Mirus Ingenio EZporator MTR 51000 Product User Manual Download Page 24

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Mirus Bio LLC

5602 Research Park Blvd., Ste. 210

Madison, WI 53719 USA

U.S. Toll

free: 888.530.0801

Direct: +1.608.441.2852

5423

027 R2.0

Summary of Contents for Ingenio EZporator MTR 51000

Page 1: ...Ingenio EZporator Electroporation System Product User Manual MIR 51000 RevB 121520 mirusbio com...

Page 2: ...ob Pulse Button 10 EZporator System Protocols Quick Reference Guidelines 11 Instructions for Use with Ingenio Solution and Kits 12 13 Optimization Cell Density and Passage Number 14 Nucleic Acid Purit...

Page 3: ...ection efficiency Optimizing parameters such as nucleic acid amount cell density and voltage for each cell type will improve transfection efficiency and decrease cell mortality thus making electropora...

Page 4: ...F capacitance High Voltage HV mode Voltage range of 30 to 2 500 V with 10 V resolution 150 internal resistance and 36 F capacitance Streamlined experimental approach 1 Initialize instrument 2 Set vol...

Page 5: ...rking environments Altitude 2 000 m above sea level operating Mechanical Characteristics Maximum Voltage Output 2 500 V peak Maximum Pulse Length 125 ms at 400 V peak or 5 ms at 2 500 V peak Pulse Wav...

Page 6: ...llow 3 to 5 cm of clearance on all sides of the module for proper cooling GROUND THE PRODUCT This product is grounded through the grounding con ductor of the power cord To avoid electric shock the gro...

Page 7: ...d by Mirus Bio authorized personnel only If there are any questions about the operation of this instrument call Mirus Bio Technical Support at 888 530 0801 or 1 608 441 2852 Caution Notice This symbol...

Page 8: ...insert the power cord plug into an appropriate electrical outlet i e 110 240 VAC To connect the EZporator Cuvette Chamber to the Pulse Generator insert the color coded plugs attached to the Cuvette C...

Page 9: ...0 V 2 V increments 1050 F capacitance HV mode from 20 to 2500 V 10 V increments 6 F capacitance Initializing and Charging Initializing and charging capacitor to the preset voltage level Pulsing Delive...

Page 10: ...separate voltage mode switch Use the Voltage Adjust knob to adjust the voltage from 20 to 400 V in LV mode in 2 V increments and from 30 to 2 500 V in HV mode in 10 V increments If in LV mode increasi...

Page 11: ...fy that the EZporator is in READY mode Then use the Voltage Adjust knob to dial in the appropriate voltage and mode LV or HV for the cell type and buffer to be electroporated NOTE LV mode is used for...

Page 12: ...ypsin EDTA if applicable and complete growth medium to room temperature 2 Harvest and count cells to determine cell density cells ml 3 Determine the total electroporation volume required to perform th...

Page 13: ...t room temperature NOTE The optimal pulse conditions or program settings will vary depending on the cell type and electroporator used For most mammalian and insect cell types optimal conditions fall w...

Page 14: ...s typically between 1 10 106 cells ml Higher cell densities are typically recommended for suspension cells e g 1 x 107 cells ml whereas the recommended range for adherent cells is 1 5 106 cells ml Cel...

Page 15: ...cuvettes Time Constant Time constant TC values will vary depending on electro poration buffer conductivity volume in the cuvette cell density and tempera ture Typical TC values with Ingenio Electropo...

Page 16: ...0 250 150 280 COS 7 0 2 0 4 5 2 5 100 250 150 260 HEK 293 0 2 0 4 5 2 5 100 250 160 250 HEK 293T 0 2 0 4 5 5 250 250 HeLa 0 2 0 4 3 2 5 100 250 130 260 Hepa 0 2 0 4 5 2 100 160 HepG2 0 2 0 4 5 2 5 100...

Page 17: ...or is adequately rated If the message persists contact Mirus Technical Support Setpoint too low The user has entered a setpoint that is outside of normal operating specifications Increase the voltage...

Page 18: ...e If you do not observe expression of your target insert verify the se quence of the plasmid DNA Proper experimental controls were not included for plasmid delivery To verify efficient electroporation...

Page 19: ...ctroporation mix Reduce the amount of DNA used for electroporation DNA concentrations as low as 5 g ml of the final electroporation volume can be used Compare toxicity levels against a cells Ingenio E...

Page 20: ...EZporator fuse is located at the back of the instrument offset below the green Power Button see below Blown fuses can easily be replaced by following these instructions 1 Power off the Pulse Generato...

Page 21: ...you Without limiting the generality of the foregoing Mirus Bio shall not be liable for any claims of any kind whatsoever as to the equipment delivered or for non delivery of equipment and whether or n...

Page 22: ...pent and materials used will be supplied Shipment of your EZporator Device to Mirus Bio should be prepaid Your bill will include return shipment freight charg es Disassembly by the user is prohibited...

Page 23: ...MIR 51000 Notes Mirus Bio LLC www mirusbio com techsupport mirusbio com U S Toll Free 844 MIRUSBIO 844 647 8724 Direct 1 608 441 2852 1996 2021 All rights reserved Mirus Bio LLC All trademarks are th...

Page 24: ...Mirus Bio LLC 5602 Research Park Blvd Ste 210 Madison WI 53719 USA U S Toll free 888 530 0801 Direct 1 608 441 2852 5423 027 R2 0...