Motic Instruments Inc. Canada
October 06, 2006
27
3.18 Changing the size of the field diaphragm
The field aperture diaphragm determines the illuminated area on the specimen.
Operating the field aperture diaphragm lever changes the size of the field aperture
diaphragm. For normal observation, the diaphragm is set just slightly outside the edge of
the field of view.
If a larger than required area is illuminated, extraneous light will enter the field of view.
This will create a flare in the image and lower the contrast.
Cutting out the excessive light is useful in preventing contrast from being diminished.
Decreasing excessive illumination is useful in protecting the specimen from fading.
Fig. 21
Field aperture
diaphragm lever
Epi-fluorescence attachment main body
Shutter slider with
Filter holder
Epi-fluorescence
attachment
main body sleeve