Oroboros Instruments O2k-FluoRespirometer, Manual

Page: 11 / 20

MiPNet22.11 O2k-FluoRespirometer manual 11
Oroboros Instruments High-Resolution FluoRespirometry

Amplification options are 1, 2, 4, or 8 V/µA, for signal
amplification in specific cases (previous O2k-Series).
Polarization voltage [mV] The default for the POS is 800 mV, which
is maintained in all routine experiments. Any changes
affect the calibration. The voltage can be modified in the
range of
 2,000 to +2,000 mV for scanning the plateau
region of the polarographic oxygen sensor, or for
different types of sensors.
Oxygen sensor # The number of the polarographic oxygen sensor is
shown here for chambers A and B. To change them, L

click on Configuration and enter the sensor number.
6.2.3. O2k control \ Tabs: Amperometric and Potentiometric
Amp and pX Settings for the Amp and pX tabs are described in
• MiPNet17.05 O2k-Fluo LED2-Module
• MiPNet15.05 O2k-NO Amp-Module
• MiPNet15.03 O2k-MultiSensor-ISE
6.3. Connect and save a DLD file
In an automatically opened Save as window, the default
file name has the form:
2017-07-21 P1-01.DLD
Year-Month-Day P#-##.DLD: P#= ower-O2k; ##
=sequential number of the experiment; extension DLD
for D at L ab D ata.
Select a subdirectory, optionally edit the file name and L

Save. This initiates data recording.
COMM LED of the O2k-Main Unit
● Yellow USB connection is available, but DatLab connection
(measurement) is not started.
● Green USB connection to a PC is established and a Datlab
measurement is in progress.
● Red flashing ( 1 s on/ 1 s off) : USB connection was lost during
a Datlab measurement (USB communication error or USB
unplugged). If Datlab reconnects automatically, the
COMM LED remains green without flashing.
6.4. Experiment
After connecting to the O2k the Edit experiment window
pops up. Edit experimental code, sample information,
medium, and additional information. Entries can be edited
at any time during real-time or post-experiment analysis.
All related results are recalculated instantaneously with
the new parameters.
0:30 [h:min]
1:15 1:10 1:05 1:00 0:55 0:50 0:45
4B
:
O2
co
n
ce
n
tr
ati
o
n
[µ
M
]
200
160
120
80
40
0
4B
:
O2
fl
u
x
p
er
V
[p
mo
l/
(s*
ml
)]
100
80
60
40
20
0
6G 7S 8Gp
c P G S Gp U1 U1.5 U2 Rot