BA-01X User Manual
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version 1.9
page 18
6. Passive Cell Model
The BA-01X can be ordered with a passive cell model as an optional accessory. An active cell
model is also available by request (for ref. see Draguhn et al. (1997)).
The passive cell model is designed for use with single electrode amplifiers (BA series, ELC
series) to check the function of the instrument in the following circumstances:
1.
just after unpacking to see whether the instrument has been damaged during transport or
2.
to train personnel using the instrument or
3.
in case of trouble (see also chapter 9) to check which part of the setup does not work
correctly e.g. to find out whether the amplifier or headstage is damaged or something is
wrong with the electrodes or holders etc.
The passive cell model consists only of passive elements, i.e. resistors that simulate the
resistance of the cell membrane and the electrodes, and capacitances that simulate the
capacitance of the cell membrane. A switch allows simulation of two different cell types: a
cell with 50 M
Ω
and 22 pF (CELL 1, represents an astrocyte like cell) or a “small” cell with
200 M
Ω
membrane resistance and 100 pF membrane capacitance (CELL 1, represents an
neuron like cell), or. Electrode immersed into the bath or SEAL formation can be mimicked
as well. The headstage of the amplifier can be connected to one of two different types of
electrodes (see below).
6.1.
Cell Model Description
Figure 7: passive cell model
1, 3:
connectors for the headstage, 1: electrode resistance: 50 M
Ω
, 3: electrode resistance:
10 M
Ω
2:
GND ground connector, to be connected to GND jack of the headstage
4:
CELL: switch for cell membrane representing a membrane of either 50 M
Ω
and 22 pF
(CELL 1) or 200 M
Ω
and 100 pF (CELL 2).
5:
In GROUND (upper) position the electrodes are connected to ground via a 1 k
Ω
resistor. In SEAL (lower) position are connected to a 1 G
Ω
resistor simulating the
formation of a GIGASEAL with a patch electrode.
