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PS-3224

 Calibration: See www.pasco.com/calibration

11

013-16057A

For a one-point calibration, you will need one “known standard” with 100% dissolved oxygen saturation. 

For a two-point calibration, you will need two “known standards”; one with 100% dissolved oxygen saturation, and 
one with 0% dissolved oxygen saturation.

Known Standards (100% dissolved oxygen saturation and 0% dissolved oxygen saturation)

One method for a known standard with 100% dissolved oxygen saturation is to use water-saturated air. Moisten the 
sponge inside the end of the Probe Cover (Rubber Boot) with water, and then put the probe into the Probe Cover 
for about fifteen minutes. WARNING: Don’t calibrate the sensor if the end of the Sensor Cap is under pressure. 
Therefore, do not push the Probe into the Rubber Boot so far that it compresses the water-moistened sponge.

A second method for a known standard with 100% dissolved oxygen saturation is to use air-saturated water. 
Connect an air-stone to an air pump, and put the air-stone into water. Allow the air-stone and air pump to run for 10 
minutes to make sure that the water is completely saturated. 

To make a known standard with 0% dissolved oxygen saturation, dissolve enough sodium sulfite, sodium bisulfite, 
or potassium sulfite in 150 milliliters of distilled water such that a small amount of sodium sulfite remains 
undissolved in the bottom of the beaker. WARNING: Use the same standard lab safety procedures for handling 
sulfite compounds as for handling any chemical compound.

PASCO Capstone Calibration. Remember, see www/pasco.com/calibration

Capstone Calibration Steps

Place the Wireless Optical Dissolved Oxygen Sensor into your known standard for 100% dissolved oxygen 
saturation (for example, moisten the sponge inside the end of the Probe Cover (Rubber Boot) with water, and 
then put the probe into the Probe Cover for about fifteen minutes,)

In PASCO Capstone, click the Calibration icon in the Tools palette to open the Calibration panel. 

1.

The first step in the Calibration panel says 
“Choose the type of measurement you would like 
to calibrate:” The default for the sensor is “Satura-
tion”. Click “Next”. 

2.

The second step says “Choose the probes you 
would like to calibrate now:”.The, Wireless Optical 
Dissolved Oxygen Sensor is automatically 
selected (if the default measurement is kept). 
Move to the third step.

3.

The third step says “Choose the type of calibration 
you would like to perform:” Select “

One Standard 

(1 point slope)

”. Click “Next”. 

4.

NOTE: The first point of calibration is already set, 
so the fourth step says (Calibrate the second 
point:) 

Summary of Contents for PS-3224

Page 1: ...ommended Equipment Optical Dissolved Oxygen Metal Guard PS 3605 Required Item Part Number PASCO Data Collection Software see www pasco com Battery LED Bluetooth LED Micro USB Cable Transparent Cover E...

Page 2: ...ensor including the following Barometric pressure is recorded and stored only when the sensor is initially connected via Bluetooth or USB Initial Step Charge the Battery Connect the Cable Remove the t...

Page 3: ...In Sparkvue open the Main Menu and select Help for information about collecting displaying and analyzing data In the window that opens is the address of the web page for Help Copy and paste the addre...

Page 4: ...vue and PASCO Capstone support logging Check the PASCO Web page at www pasco com software for the latest software version Set Up the Software SPARKvue Set Up Connecting the Sensor to a Tablet or a Com...

Page 5: ...sor to a Computer with the Micro USB Cable Connect the micro end of the included Micro USB Cable into the micro USB port on the top of the sensor Connect the other end of the Micro USB Cable to a USB...

Page 6: ...issolved Oxygen Sensor Set Up the Software 6 013 16057A Select a Layout and then select a data display In the Data Display Click Select Measurement Select a measurement from the list under Sensors Sel...

Page 7: ...communicating with the computer software or tablet application try restarting the software or application If the problem remains press and hold the ON button on the sensor for 10 seconds and then rel...

Page 8: ...c pressure and salinity Temperature The rate of diffusion of oxygen through the Sensor Cap changes with temperature approximately 1 5 per degree celsius The Optical Dissolved Oxygen Probe has a built...

Page 9: ...int free cloth If necessary use a mild detergent Do not use alcohols or other organic solvents that may deteriorate the oxygen permeable diffusion layer and the sensing layer dye layer When changing t...

Page 10: ...ure of the gas in wet air or in solution is taken to be 20 9 Given this it is reasonable to use the probe in a gaseous environment to measure the oxygen present The sensor outputs a value labeled O2 G...

Page 11: ...ssolve enough sodium sulfite sodium bisulfite or potassium sulfite in 150 milliliters of distilled water such that a small amount of sodium sulfite remains undissolved in the bottom of the beaker WARN...

Page 12: ...connect pair and calibrate the other sensor s if necessary During the calibration process if you click Calibrate first point or Calibrate second point and then click Cancel the sensor will be restored...

Page 13: ...RKvue Calibration Steps Place the Wireless Optical Dissolved Oxygen Sensor into your known standard for 100 dissolved oxygen saturation 1 In SPARKvue touch or click Sensor Data in the Welcome Screen a...

Page 14: ...rdware Setup icon to open the Configure Sensors and Measurements window 5 In the Configure sensors and measurements window D02 Saturation is the default choice for calibration Touch or Click the Calib...

Page 15: ...the Calibrate Sensor Enter Values window Calibration Point 2 is the default selection with the Standard Value 100 0000 and the Sensor Value panel NOTE that the Sensor Value panel will show the number...

Page 16: ...ight arrow in the Configure sensors and measurements panel to close the panel and return to the data dis play 2 In the data display click the up arrow menu icon in the Live Data Bar to open the menu t...

Page 17: ...ing caused by exposure to light so the Sensor Cap must be replaced periodically It should also be replaced if it is cracked scratched or damaged The Sensor Cap is warranted for one year and may last m...

Page 18: ...Cap Calibration After a new Replacement Sensor Cap PS 3604 is placed on the Optical Dissolved Oxygen probe there are two parts to the Sensor Cap Calibration process First enter the Sensor Calibration...

Page 19: ...ace between each number NOTE Enter the numbers exactly as they appear on the sheet with the Calibration Code Coefficients for Cap Replacement including negative signs decimal points and other characte...

Page 20: ...n numbers from the sheet with the Calibration Code Coefficients for Cap Replacement as a list of 10 numbers separated by spaces NOTE Enter the numbers with a space between each number NOTE Enter the n...

Page 21: ...r calibration above 200 10 Resolution 0 01 mg l or 0 1 saturation Temperature C Range 0 50 Accuracy 0 1 C Resolution 0 1 C Barometric Pressure inHg Optional Units mmHg Range 375 836 mmHg Accuracy TBD...

Page 22: ...not be disposed of in a standard waste container Battery Disposal Instructions Batteries contain chemicals that if released may affect the environment and human health Batteries should be collected se...

Page 23: ...ng lower than 0x6 means an older version of Bluetooth Your device will need the PS 3500 USB Bluetooth 4 0 Adapter 1The Mac Mini and MacBook Air were updated with Bluetooth SMART support in 2011 The Ma...

Page 24: ...Wireless Optical Dissolved Oxygen Sensor Appendix A 24 013 16057A...

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