Chapter 1
Microscopy Procedures
7
Chapter 1-1
Micr
oscopy
Pr
oced
ures
Bright-field Microscopy
Microscopy operation
10
Select the desired objective.
Turn the nosepiece to bring the desired objective into the
optical path.
Swing-out condenser 1-100x
Mounting a 1-100x swing-out condenser on the
Ci-L main body when using the 1x objective may
result in uneven illumination around the field of
view.
Bring an arbitrary objective into the optical path.
11
Adjust the aperture diaphragm.
(
→
See Chapter 2,
“6 Adjusting the Aperture Diaphragm” for details)
Turn the aperture diaphragm lever on the condenser to
adjust the aperture diaphragm so that it is set to 70 to 80%
of the numerical aperture of the objective used.
Be sure to adjust the aperture diaphragm each
time you change the objective.
(You can see the aperture diaphragm image with
the centering telescope.)
Right size of the aperture diaphragm
12
Focus on the specimen.
(1) Look into the eyepiece, and adjust the brightness of
the field of view by turning the dia-illumination
brightness control knob. You can also adjust the
brightness with an ND filter for Ci-S.
(2) Rotate the stage knob to move the stage and bring
the target into the optical path.
(3) If the specimen is not in focus, turn the focus knob to
focus on it.
Aperture
diaphragm image
Objective
pupil
70 to 80
100