Panasonic MLS-3751, Operating Instructions Manual, Page: 13 / 32

Capacity of culture mediums and containers
Pour less liquid in the container than the specified volume.
Set the timer to a longer time than usual.
There is a time lag (time difference) from when the temperature in
the chamber reaches the sterilization temperature until the
temperature of the whole culture medium reaches the sterilization
temperature.
Excessive liquid will spill when heated.
Sterilization of large volume culture mediums
Sterilization with the use of plastic containers
Prolong the sterilization setting time even more. Otherwise, poor
heat conduction of the container may cause incomplete
sterilization.
Melting of solidified general agar mediums
Refer to the below table to set the melting time (per flask).
Test of Durham tube
Air remaining in the Durham tube can be purged by setting the
exhaust temperature and exhaust rate to appropriate levels.
Agar media in 500 ml flask x 1~21, or
Agar media in 1000 ml flask x 1~12
11 min. 8 min.
Liquid Volume Time Lag
Melting Temperature Capacity
2 L
100 ° C
1 L
60 min.
45 min.
Melting Time
An
“
Object temperature sensor
”
that detects the temperature
of objects to be sterilized and controls sterilization time is available
as an option. Even after this sensor has been installed, control can
be switched over to the product
’
s own sensor by pressing the
object temperature ON/OFF button. Use this optional sensor to
ensure sterilization is more reliable. For details, contact your
dealer.
Recommendation
Sterilization and melting of liquids such
as culture mediums, etc.
MLS-3751 MLS-3781
Sterilization Setting Time = Sterilization Time + Time Lag
Porous caps
Culture medium
temperature
Time
121 ° C
100 ° C
Temperature
Sterilization
setting time
Time lag
Sterilization
time
Plastic
container
Durham tube
Temperature inside
the chamber
Sterilization Objects
13
WARNING
・
Do not seal the object container tightly.
Use a porous cap or loosen the cover sufficiently.
Failure to observe this warning may result in accident or incomplete sterilization.
Failure to observe this warning may result in accident or
incomplete sterilization.
・
Do not sterilize objects in a steam impermeable
container or bag.
Failure to observe this warning may destabilize control of the
product or result in incomplete sterilization.
・
Do not block the holes or temperature sensor in the
chamber with objects.
・
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