PerkinElmer EnVision, Instrument Manual

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Introduction
3
Introduction
The EnVision
™
microplate reader from PerkinElmer Life and Analytical
Sciences is a complete platform for quantitative detection of light-emitting
or light absorbing markers in research and drug discovery applications. It is
suitable for measurement of fast or glow luminescence, fluorescence
intensity, fluorescence polarization ([FP]
2
™), high-sensitivity time-resolved
fluorometry (DELFIA
®
) and homogeneous time-resolved fluorometry
(LANCE
™
). It can also be used to measure absorbance.
EnVision may be equipped with a monochromator option. In measuring
absorbance and fluorescence intensity, monochromators provide an
alternative to the use of specific waveband filters, and allow greater
flexibility. The quad monochromator option includes two double
monochromators. A single monochromator option is also available for
absorbance assays.
Very high sensitivity measurements can be made with the Ultra Sensitive
luminescence option.
A laser-equipped model allows AlphaScreen measurements to be made.
HTS AlphaScreen allows faster throughput than standard AlphaScreen.
A model with an external laser provides enhanced time-resolved
fluorescence capabilities for TRF, LANCE and HTRF.
EnVision is a very compact, small footprint bench top unit with features
such as shaking, reading from above or below and scanning.
Also available are a dispenser with up to two pumps and temperature
control of the instrument chamber.
The single point reading technology with extremely fast and accurate
mechanical movement allows the reading of high-density plates as well as
use of a wide variety of plates or sample matrix formats. It accepts all types
of microtitration plate. It measures 96-well plates in less than 20 seconds
and 1536-well formats in under one minute. Plates can be loaded manually
or plates can be loaded using 20 or 50-plate free access magazines which fit
in a stacker.
Alternatively, robot-friendly EnVision is easy to connect to laboratory
automation.
The software is a 32-bit application running under Windows XP
®
or
Windows Vista
®
. Output can be to a file on the PC and/or to a laser printer
or on the network.