BA-01X User Manual
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version 1.9
page 26
8. Sample Experiments
In the following the basics of a simple experiment are described either using a sharp or a
suction electrode.
It is assumed that all connections are built as described in chapter 0. Before starting remove
the cell model.
8.1.
Sample Experiment using a Sharp Electrode
Figure 11: Model
circuit
for
intracellular
recording
using
a
sharp
electrode
C
m
: membrane capacitance, C
stray
: electrode stray capacitance, R
EL
: electrode
resistance, R
m
: membrane resistance
o
Connect the electrode cable / holder to the BNC connector and the Ag-AgCl pellet or the
agar-bridge for grounding the bath with GND at the headstage.
o
Make the basic settings (see chapter 0).
Again: It is of major importance that the BA-01X systems are used only in warmed-up
condition, i.e. 30 minutes after turning power on.
o
Adjust BIAS CURRENT to zero if necessary (see chapter 7.1)
o
Reconnect the STIMULUS INPUT and/or the GATE (TTL) STEP SIZE and put an
electrode into the electrode holder.
o
Immerse the electrode into the bath (not in a cell) as deep as necessary during the
experiment. Test the capability of the electrode to carry current (see chapter 7.4),
compensate the potential offset (see chapter 7.2), measure the electrode resistance (see
#10, chapter 3.2) and compensate the input capacitance (see chapter 7.3 and Figure 9).
o
Enable the OSCILLATION SHUT-OFF unit and set the THRESHOLD so that the
OSCILLATION SHUT-OFF unit activates if the system begins to oscillate. Test this by
