Instruction Manual
Thermal cycler
peqSTAR 2X Double block thermal cycler peqSTAR 96X, 384X and in situ X thermal cycler
v1212E
Creating the future together.
Page 1: ...Instruction Manual Thermal cycler peqSTAR 2X Double block thermal cycler peqSTAR 96X 384X and in situ X thermal cycler v1212E Creating the future together ...
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Page 3: ... 5 7 2 3 Stop 5 7 2 4 Pause 5 7 2 5 Info button 6 7 2 6 Lid lock unlock 6 7 2 7 Select program 6 7 3 Select Program dialogue 6 7 4 Info Button 6 7 4 1 Steps 6 7 4 2 Program Options 7 7 4 3 Print 7 7 5 Lid lock unlock 7 7 6 Starting a program 7 7 7 A program is running 8 7 7 1 General Pause and Stop button 8 7 7 2 Pause button 8 7 7 3 Stop button 8 7 7 4 Status Display 8 7 7 5 Program window 9 7 7 ...
Page 4: ...cle 21 9 3 8 Start Cycle Manual cycle beginning 21 9 3 9 Close Cycle Manual cycle ending 21 9 3 10 Pause 21 9 3 11 Store 21 10 Diagnostics 22 10 1 Operation of the Diagnostics dialogue 22 10 2 General view 23 10 2 1 Zones 23 10 2 2 Single view 23 10 2 3 Automatic scrolling active 23 10 2 4 Automatic scrolling inactive 23 11 GLPs dialogue 24 11 1 Displaying a GLP report 24 11 2 Operating elements o...
Page 5: ...7 12 11 4 Zigbee Configure 37 12 11 5 Zigbee Connect 38 12 11 6 Zigbee Disconnect 39 12 12 USB Recovery 39 12 13 Service 39 13 Maintenance repair of the Thermal cycler 40 13 1 Cleaning maintenance and repair of the thermal cycler 40 13 2 Test of the overtemperature protection 40 13 3 Temperature check up 40 13 4 Calibration 40 14 Warranty 40 15 Disposal Instruction 41 16 Technical data 41 16 1 Gen...
Page 6: ...ally in the network e g for other thermal cyclers Transfer of data can be done by using commercially available USB memory sticks enabling a nearly unlimited saving space for programs and GLP reports which can be printed using a printer connected to the thermal cycler via the network Notice Notice Notice Notice The USB ports support only standard cable mice and standard USB sticks The maximum accep...
Page 7: ...hermal cycler onto a solid surface The device should not be exposed to direct sunlight There should be enough space to make sure that the ventilator grills on the front and back are not covered and sufficient ventilation is provided The accessibility of the mains switch must be assured and not hindered There should be a distance of at least 25 cm 10 inch to the wall or the next device Two thermal ...
Page 8: ...l software of the thermal cycler except for some system settings If the user has to enter numbers or text the corresponding field has to be touched on the touch screen or clicked with the mouse An appropriate keyboard will appear on the screen according to the expected entry All functions of the thermal cycler will be served by operating elements explained in the following chapters An operating el...
Page 9: ...rams This dialogue is used for creating editing and organizing PCR protocols see chapter 9 6 3 Diagnostics This dialogue is used for monitoring the actual temperature profile of the thermoplate Also the temperature of the heated lid and the temperature of the cycler interior are displayed see chapter 10 6 4 GLPs This dialogue is used for displaying and organizing GLP reports created by the thermal...
Page 10: ...e incubation mode can be activated In this mode block and lid temperatures can be adjusted manually see chapter 8 7 2 2 Start Use this button to start a PCR protocol 7 2 3 Stop Use this button to stop a PCR protocol which is in progress 7 2 4 Pause Use this button to pause a PCR protocol which is in progress Program window Block selection left right only for peqSTAR 2X systems Direct help List vie...
Page 11: ...sing this button If a program is selected the adjoining window appears If a program is marked the i button appears By Open the chosen program will be loaded in the run dialogue Further information on this dialogue can be found at chapter 9 1 1 7 4 Info Button Using this button a new window will open which shows detailed information of the chosen program If wanted this information could be printed ...
