measurements The intensity of the light is first measured without any sample and then the samples in one plate are measured The light intensity is measured by a photodiode placed at an opti...

Page 34: ...onochromator option light passing into the sample comes from the excitation double monochromator For users who do not wish to perform fluorescence intensity measurements the single monochromator option may be chosen rather than the quad monochromator Figure 28 Absorbance measurement using either the quad or single monochromator option ...

Page 35: ... bead which emit fluorescence light in the range 520 to 620 nm The long half life of the signal permits the measurement to be time resolved to reduce the contribution of background fluorescence The fact that the excitation wavelength is longer than the emission further reduces the background as does the fact that wavelength itself is long In the case of AlphaScreen the emission light pathway invol...

Page 36: ...a factor that must also be taken into account There are three components bleaching caused by the excitation of adjacent samples afterglow from a previously excited adjacent sample and glow from an adjacent well at the time of measurement A crosstalk correction wizard allows the amount of the crosstalk to be measured and the software uses this as a correction factor in the calculation of the final ...

Page 37: ... as time resolved fluorescence and fluorescence polarization For every measurement type you can select the number of flashes used To ensure both the long term and short term stability of measurements the excitation energy is monitored after every flash via a beamsplitter and reference photodiode In the case of AlphaScreen a laser emitting light at wavelength of 680 nm is used In the case of enhanc...

Page 38: ...oic beamsplitter for AlphaScreen a bias mirror module for use with the second light source All application specific mirror modules are barcoded For reading from above the multiple mirror module can hold up to four mirror modules Any of the mirror modules can also be in the head for reading from below but the barcode is not then read automatically The mirror modules used for dual channel measuremen...

Page 39: ...checks that the selected aperture is in place and gives a warning message if it is not You can still continue despite this warning if you choose to A shutter is available to protect the additional detector from dust when it is not being used e g for several days or longer This shutter must be used instead of an aperture when standard AlphaScreen measurements are made because the other apertures pr...

Page 40: ...kground and very high signal to background ratio A laser and AlphaScreen coated bead technology are used Large wavelength regions Large wavelength ranges are needed in DNA and protein qualification assays EnVision supports wavelengths from 240 nm to 850 nm Luminescence mode When a luminescence sample is measured no excitation components are used Minimized crosstalk in luminescence measurements The...

Page 41: ...on is suited Three modes are available linear orbital and double orbital The duration speed and amplitude of shaking can all be defined by the user Scanning Cell applications require several reading points in one well Due to the heterogeneous spread of cells in a well it is important to scan the complete area of the cell culture This can be done with EnVision by specifying the shape of the scan ar...

Page 42: ...he system A tip mount has three tip positions of which two can be used at a time These positions are named from left to right Post Real time and Pre The Real time position is the measurement position Post is the position the well moves to after the measurement position Pre is the position it is in before moving to the measurement position Pump connections are as shown in the table Tip configuratio...

Page 43: ...the instrument Barcodes Your instrument uses the following barcodes Filter mirror barcodes All filter and mirror modules are barcoded this makes their use much easier Plate barcodes The barcode reader allows you to load barcode labeled plates which are identified by the barcode reader Codabar Code39 Interleaved 2 of 5 and Code 128 barcodes can be read Tip mount barcodes Each tip mount is barcoded ...

Page 44: ...er instruments is easy to achieve due to the COM interface and the Windows environment Relation to other systems EnVision can be used independently as a stand alone manually loaded system EnVision can be used as a robot controlled subsystem in an automated HTS laboratory The data generated by EnVision can be transferred via the Windows operating system and a computer network to other systems Diffe...

Page 45: ...35 Chapter 3 Information about user instructions and warnings ...

Page 46: ...36 ...

Page 47: ...his instrument manual It describes the Enhanced Security software It is intended for users with Administrator rights Quick start guide This is a plasticized card that gives an overview of the steps involved in operating EnVision On line help This is supplied with the software and can be accessed by clicking Help or F1 in any EnVision window This help gives detailed information about all features o...

Page 48: ...e instrument Warning Disconnect supply before servicing On the back of the instrument Warning CLASS 1 LASER PRODUCT EN 60825 1 A1 2002 A2 2001 Inside the instrument Caution To avoid the risk of electric shock or exposure to ultraviolet light do not unscrew any parts Note See also the additional warnings associated with the optional TRF LASER as shown on the following pages Safety symbols used Powe...

