Chapter 1
Microscopy Procedures
12
Chapter 1-2
Micr
oscopy
Pr
oced
ures
Phase Con
tras
t Mic
ro
scopy
Microscopy operation
(
→
See also: Chapter 2, Section 14 “Tips for Phase Contrast Microscopy”)
11
Bring the Ph annular diaphragm (Ph1) in the condenser turret into the optical path.
Turn the condenser turret until the [Ph1] symbol comes to
the front.
GIF filter
The GIF filter (green interference filter) improves
the contrast when placed in the optical path. The
filter should be installed on the field lens, or
placed inside or on top of the filter cassette holder.
Note, however, that it may cause ghosting when
mounted inside the filter cassette holder.
CLAMP
T
O
RQ
UE
PHASE CONTRAST
0.90 DR
Y
JAPA
N
A
Bring the Ph annular diaphragm into the optical
path.
12
Center the Ph annular diaphragm.
To optimize the phase effect, it is important to properly
overlap the phase plate of the objective with the Ph
annular diaphragm image in the condenser.
(1) Make sure that the 10x objective (Ph1) has been
placed into the optical path and that the [Ph1]
symbol on the condenser turret is facing the front.
(2) Rotate the stage knob to move the specimen and
bring a portion where there is no sample under the
cover glass into the optical path.
(3) Remove one eyepiece from the tube, and insert the
centering telescope with adapter into the tube.
(4) Hold the flange of the centering telescope and rotate
the eyepiece to focus on the phase plate of the
objective.
(5) If the phase plate of the objective and the annular
diaphragm in the condenser are misaligned, loosen
the clamp screws of the two annular diaphragm
centering knobs before rotating the centering knob
to move the entire turret for annular adjustment.
Tighten the clamp screws after the completion of the
adjustment.
(6) Remove the centering telescope and adapter from
the tube, and reattach the eyepiece.
Focusing on the Phase Ring
CLAMP
T
O
RQ
UE
PHASE CONTRAST
0.90 DR
Y
JAP
AN
A
Centering the Ph annular diaphragm
Flange
Eyepiece
Annular
diaphragm
centering knob
Annular diaphragm
centering knob clamp
screw
Condenser
turret
Ph1
Bad Good
GIF filter (green interference filter)
for improving the contrast of a phase image