Page 12: ...ocal network administrator for the required settings If the path for the printer is already entered simply click for printing Using the button a test printing will be performed to check the correct settings 7 5 Lid lock unlock Use this button to lock or unlock the lid For systems with HPL lids High Pressure Lid there is the option to define the lid pressure in an opening dialogue 7 6 Starting a pr...
Page 13: ...ing the break the status Paused is shown 7 7 3 Stop button If this button is pressed after starting a program the adjoining security query appears By confirming the security query with Yes the program will be stopped 7 7 4 Status Display In this window the following information on the status is shown during a run Cycle Cycle Cycle Cycle The number of finished cycles is shown Lid Lid Lid Lid Temper...
Page 14: ... program name _ date _ time glp if not deleted by the user For this the adjoining window is shown The GLP report can be opened and printed in the GLPs dialogue see chapter 11 7 7 7 Not create GLP Report There will be no GLP report created for the aborted program At the next run the GLP report query will appear again You will return to the Run dialogue which shows the last chosen program which can ...
Page 15: ...orresponding editor fields By entering values 40 C the lid heating will be turned off 8 2 1 Stop Use this button to quit the incubation mode Heating of the thermal plate and lid are then deactivated 8 2 2 Lid lock unlock Use this button to lock or unlock the lid For systems with HPL lids High Pressure Lid there is the option to define the lid pressure in an opening dialogue 8 2 3 Timer The clock s...
Page 16: ...d The operation of the dialogue is as follows The operation of the dialogue is as follows The operation of the dialogue is as follows The operation of the dialogue is as follows 9 1 1 Open a program Saved programs can be selected with this button By touching the desired program it will be selected of the file list and opened by using the Open button After this the thermal cycler returns to the Pog...
Page 17: ...ined Optionally author user any comment and different program options can be entered These data serve for information and can also be retrieved in the Run dialogue 9 1 4 New folder Use this button to create a new subfolder after selecting an existing folder in which the new subfolder should be created 9 1 5 Delete Programs or folders can be deleted with this button 9 1 6 Info button Further detail...
Page 18: ...the Programs dialogue 9 2 1 Entering a command To enter a new command into a program the desired line has to be selected From the left side of the program window a blue arrow will point on the desired line After selecting the program command of the list in the left window it can be inserted into the desired place via the button Alternatively this can be made by double click on the program command ...
Page 19: ...ration If Power Fail Denaturation is activated by pushing the checkbox the adjoining dialogue appears If a power failure had happened while a program was running the thermal cycler restarts the program at the point it was stopped as soon as the power returns Autorestart after power failure Additionally if the option Power Fail Denaturation is activated the thermal block will heat to the stated tem...
Page 20: ...possibility to adjust temperature regulation for a temperature step accordingly to sample volume If necessary the temperature regulation will perform short term deliberate overshoots and undershoots depending on the entered sample volume during PCR run 9 2 4 7 Emulation Using the Checkbox the thermal cycler will perform PCR like the chosen cycler emulating its performance This function could be us...
Page 21: ... have used previously Tube Control Did you had to enter a volume setting in your cycler XY Tube Control mode If yes please enter the same volume in the peqSTAR in the Program Options under Tube Control Ramp Rate Have you worked on cycler XY with a reduced ramp rate Throughout the whole protocol Then you could adjust the ramp rate in the Program Options under Global Program Ramp for the whole proto...
Page 22: ... in the dialogue window Further information regarding program options can be found at chapter 9 2 4 2 9 2 9 Save Run A program can be saved and run immediately using this button In advance there will be a security check which needs to be confirmed with Yes The program is then loaded to the Run tab and started At the peqSTAR 2X the desired block can be chosen by using this dialogue 9 2 10 Editing a...
Page 23: ... lid will automatically set the pressure on the samples to 50 N Heat Lid 9 3 3 Heat Lid Using this command you can activate or deactivate lid heat and the adjoining window appears By activating Lid heat on it is possible to insert the desired lid temperature The temperature needs to be in a range of 40 120 C When Lid heat on is not activated the lid heat is switched off When creating a new program...
