BA-01X User Manual
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version 1.9
page 27
overcompensating the electrode capacitance in several positions of the THRESHOLD
potentiometer.
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Now the system is preadjusted for measurements. Find a cell!
o
Approach the desired cell. There are several indications that the electrode is very close to
the cell membrane:
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the electrode resistance increases (the bridge balance appears undercompensated)
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extracellular action potentials (APs) are recorded
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the acoustic monitor signal changes
o
Set the BUZZ DURATION potentiometer to one fourth and apply a BUZZ to the
electrode.
o
If you are lucky the tip of the electrode is now inside the cell.
o
If necessary readjust the BRIDGE BALANCE as shown in Figure 12.
o
You read the membrane potential and can apply current pulses to the cell. After
penetration the voltage responses of the cell to the test pulses should reflect the cell
membrane resistance and time constant.
o
Start the experiment.
Figure 12: Adjustment of the bridge balance after penetrating a cell