20
4
Differential Interference Contrast Microscopy under the Epi Illumination (Senarmont Method)
1
Attach the accessories required for the Senarmont method of the DIC microscopy .
The accessories required for the Senarmont method of the DIC microscopy under the episcopic
illumination are as follows:
•
U5AI nosepiece (LV-NU5AI) (See Page 79.)
•
LU objective (See Page 93.)
•
Polarizer slider (equipped with the 1/4 lambda plate) (See Page 83.)
•
Analyzer slider (See Page 83.)
•
Lambda plate slider (for the sensitive color microscopy) (See Page 81.)
•
DIC prism slider (for the sensitive color microscopy) (See Page 83.)
*
For details on selecting the DIC prism, see Page 64, “20. DIC Prism (For the Episcopic Illumination/Senarmont
Method)” of “III. Operation of Each Part.”
*
Set up the microscope according to the "LVSetup" and set information of objectives correctly.
2
Focus on the specimen with the bright-field microscopy under the episcopic
illumination.
(See Pages 15 and 16.)
3
Set the microscope for the DIC microscopy
under the episcopic illumination.
1
Push in the analyzer slider to place the analyzer
into the optical path. (See Page 60.)
2
Push in the polarizer slider to place the polarizer
and the 1/4 lambda plate into the optical path, and
set the crossed Nicols position by aligning the
index. (See Page 57.)
Set the polarizer to the
crossed Nicols position.
3
Press the OBJ. switch on the operation panel to
place the objective of a desired magnification into
the optical path. (See Page 43.)
The aperture diaphragm for the episcopic
illumination is adjusted to the predetermined size
by the interlock control function.
4
Push in the DIC prism slider to place the DIC prism
into the optical path. (See Page 64.)
Methods of the differential interference contrast (DIC) microscopy vary among the nosepiece
configuration. For the standard LV-NU5AI nosepiece, the Senarmont method is used for the DIC
microscopy under the epi illumination.
UEPI2A
USB
RS23
2C
LCNT
ND8
NCB
F.S.
Achr N.A = 0.9
JAPAN
0.8
0.7 0.6
0.5 0.4 0.3 0.2 0.1
3 x 2 ST
AGE
JAPAN
F
.
STOP
JAPAN
BF
DF FL1 FL2
FL1
FL2
100
20
0
100
IN
OUT
LV-TT2
OBJ.
CUBE
A.S.
EPI
DIA
EPI
DIA
Operation
panel
3
4
1
8
OBJ.switch
3
EPI brightness switch
7
2
6
5
