23
II. Microscopy Method
4
For the color contrast microscopy, perform the
following.
1
Place the NCB filter into the optical path.
(See Page 44.)
2
Push in the lambda plate slider to place the lambda
plate into the optical path. (See Page 62.)
This microscopy method makes the background
color of the field of view a sensitive color and
enables the microscopy with a high color contrast.
If the refraction index or the thickness of the
specimen changes, interference color corresponding
to the amount of change is seen.
5
Return to the bright-field microscopy under the
episcopic illumination.
1
Pull out the analyzer slider to remove the analyzer
from the optical path. (See Page 60.)
2
Pull out the polarizer slider to remove the polarizer
from the optical path. (See Page 57.)
3
Pull out the DIC slider to remove the DIC prism
from the optical path. (See Page 65.)
4
Pull out the lambda plate slider to remove the
lambda plate from the optical path.
(See Page 62.)
5
Operate the EPI brightness switch on the
operation panel to adjust the brightness of the
episcopic illumination. (See Page 42.)
6
Adjust the brightness with ND filters.
(See Page 44.)
5
Set the prism selector knob of the DIC slider to the
position (A or B) indicated on the body of the
objective. (See Page 65.)
6
Rotate the prism position knob at the end of the DIC
prism to set an interference color. (See Page 65.)
You can also perform the sensitive color DIC
microscopy by operating the prism position knob.
7
Adjust the EPI brightness switch on the operation panel to adjust the brightness of the episcopic
illumination. (See Page 42.)
8
Adjust the brightness with ND filters. (See Page 44.)
UEPI2
A
USB
RS23
2C
LCNT
ND8
NCB
F.S.
Achr N.A = 0.9
JAPAN
0.8
0.7 0.6
0.5 0.4 0.3 0.2 0.1
3 x 2 ST
AGE
JAPAN
F
.
STOP
JAPAN
BF
DF FL1 FL2
FL1
FL2
100
20
0
100
IN
OUT
LV-TT
2
1
2
UEPI2A
USB
RS23
2C
LCNT
ND8
NCB
F.S.
Achr N.A = 0.9
JAPAN
0.8
0.7 0.6
0.5 0.4 0.3 0.2 0.1
3 x 2 S
TAGE
JAPAN
F
.
STOP
JAPA
N
BF
DF FL1 FL2
FL1
FL2
100
20
0
100
IN
OUT
LV-TT2
OBJ.
CUBE
A.S.
EPI
DIA
EPI
DIA
6
1
2
4
3
Operation
panel
EPI brightness switch
5
10X/
0.30
A
/0 BD DIC
CF Plan
Set the prism selector
knob to the position
indicated on the objective.