Page 49: ...trument is a class 1 laser product This is possible because EnVision has adequate laser shielding which prevents the user being exposed to the laser radiation Outer covers and openings of EnVision The case assembly lid loading door and side hatch of the instrument form a light tight system Light tightness is essential not only for laser safety but also for the functional performance of the instrum...

Page 50: ...t the end wall of the casing The tip of the arrow shows the approximate spot where the laser beam would hit because of this kind of error Furthermore the diffuse reflection from the inner surface of the protective casing does not cause immediate danger to the user according to class 3B not even if the user looks straight at the illuminated spot Picture 2 also shows the optical fiber of the laser t...

Page 51: ... designed so that two solenoids will lock the lid before the measurement is started However there is no specific control to ensure that the locking mechanism functions properly Therefore besides the locking mechanism additional protection is needed The purpose of the locking mechanism is mainly to prevent the user from opening the lid during measurement and destroying the measuring results Laser l...

Page 52: ...n such a case the laser operation is stopped and the laser beam shutter is closed Thus it is possible to use the laser only when the magnetic switch detects that all openings are closed The longest possible distance for the magnetic switches to work is approximately 5 mm and the moving distance of the openings before actual opening is much longer than this Hence even the slightest gap in the door ...

Page 53: ...Information about user instructions and warnings 43 Magnetic sensor Magnet Picture 4 Magnetic switch on the loading door Magnetic sensor Side hatch magnets Picture 5 Magnetic switch on the side hatch ...

Page 54: ... laser and optical fiber as well as the cover plate of the electronics compartment are fastened with screws Therefore it is impossible to reach the laser or its fiber connectors without tools Additional safety features of the instrument To ensure laser safety the device contains two special features These are the automatic closing of the beam shutter and the measuring of laser pulse light before t...

Page 55: ...n is stopped right after the first pulse and an error message is displayed Laser warning labels According to standard IEC 60825 1 a class 3B laser must contain a warning label with the following text LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT Standard IEC 60825 1 also states that all couplings and protective structures must contain a warning label if there is a risk of being exp...

Page 56: ...Informations about user instructions and warnings 46 Picture 8 Warning label affixed on the protective casing of the laser Picture 9 Laser class label on the cover of the electronics compartment ...

Page 57: ...Information about user instructions and warnings 47 Picture 10 Laser warning label inside the instrument ...

Page 58: ...tham Massachusetts 02451 USA 800 551 2121 European Headquarters PerkinElmer Life and Analytical Sciences Imperiastraat 8 B 1930 Zaventem Belgium Tel 32 2 717 7911 Manufacturer PerkinElmer Life and Analytical Sciences Wallac Oy P O Box 10 FIN 20101 Turku Finland Tel 358 2 2678111 Fax 358 2 2678 357 Email info perkinelmer com Website www perkinelmer com ...

Page 59: ...49 Chapter 4 Routine maintenance ...

Page 60: ...50 ...

Page 61: ...signal generation component clean the area where the spillage occurred in a similar way to that described above except that you first use Enhancement Solution to remove e g the europium then a mild detergent or alcohol and finally distilled water Let the area dry before starting to use the instrument Filters should be free of finger prints Fingerprints on filters should be removed with 99 8 vol Al...

Page 62: ...ust tissue with pure ethanol 5 Put your hand into the instrument through the plate door of the instrument and clean the lens see picture below in which the horizontal head is shown vertically The lens is not directly visible but can be cleaned from the outside With a small mirror it is possible to check that the lens look clean With plate turned around remove tip mount Clean lens through plate doo...

Page 63: ...need to remove the magazines and upper part of the stacker the magazine table 1 Lift off the magazines 2 Pull forward the magazine table 3 Lift off the magazine table Note If the rods of the stacker are up click the Reset stacker button in Reader settings Stacker to get the magazine table out ...

Page 64: ...hanging filter slides 1 Lift the instrument lid 2 You will see the ends of the excitation and emission filter slides directly in front of you Pull out the slide you want to get access to 3 Add remove or exchange filter modules as needed 4 The filter module snaps into place in the filter slide ...

