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5
Mag-Bind® Total Pure NGS - 96-well Plate Protocol
Mag-Bind
®
Total Pure NGS Protocol for 96-well Plates
Materials and Equipment to be Supplied by User:
•
96-well PCR plate containing PCR samples (up to 50 μL/well)
•
Magnetic separation device (Recommend AlpAqua Cat# 001322). For elution volumes
<30 µL AlpAqua Magnum FLX (Cat#A000400) is recommended.
•
96-well microplate or PCR Plate for elution
•
Vortexer
•
Multichannel pipettor
•
Multichannel disposable reservoirs
•
70% ethanol
•
Elution Buffer (Cat#PDR048 or 10 mM Tris pH 8.0),TE Buffer, or nuclease-free water
Before Starting:
•
Bring the Mag-Bind® Total Pure NGS to room temperature before use.
1. Read the manufacturer’s instruction manual for the magnetic separation device, if
provided.
2. Place the 96-well PCR plate on the bench and measure the volume of the PCR
reaction. Determine the volume of Mag-Bind® Total Pure NGS that will be added to
the reaction. If the reaction volume will exceed 200 µL transfer to a microtiter plate
for processing.
Note:
PCR reactions >20 µL will need to be transferred to a processing plate.
3. Shake or vortex the Mag-Bind® Total Pure NGS to resuspend any particles that may
have settled. Allow Mag-Bind® Total Pure NGS to come to room temperature before
use.
4. Add the desired volume of Mag-Bind® Total Pure NGS to each well based upon
desired fragment size to recover. Adding more Mag-Bind® Total Pure NGS binds
smaller fragments while using less will exclude smaller sizes. Volumes to add to
the sample is determined by the next generation sequencing library construction
instruction manual.
Example:
1.2X ratio required: 50 µL sample x 1.2 = add 60 µL Mag-Bind® Total Pure
NGS