Olympus BH2 Series, Manual

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Phase Contrast on the Olympus BH-2 Microscopes Revision 1 Page 2 of 6
Table of Contents
Introduction ............................................................................................................................................................................ 3
Scope of this Document ...................................................................................................................................................... 3
What is Phase Contrast? ......................................................................................................................................................... 3
How Does Phase Contrast Work? ........................................................................................................................................... 3
Phase Contrast Types .............................................................................................................................................................. 3
Setting Up for Phase Contrast Microscopy ............................................................................................................................. 3
Match Selector Dial with the Low-Power Objective ........................................................................................................... 4
Replace Eyepiece with Phase-Centering Telescope ............................................................................................................ 4
Focus the Phase-Centering Telescope ................................................................................................................................ 4
Center the Selected Phase Annulus .................................................................................................................................... 5
Center the Remaining Phase Annuli ................................................................................................................................... 5
Replace Centering Telescope with the Eyepiece ................................................................................................................ 5
Place Green/Yellow Filter into Filter Receptacle ................................................................................................................ 5
Using Phase Contrast .............................................................................................................................................................. 6
Periodically Verify Centering of the Phase Annuli .............................................................................................................. 6
About the Phase Contrast Filter .......................................................................................................................................... 6
Phase Contrast Artifacts .......................................................................................................................................................... 6
How To Contact the Author .................................................................................................................................................... 6
Table of Figures
Figure 1 – Set condenser dial to match the objective ............................................................................................................ 4
Figure 2 – Replace right eyepiece with phase telescope ........................................................................................................ 4
Figure 3 – Phase telescope improperly focused ..................................................................................................................... 4
Figure 4 – Adjust knurled focus ring to focus the image ........................................................................................................ 4
Figure 5 – The phase telescope is properly focused ............................................................................................................... 4
Figure 6 – Centering screws of BH2-PC/PCD condenser ......................................................................................................... 5
Figure 7 – The phase ring and phase annulus images ............................................................................................................ 5
Figure 8 – The phase annulus properly centered ................................................................................................................... 5
Figure 9 – Place green/yellow filter into filter receptacle ...................................................................................................... 6