Page 65: ...suring head body where the filter slides are also located Up to four mirror modules are mounted on a rotatable mirror module changer You can turn this to get access to the mirror module you want There is a metal wedge after which comes mirror modules 1 2 3 and 4 in clockwise order 2 Use the special tool to remove the mirror module The sprung metal piece in the center of the tool must be pressed do...

Page 66: ...ok the tool 6 Close the lid when you have finished 7 The instrument will read the barcodes on the mirror modules and update the list of mirrors in the software Changing the bottom mirror Note This operation has to be performed by touch and not by sight so the pictures do not show the components when they are actually installed inside the instrument 1 Release the white cover on the right side of th...

Page 67: ...ned screw The figure shows the screw in the bottom mirror module holder You need to find this screw with your fingers and turn it anti clockwise to release it 4 Carefully withdraw the bottom mirror module holder from its position inside the bottom measuring head body Then holding it in your hand bring you hand out through the hole in the side of the instrument ...

Page 68: ... information in the software When you replace the cover a series of prompts appear on the user interface telling you to give this information This is described in the Reference manual in the Mirrors chapter Changing the aperture If you are going to make measurements using one of the options Ultra Sensitive Luminescence or HTS AlphaScreen you must use the correct aperture for the plate type you are...

Page 69: ...instruments will have one of the following types of head to secure the aperture slide 3 Unscrew and remove the screw head left pictures or twist anti clockwise and pull right Use your fingers no tool is necessary 4 Pull out the aperture ...

Page 70: ...the instrument lid Note If errors occur with the aperture you should check that the aperture is correctly installed There is a possibility that the aperture block did not go far enough into its place when the screw was tightened Changing a tip mount Open the instrument lid and the dispenser unit lid To remove the existing tip mount first loosen the brass screw and then pull out the tip mount ...

Page 71: ...licone connectors Lift the tip mount away from the instrument Take the new tip mount Make sure it has the tip configuration you want Carefully push each tube from the dispenser into the silicone connectors at the end of the tubes coming from the tip mount ...

Page 72: ...connector because this affects the output tube volume Note The tube from Tip 1 which is to be connected to the tube from Pump 1 has a sock shrunk onto it to help you distinguish it from the tube from Tip 2 Connections should be as in the table Tip configuration Pump1 Pump2 Real time Real time Not used Pre Pre Not used Real time and Pre Real time Pre Pre and Post Pre Post ...

Page 73: ...f the dispenser so the silicone connector is pulled as close as possible to the dispenser connection piece Arrange the tubing so that it rests on top of the support piece This prevents the tubing getting in the way of the mirror module changer Close the instrument lid Make sure that the correct liquid reservoir and liquid are installed for each pump and that the aspiration tubes go to the correct ...

Page 74: ...tion in the user interface to prepare and use the tip mount See the Tip Mounts chapter in the Reference manual for more information about selecting the correct tip mount See the Dispenser Control chapter in the User manual for dispenser operations ...

Page 75: ...65 Chapter 5 Specifications ...

Page 76: ...66 ...

Page 77: ...conditions in addition or in excess to those stated in the operating conditions Altitude up to 2000 m Temperature 5 C to 40 C Relative humidity Maximum 80 at 31 C decreasing linearly to 50 at 40 C Mains supply fluctuations 10 Installation category overvoltage category II according to IEC 664 1 Note 1 Pollution degree 2 according to IEC 664 1 Note 2 Note Installation category overvoltage category d...

Page 78: ...tibility 73 23 EEC as amended by 93 68 EEC Low Voltage Directive Performance specifications used to verify conformance to the Directives above EN 61326 class B Requirements IEC 61010 1 1990 A1 1992 A2 1995 Environmental conditions Operating conditions 15 C to 35 C Relative humidity 80 maximum at 31 C decreasing linearly to 65 at 35 C indoor use Operating conditions for AlphaScreen 20 C to 25 C Rel...

Page 79: ...der Windows XP on a Pentium computer 1 7 GHz minimum 512 MB memory with 30 GB available hard disk space or Windows Vista on a Intel Core 2 Duo E4300 1 8 GHz 800 MHz 2MB minimum 1GB memory with 80 GB available hard disk space The computer is equipped with a CD ROM an Ethernet card and a 24 bit color display with minimum resolution of 1024 x 768 pixels Plates 1 to 1536 well plates are compatible wit...

