Oroboros Instruments
High-Resolution FluoRespirometry
O2k-FluoRespirometer manual
Mitochondrial Physiology Network 22.11(01):1-16 (2017)
Version 01: 2017-07-21 O2k-Series H DatLab 7
2017 Oroboros
wiki.oroboros.at/index.php/MiPNet22.11_O2k-FluoRespirometer_manual
O2k-FluoRespirometer
manual
Gnaiger E
1,2
, Sigl R
1
, Mészáros A
1
,
Capek O
1
, Gradl L
3
, Gradl P
4
Oroboros Instruments
Schöpfstr 18, A-6020 Innsbruck, Austria
Email:
www.oroboros.at
Mitochondria and Cell Research
Section
1
.
Unpack the O2k .......................................... 3
Page
2
. Oroboros USB-flash drive and DatLab ............ 3
3.
Electric and lab space requirements ............... 4
4.
Start the O2k .............................................. 6
5.
Assembly of the O2k .................................... 6
6.
Start DatLab ............................................... 9
7.
O2k control ............................................... 14
Supplement A: Videosupport ............................. S1
Supplement B: O2k-Chamber assembly .............. S2
Supplement C: O2k-Chamber volume calibration . S3
Overview
The
Oroboros O2k-FluoRespirometer
provides a unique high-resolution
approach to the monitoring of cellular and mitochondrial respiratory function.
High-Resolution FluoRespirometry
(HRFR) is indispensable for application of
SUIT protocols, at low respiratory activities, kinetic transitions, and controlled
oxygen levels. High-resolution is required for analysis of pathological effects
causing reduced respiration (mitochondrial and metabolic diseases,
ischemia-reperfusion injury, oxidative stress, apoptosis, aging). HRFR is
unique for simultaneous measurement of respiration and fluorometric
signals
(H
2
O
2
flux,
mt-membrane
potential,
Ca
2+
,
ADP-ATP
phosphorylation), for analysis of biopsies with limited amounts of sample
(diagnosis of genetic and acquired mitochondrial defects in pediatric
patients, diabetes); cell cultures with limited cell numbers or low activities;
mutants with diminished respiratory capacity; chemical oxidation rates and
antioxidant capacities and oxygen kinetic measurements at low,
physiological intracellular oxygen levels.
O2k-FluoRespirometer
Summary of Contents for O2k-FluoRespirometer
Page 16: ......
