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Mag-Bind® Total Pure NGS - 384-well Plate Protocol
Mag-Bind
®
Total Pure NGS Protocol for 384-well Plates
Materials and Equipment to be Supplied by User:
•
384-well PCR plate containing PCR samples (up to 100 μL/well)
•
Magnetic separation device for 384-well PCR plates
•
Skirted 384-well PCR plate
•
Vortexer
•
Multichannel pipettor
•
Multichannel disposable reservoirs
•
Sealing film
•
70% ethanol
•
Elution Buffer (Cat#PDR048 or 10 mM Tris pH 8.0),TE Buffer, or nuclease-free water
Before Starting:
•
Bring the Mag-Bind® Total Pure NGS to room temperature before use.
1. Read the manufacturer’s instruction manual for the magnetic separation device, if
provided.
2. Place the 384-well PCR plate on the bench and measure the volume of the PCR
reaction. Transfer the sample to a skirted 384-well PCR plate.
3. Shake the Mag-Bind® Total Pure NGS to resuspend any Mag-Bind® Total Pure NGS
particles that may have settled. Allow Mag-Bind® Total Pure NGS to come to room
temperature before use.
4. Add the desired volume of Mag-Bind® Total Pure NGS to each well based upon
size of fragments to recover. Adding more Mag-Bind Total Pure NGS allows smaller
fragments to bind to magnetic beads while the larger fragments will continue to
bind. Volume to add to the sample is normaly determined by the next generation
sequencing library construction instruction manual.
Example:
1.2X ratio required: 50 µL sample x 1.2 = add 60 µL Mag-Bind® Total Pure
NGS