Page 80: ...e it for the holders to fit in There must also be an adequate amount of ledge sticking out for the holders to get underneath so that they can support the plate and the rest of the plates stacked above it Note A plate locking mechanism ensures that a plate can be removed from a stack even if there is a tendency for plates to stick together due to e g tape on top of a plate Note Plates with heights ...

Page 81: ...ors Filters Changeable excitation filter slide provided with 8 filter positions Ø15 0 mm Changeable emission filter slide provided with 8 filter positions Ø15 0 mm Barcoded filters Apertures Three apertures are available for 1536 384 and 96 well plates respectively These are for Ultra Sensitive luminescence or HTS AlphaScreen measurements A shutter is available to protect the Ultra Sensitive lumin...

Page 82: ...djustable Scanning Scanning of wells several measuring points within a well are available for all technologies Kinetics Repeated measurements or operations can be defined by plate or well Monochromator option Two double monochromators are located inside the instrument casing Light fibers are used to guide the light to and from them The excitation light from the lamp is directed through the excitat...

Page 83: ...Other optional modules Stacker Barcode Readers 2nd Detector Ultra Sensitive Luminescence and HTS AlphaScreen Detector AlphaScreen or HTS AlphaScreen laser light source and plate cooler unit TRF laser 337 nm nitrogen laser Control unit Temperature control Dispenser Measurement time and performance Measurement times Technology Number of flashes Time for 96 wells Time for 384 wells Time for 1536 well...

Page 84: ...nsity monochromator Detection limit 96 plate 200 µL 10 pM 2 fmol well Fluorescein 100 flashes from top Fluorescence intensity monochromator Detection limit 384 plate 50 µL 80 pM 4 fmol well Fluorescein 100 flashes from top Fluorescence intensity monochromator Bandwidth Excitation Emission 8nm Fluorescence polarization Detection limit 96 plate 200 µL 3 mP 1nM Fluorescein 100 flashes Fluorescence po...

Page 85: ...racy 2 OD 2 405 nm Precision 2 OD 0 1 405 nm Absorbance Filter Monochromator Measurement range 1536 plate 7 5 µL 0 3 OD 405 nm Absorbance Monochromator Bandwidth 8 nm Absorbance Monochromator Wavelength accuracy 2 0 nm Absorbance Monochromator Wavelength precision 0 2 nm Luminescence Lower limit of detection 96 plate 200 µL 75 pM ATP 1 second Ultra Sensitive Luminescence Lower limit of detection 3...

Page 86: ...Specifications 76 ...

Page 87: ...77 Chapter 6 Quality information ...

Page 88: ...78 ...

Page 89: ...040001 to which this declaration relates is in conformity with the following standard s or other normative document s Title and or number and date of issue of the standard s or other normative document s EN 61326 1998 EN 61010 1 2001 if applicable following the provisions of the following directives Electromagnetic compatibility EMC 89 336 EEC Low voltage LV 72 23 EEC Date and place of issue 5 Dec...

Page 90: ...80 ...

Page 91: ...ustomer s environment Requirements for waste collection reuse recycling and recovery programs vary by regulatory authority at your location Contact your local responsible body e g your laboratory manager or authorized representative for information regarding applicable disposal regulations Contact PerkinElmer at the web site listed below for information specific to PerkinElmer products Web address...

Page 92: ...82 ...

Page 93: ...83 Chapter 7 Glossary ...

Page 94: ...84 ...

Page 95: ...andard AlphaScreen and HTS AlphaScreen options when referring to features that are common to both alphascreen option separate option including a laser light guide mirror module filter and plate cooler for AlphaScreen measurements Note AlphaScreen is a measurement technology based on the use of coated beads Donor beads are excited by laser light Energy is transferred to bound acceptor beads which t...

Page 96: ...e aperture offset from the center position biased excitation aperture whereas mirror modules used with light source 1 have the aperture in the center This bias mirror module comes in a single mirror module version and a dual mirror module version bottom excitation cable optical fiber to guide the excitation light from the light director assembly into the inlet aperture of the mirror in the bottom ...

