Oroboros Instruments O2k-FluoRespirometer Manual Download Page 13

MiPNet22.11 

O2k-FluoRespirometer manual

 

13

 

 

 

Oroboros Instruments

 

 

High-Resolution FluoRespirometry

 

 

O2k  configuration  window.  The  background  is  green  if 

rotation of the stirrer is on, briefly yellow when the stirrer 
is accelerating, red if the stirrer is switched off.

 

 

O2 calib.

 

Yellow indicates that the POS has not been calibrated in 
the present record. Calibration values are used as default 

from files previously saved real-time.

 

L



 

Left  double-click  on  O2  calib.  to  open  the  calibration 
window (left or right).

 

 

O2 calib.

 

Green indicates that the POS has been calibrated within 

the file. It can be re-calibrated any time when connected 
or disconnected to the O2k.

 

 

Set temp. 

 37.0 indicates the temperature setting sent from the O2k 

control  window,  green  if  the  measured  temperature  is 
within ±0.002 °C of the set temperature, but yellow if the 

difference is more than ±0.002 °C.

 

 

Illum. on / Illum. off indicates whether the chamber illumination (not to 

be  confused  with  light  sources  for  fluorescence)  is  on 
(Illum.on,  highlighted in  yellow) or off (Illum.off, grey). 
Toggle by a L



 double-click. When the windows to the 

glass  chambers  are  closed  with  optical  sensors,  the 
illumination is not visible and must be switched off.

 

 

O2k: Pan auto indicates that automatic panning is on (green) with the 

O2k connected to DatLab. The time range is maintained 
while the time axis always shows the currently recorded 

data. The value of the offset (minimum time) increases 
stepwise as experimental time proceeds.

 

 

O2k: Pan off: Yellow indicates that automatic panning is off. Plots are 

shown in static display. This allows for manually panning 
backwards to observe previous sections of the experiment 

at  a  given  time  range.  In  this  mode,  the  actual 
experimental time may be off-scale. Toggle between Pan 

auto

 

and

 

Pan off by a L

 click on the text. This does not 

influence continuous data recording.

 

 

TIP: Ready

 

Indicates that a Titration-Injection microPump (TIP2k) is 
connected to the O2k, and is ready to start F8. For TIP2k 
instructions see 

MiPNet12.10

.

 

 

ERROR

 

Blinks red if an error has occurred. In this case, L

 click 

on  ERROR  to  open  the  Error  message  window.  In  this 
window, L

 Close to remove the blinking error signal.

 

 
 

 

Summary of Contents for O2k-FluoRespirometer

Page 1: ...meter provides a unique high resolution approach to the monitoring of cellular and mitochondrial respiratory function High Resolution FluoRespirometry HRFR is indispensable for application of SUIT pro...

Page 2: ...y step instructions integrated as DL Protocols into DatLab 7 Numerous new O2k Publications on applications of the Oroboros O2k focus on critical issues of mitochondrial physiology While the Oroboros O...

Page 3: ...Titration Set 1 Unpack the O2k FluoRespirometer Box 1 or Peli Case contains the O2k Main Unit with the O2k Chambers in a finally mounted position the OroboPOS Connectors without polarographic oxygen...

Page 4: ...ates this icon on your desktop Oroboros DatLab for control of the O2k TIP2k data acquisition analysis Running DL7 For data safety reasons and optimal DatLab performance it is strongly recommend to ope...

Page 5: ...e for the ISS O2k Titration Set and handling For maximum signal stability and optimal temperature control the O2k should be placed in a position in which it is not exposed to direct sunlight and sudde...

Page 6: ...ct to electrical ground or simply touch the O2k steel housing 5 1 Instrument overview The O2k FluoRespirometer is electronically equipped with all required plugs for sensors in addition to the O2 chan...

Page 7: ...it Insert the male plug of the cable 2 into the female O2 plug The red dot on the male plug 2 faces straight upwards Each OroboPOS head can be used on O2k chamber A or B Note the POS number marked on...

Page 8: ...cable to the Fluo plug Similar to the OroboPOS 2 insert the male plug of the cable into the female Fluo plug The red dot on the male plug faces straight upwards Each Smart Fluo Sensor can be used on...

Page 9: ...or chambers A and B Usually sensors should not be switched between chambers except for troubleshooting The individual sensor number is included in the protocol for generating a database of calibration...

Page 10: ...w rpm but uncoupling of the magnet occurs at very high stirring speeds causing high signal noise Both chambers may be set at an identical stirrer speed Data recording interval s can be set between 0 2...

Page 11: ...n automatically opened Save as window the default file name has the form 2017 07 21 P1 01 DLD Year Month Day P DLD P ower O2k sequential number of the experiment extension DLD for DatLab Data Select a...

Page 12: ...metric pressure continuously recorded Peltier power Operative Peltier power applied for temperature regulation of the O2k block continuously recorded Amp A and Amp B V Amperometric signal from amperom...

Page 13: ...is on Illum on highlighted in yellow or off Illum off grey Toggle by a L double click When the windows to the glass chambers are closed with optical sensors the illumination is not visible and must b...

Page 14: ...ps for renaming or deleting O2k setups 7 2 O2k control F7 with the O2k connected The O2k control key F7 provides fast access to the O2k control window Load setup After selecting a setup it must be L l...

Page 15: ...ibed above with the next sequential number After re connecting the O2k continues operation uninterrupted the experimental time is reset to zero and data recording is restarted with automatic saving to...

Page 16: ......

Page 17: ...t O2k Chamber Stirrer Bar white PVDF 15x6 mm OroboPOS Holder O2k Chamber Holder A2_Insert OroborPOS OroboPOS Connector A3_O2k Chamber volume calibration Volume Calibration Ring Stopper black PEEK coni...

Page 18: ...chamber into the copper block of the O2k FluoRespirometer 2 Screw the POS holder 1 into a position where the glass chamber with the angular cut towards the POS holder is slightly lifted upwards 3 Scre...

Page 19: ...of the stopper black PEEK is not part of the effective chamber volume When titrating 2 1 ml insert the tip of the pipette to the wall of the glass chamber to add the entire volume Liquid must not be l...

Page 20: ...the manual Trouble shooting of test versions DatLab 7 1Andras M sz ros Oroboros Instruments AM wrote and edited the manual Taking editing photos and images 1Ondrej Capek Oroboros Instruments OC was m...

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