PerkinElmer EnVision, Instrument Manual

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Functional description
8
Cellular assays
Reading from below, scanning and kinetics are some of the features that
make Wallac EnVision the ideal tool for cellular assays such as cAMP,
Ca
2
+ or any ADME/tox assays. The temperature control option further
enhances these possibilities.
Genotyping assays
A feature of single nucleotide polymorphisms (SNPs) research is the need
for fast results. With a two-detector configuration, stacker, plate barcode
reader, as well as factory set protocols to cover all labels, plates and filters,
EnVision provides a complete facility for fast detection of SNPs.
Image FlashPlate™ assays
Ultra Sensitive Luminescence enables measurement of Image FlashPlate
assays. This assay measures radioactive samples. The radioactivity is
detected using energy transfer via a scintillant and a europium chelate
(emission at 615 nm). The Image FlashPlate is a 384 shallow well
microplate, coated with scintillant emitting in the red range of the spectrum,
thus eliminating most of the interference from colored compounds.
AlphaScreen assays
AlphaScreen is an ideal tool for screening a broad range of targets. The
technology provides an easy and reliable means to determine the effect of
compounds on biomolecular interactions and activities. AlphaScreen offers
the possibility to assay many biological interactions including low affinity
interactions as well as enzymes, receptor-ligand interactions, second
messenger levels, DNA, RNA, proteins, peptides, sugars and small
molecules).
Assays with dispensing
The dispenser option enables applications that require immediate
measurement after the activation step. The dispenser is needed, for example,
in assays such as calcium ion-channel. The signal is measured
simultaneously during the dispensing step followed by a 30 second kinetic
measurement. In a flash luminescence application the substrate is dispensed
into the well, the plate is mixed for 2 seconds and the signal is registered.