MiPNet22.11
O2k-FluoRespirometer manual
2
Oroboros Instruments
Mitochondria and Cell Research
HRFR with the Oroboros O2k-FluoRespirometer is based on a radically
different systems approach to polarographic measurement of oxygen
dynamics. HRFR rests on tightly connected instrumental features and
concepts integrated by the DatLab 7 software, providing real-time display
of oxygen concentration and respiration (oxygen flux), and a fluorometric
signal and its time derivative (slope). The mechanics, critical selection of
materials, and state-of-the-art electronics provide the basis of signal
stability of an optimized polarographic oxygen sensor (
OroboPOS
) and
Smart Fluo-Sensors
in an electronically controlled thermal environment
with high temperature stability. Calibration and quality control follow
standardized operating procedures (
O2k-SOP
) with automatic real-time
analysis supported by DatLab. Scientific support complements HRFR.
Detailed laboratory
O2k-Protocols
provide step-by-step instructions,
integrated as DL-Protocols into DatLab 7. Numerous new
O2k-
Publications
on applications of the Oroboros O2k focus on critical issues
of mitochondrial physiology.
While the Oroboros O2k-FluoRespirometer provides the instrumental
basis for High-Resolution FluoRespirometry, successful operation at high
accuracy up to the limit of detection depends on a professional application
by the technician, scientist or student. Participation in a hands-on
introductory
O2k-Workshop
is recommended to save time and gain from
the experience obtained over many years of application in various settings.
The Oroboros O2k is the modular
2-chamber system for High-
Resolution
FluoRespirometry
(HRFR). O2k-FluoRespirometer
:
simultaneous monitoring of oxygen
consumption and fluorescence with
small amounts of samples (isolated
mitochondria to permeabilized tissues
and cells).
Included
: O2k-Titration
Set, Titration-Injection microPump
(
TIP2k
), Integrated Suction System
(ISS).
Add-on O2k-Modules:
high
flexibility to expand the spectrum in
HRFR.
O2k-Manual user information
PLEASE STUDY THIS MANUAL
»
wiki.oroboros.at/index.php/MiPNet07.08_User_information
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wiki.oroboros.at/index.php/O2k-Manual
Summary of Contents for O2k-FluoRespirometer
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