Page 24: ...defines whether the time will elongate or reduce 9 3 5 Elongation Time and Melting Temp The elongation time and the melting temperature of the primers can be calculated by using this button They have to be considered as guideline values Depending on the application of the user further optimization might be necessary Typically the optimal annealing temperature is about 3 C lower than the calculated...
Page 25: ... setting for gradients in the peqSTAR 96X where the complete row from well 1 to well 12 has the same temperature Furthermore there is the possibility of a 16 gradient distribution Different temperatures are regulated in the left and right half of the block leading to a gradient within a single row from the left to the right The temperatures displayed in the software window are only reached in rows...
Page 26: ... left using the button Close Cycle Start Cycle 9 3 8 Start Cycle Manual cycle beginning Close Cycle 9 3 9 Close Cycle Manual cycle ending Pause 9 3 10 Pause Use this button to interrupt the running program temporarily The adjoining window appears where you can enter a temperature value for the pause Store 9 3 11 Store This command keeps the thermal plate temperature on the set value Automatically ...
Page 27: ...ed 10 1 Operation of the Diagnostics dialogue Different operating elements for indication and navigation within a diagram are available Navigation button To the end of the record Navigation button One page forward Navigation button To the beginning of the record Zoom 0 5 fold Zoom 2 fold Time scale Help Mode Lid Lid temperature Cycler Temperature of cycler interior Selection of single view or comp...
Page 28: ...be shown in the diagram window anymore but the record of the corresponding temperatures will be continued 10 2 2 Single view By pushing the button the single view of the zones will be shown Using the single view of the zones the temperature profile of each zone will be shown in separate diagrams The arrangement of the single diagrams match to the arrangement of the zones on the thermal plate 10 2 ...
Page 29: ...11 2 1 Select GLP Select a GLP report shown in the adjoining text window GLP reports can also be copied or deleted and a new folder can be created for GLP reports 11 2 2 Open GLP report Use this button to open a stored GLP report Select the desired GLP report of the file list and open it by pushing the button Open The GLP report is shown as displayed in chapter 11 1 11 2 3 Printer Use this button ...
Page 30: ...be copied Several GLP reports files can be marked via mouse click simultaneously if the option Multiple Selection was chosen Marked GLP reports files are shown with a blue background By using the Copy button the adjoining dialogue appears Pushing OK the files will be copied the operation can be aborted with Cancel 11 2 6 New folder A new folder can be created using this button ...
Page 31: ...wing dialogue 12 1 Time Date Setting of actual time and date By pushing the clock button the adjoining window appears and time and date can be set 12 2 Show Hide mouse cursor Use the button to show or hide the cursor on the screen 12 3 User Call Using this button different user calls can be set 12 3 1 Sound Selecting the tab Sound the adjoining window opens and the settings for sounds can be chang...
Page 32: ...rence of the corresponding event Note The peqSTAR X needs to be installed in a network with access to the internet so that it can send an email to the user when needed Please check settings under System Network as for example the entry of the DNS Server is necessary for sending emails Please ask the administrator for the required settings Please ask the administrator for the required settings Plea...
Page 33: ...ton a test mail is sent to check whether the settings are correct and emails could be send by the cycler In case the test mail could not be sent successfully please ask your network administrator for the required settings 12 4 User Management In the user accounts management user groups or single users can be configured and their settings e g right of access reading programs edit programs etc will ...
Page 34: ...e checkbox Auto logout after the user can define a time span when the logged in user is automatically logged out Originally the password for the Originally the password for the Originally the password for the Originally the password for the administrator is admin administrator is admin administrator is admin administrator is admin 12 4 1 Change User Using this dialogue window users can be changed ...
Page 35: ...seful to set up a new user group with the appropriate rights to access see chapter 12 4 3 12 4 2 3 Administration of user groups See chapter 12 4 3 12 4 2 4 User Information Use this button to get further information on the user user name full name description and group membership 12 4 2 5 Delete a user Select a user and remove it with this button For safety reasons a query appears if the user sho...
