6
5. Pipet up and down 5-10 times or vortex for 30 seconds.
6. Let sit at room temperature for 5 minutes.
7. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Total Pure NGS. Let sit at room temperature until the Mag-Bind® Total Pure NGS is
completely cleared from solution.
8. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Total
Pure NGS.
9. With the plate remaining on the magnet, add 200 μL 70% ethanol to each well.
10. Let sit at room temperature for 1 minute. It is not necessary to resuspend the Mag-
Bind® Total Pure NGS.
11. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Total
Pure NGS.
12. Repeat Steps 9-11 for a second 70% ethanol wash step.
13. Leave the plate on the magnetic separation device for 5-15 minutes to air dry the
Mag-Bind® Total Pure NGS. Remove any residual liquid with a pipettor.
Note:
It is important to dry the Mag-Bind® Total Pure NGS before elution. Residual
ethanol may interfere with downstream applications.
Optional:
Incubating the plate at 37°C can speed up evaporation.
14. Remove the plate from magnetic separation device.
15. Add 30-40 µL Elution Buffer (not provided) to each well.
16. Pipet up and down 20 times or vortex for 30 seconds.
Mag-Bind® Total Pure NGS - 96-well Plate Protocol