Page 97: ...ion and analogue mode dichroic mirror beamsplitter that permeates reflects light of a certain wavelength range mirror used to selectively transmit light according to its wavelength diffraction grating a device for dispersing light used as the basis for a monochromator dispenser unit device for dispensing to a microtitration plate in EnVision one or two pumps can be installed A tip mount is used to...

Page 98: ...er optical component letting only a certain bandwidth range of light pass through filter frame support onto which the filter block is mounted filter module assembly consisting of the filter frame the filter a barcode a polarizer and a possible aperture plate filter slide assembly consisting of the filter holder and the filter blocks enabling automatic filter switching Note The maximum number of fi...

Page 99: ...th instrument case assembly outer cover protecting the instrument laser a laser emitting light at a wavelength of 680 nm and used to excite samples in AlphaScreen and HTS AlphaScreen measurements lens assembly component that contains one plano convex lens and a lens holder with locking rings Note The instrument has four different lens assemblies with different lens features and at different locati...

Page 100: ...light tight sliding door and the related mechanism Note The approx dimensions height and width of the door are 35mm 320mm luminescence aperture slide user changeable plate specific slide with suitable aperture to mask luminescence emission magazine detachable part of the stacker to hold the sample plates measurement height changer module to adjust the height of the measuring heads from the point o...

Page 101: ... from the light director assembly directly to the photometric optics photometric detector part located above the sample for signal measurement it contains a photodiode and a movement motor to ensure that the photodiode s position is optimal from the plate in photometric measurements Note The photometric detector is mounted in the mirror module photometric optics assembly to reconstruct the emissio...

Page 102: ... positioned above and below the measuring position to maintain the temperature of assay plates with samples requiring above ambient temperature tip mount comprises one or two tips and their mounting Part of the dispenser there are three tip positions any two of which can be used top measuring head measuring head positioned above the x y conveyor top measuring head body body section of the top meas...

Page 103: ...ed for ultra sensitive luminescence and HTS AlphaScreen measurements waste container small container in EnVision for dispensed waste liquid This is automatically emptied by a waste pump using the aspiration tip x y conveyor mechanism that moves the plate carrier in the horizontal plane between different functional positions including from either the stacker or the manual loading position to the po...

Page 104: ......

Page 105: ...95 Chapter 8 Installation ...

Page 106: ...96 ...

Page 107: ...ge are anticipated a mains stabilizer may be necessary Note Make sure that when the instrument is at its place of operation it is exactly level This is important to ensure proper function of the stacker 1 Unpacking instructions Follow the instructions on the document attached on the instrument 2 Installation of options If the instrument has been ordered with stacker dispenser or TRF LASER follow t...

Page 108: ... the PC and the instrument or to the dispenser if installed Note Do not connect the cable to the RS232 port of the PC Connect the instrument and computer to the mains Switch the computer on and log in to the EnVision user See documentation with the computer for User Name and password Start the service program and run the performance test see the Service support folder for detailed information ...

Page 109: ...uminescence 30 HTS AlphaScreen 31 Crosstalk correction Ultra Sensitive luminescence 30 D Delay 31 DELFIA assays 7 Detector 28 71 Second 28 Detector gain 14 Diffraction grating 16 Dimensions 69 Direct Double Optics 12 Dispenser 3 31 71 dual dispensing 31 Modes of operation 32 Real time measurement 31 Dual ratio measurements 30 E Enhanced height sensor 59 Enhanced luminescence Apertures 29 71 Enviro...

Page 110: ...r Bottom 57 Dual 19 Mirror module Changing 55 Tool 56 Mirror modules 28 Dual 28 Mirrors 71 Monochromator 3 9 16 24 72 73 91 Multiple window TRF 29 N Navigation tree 4 O Online Help 37 Output 3 Overview 3 P PC configuration 33 69 Plate height sensor 11 Plate loading modes 11 Plate shaking 72 Plates 11 69 Power requirements 68 Printer 33 Printer installation 98 Q Quick start guide 37 R Reading Above...

Page 111: ...ce mode 17 Dual measurement 17 Fast dual 29 Multiple window 29 Time resolved fluorometry 7 Tip mount 32 Changing 60 Installation 63 98 Barcode 33 TRF laser mode 26 U User instructions 37 User interface 34 User manual 37 W Warnings 38 Waste container 33 Wavelength Large range 30 Workstation interface 34 ...

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