Page 36: ...onfigurations 12 4 3 2 Delete a group Select the group and delete it using this button For safety reasons a query appears if the group should be removed for sure 12 4 3 3 Show and change group information 12 4 4 User logout If a user logs out by using the button Logout the user Guest is automatically logged on The user Guest initially does not have any rights This is to make sure that a new user w...
Page 37: ...or access to a released network device can be entered Username to log on the network Password to log on the network Network path which is available for the user as additional path for cycler pro grams and GLP reports after the successful network connection This path is assembled of IP address where release network path is located directorypath Please note that there need to be the corresponding ac...
Page 38: ...eqSTAR 2X Press OK after the test has finished If one or both results are not OK the overtemperature protection is damaged In In In In this case the therm this case the therm this case the therm this case the thermal al al al cycler must not cycler must not cycler must not cycler must not be be be be used anymore and must be sent back to the used anymore and must be sent back to the used anymore a...
Page 39: ...rtion dialogue of a new temperature step This value can be changed manually in the PCR program 12 7 1 2 2 GLP Settings Setting a check mark in this box a GLP report will be created after every run after the query If the box is not marked no GLP report will be created No according query will appear 12 7 1 2 3 Factory Setting The configurations set under Firmware Control Unit Settings can be changed...
Page 40: ...onnected to the device Next it will be asked whether the software of power unit should be updated If you confirm with yes the update starts Important Important Important Important The USB memory stick may not be removed during the update process The USB memory stick may not be removed during the update process The USB memory stick may not be removed during the update process The USB memory stick m...
Page 41: ...log txt can be saved on a USB stick Alternatively possible error messages can be shown by pressing the button Show Error Messages 12 11 LAN Control This dialogue is used to connect with a thermal cycler via network or zigbee to be able to remote control the instrument When instruments should be available using the instrument button at the top of the display the checkbox under Show needs to be acti...
Page 42: ...trument buttons at the top of the display the checkbox under Show needs to be activated As soon as the cycler is displayed it can be remote controlled by the control unit 12 11 2Lan Connect As soon as a cycler was once remote controlled via LAN connection it will be displayed in the LAN control window in our example peqSTAR 2X 192 168 0 121 and marked as non connected For a new LAN connection with...
Page 43: ...owing message appears The connection to IP Address Remote Cycler will be done as soon as the other part confirms the request Then the following message appears on the system that shall be controlled Do you want to accept connection to IP Address Control Unit COM Interface COM Interface COM Interface COM Interface As COM number use the COM port where the ZigBee dongle was installed this can be chec...
Page 44: ...s the cycler is displayed it can be controlled via operating software as usual 12 11 6 Zigbee Disconnect To disconnect a ZigBee connection with a cycler mark the chosen unit in the overview and use the button Zigbee Disconnect The cylcer could then be controlled again via touch screen Alternatively the LAN connection will be stopped when the cycler will be switched off or in case that the software...
Page 45: ... may only be carried out by authorized persons using original replacement parts The replacement of single components of the device must not be done by the user but exclusively by authorized specialists Therefore the device must be sent to the manufacturer The surface of the device should be The surface of the device should be The surface of the device should be The surface of the device should be ...
Page 46: ...anism 4 x USB 1 x Ethernet interfaces MS Windows or Linux maximal length of USB cords 3 m Note USB interfaces only support standard USB sticks cord mouse and keyboards Dimensions B x H x D 30 x 28 x 38 cm Weight incl block 13 3 kg Electrical power supply 90 264 V AC 50 60 Hz 850 VA Fuse 10 A time lag Pollution rate 2 Environmental temperature range 10 C to 30 C Max relative humidity 70 Maximum hei...
Page 47: ...atic height adaption for 48 x 0 2 ml tubes 48 well PCR plates or 24 x 0 5 ml tubes with flat caps peqSTAR 96X 96X Gradient 96 well Universal block with lid heating and automatic height adaption for 96 x 0 2 ml tubes 96 well PCR plates or 48 x 0 5 ml tubes with flat caps peqSTAR 96X HPL 96X HPL Gradient 96 well block with High Pressure lid HPL 100 250 N for the safe sealing of 96 well PCR plates pe...
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