11
Troubleshooting Guide
Please use this guide to troubleshoot any problems that may arise. For further assistance,
please contact the technical support staff, toll free,
at
1-800-832-8896
.
Possible Problems and Suggestions
Problem
Cause
Solution
Low yield
Low PCR product yield
Increase the number amplification cycles
for PCR
Smaller PCR product size Small PCR fragments normally give lower
yield.
Ethanol residue
During the drying step, remove any
liquid from bottom of the well.
Particle loss during the
procedure
Increase magnetization time.
Aspirate slowly.
DNA remains bound to
beads
Increase elution volume.
Incomplete resuspension
of the beads during
elution
Vortex or pipet up and down to fully
resuspend the beads.
Problem
Cause
Solution
Primer
carryover
Insufficient wash of the
particles
Wash the beads one more time with 70%
ethanol.
Problem
Solution
Non-specific
amplification
products were
not removed
The size of the non-
specific amplification
products are larger than
100 bp
Non-specific amplification products
larger than 100 bp are not efficiently
removed from PCR products.
Problem
Cause
Solution
Problems in
downstream
applications
Salt carryover
70% ethanol must be stored at room
temperature.
Ethanol carryover
Ensure the beads are completely dried
before elution.
