Olympus BH2 Series, Manual

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Phase Contrast on the Olympus BH-2 Microscopes Revision 1 Page 4 of 6
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Olympus IF-550 (interference) or 45G533 (absorptive) filter
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One or more Olympus LB phase contrast objectives
Match Selector Dial with the Low-Power Objective
Select the lowest-powered phase contrast objective
available on your microscope. Rotate the selector dial
on the front of the phase contrast condenser until the
number visible on the front of the dial matches the
selected phase contrast objective (see
Figure 1 ). Make
sure the selected number on the dial clicks in place.
Figure 1 – Set condenser dial to match the objective
Replace Eyepiece with Phase-Centering Telescope
Carefully remove the eyepiece from the right-hand
ocular tube and insert an Olympus phase-centering
telescope
1
in its place (see Figure 2 ).
Figure 2 – Replace right eyepiece with phase telescope
Focus the Phase-Centering Telescope
Look into the phase-centering telescope. With the
lighting intensity set to a comfortable viewing level, you
1
Use the “CT - 5” for 23mm tubes and use the “CT - 30” for 30mm
tubes. Note that other manufacturer’s centering telescope s may be
substituted for the Olympus CT, so long as the barrel diameter of the
phase telescope is compatible with your ocular tubes.
should see a bright ring of light and also a darker ring,
similar to that shown in
Figure 3 . Note that the image
may be so blurry that these features cannot be readily
discerned at this point. Also note that the relative
positioning of the two rings may vary from that shown
in
Figure 3 .
Figure 3 – Phase telescope improperly focused
Rotate the knurled focusing ring on the phase-centering
telescope (see
Figure 4 ) until the image seen through
the phase-centering telescope is sharply focused (see
Figure 5 ).
Figure 4 – Adjust knurled focus ring to focus the image
Figure 5 – The phase telescope is properly focused