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Ingenio® EZporator® Electroporation System
Product User Manual
| Instructions for MIR 51000
Optimization
Pulse Conditions
•
Voltage.
For cells resuspended in Ingenio® Electroporation Solution, the expo-
nential decay pulse conditions for most cell types fall within a voltage range of
80
-
160 V when using 0.2 cm cuvettes (100 µl volume) and 200
-
300 V when
using 0.4 cm cuvettes (250 µl volume). To optimize electroporations, vary the
voltage in 10 V increments (starting at 200 V for 0.4 cm cuvettes and 80 V for
0.2 cm cuvettes).
•
Time Constant.
Time constant (TC) values will vary depending on electro-
poration buffer conductivity, volume in the cuvette, cell density and tempera-
ture. Typical TC values with Ingenio® Electroporation Solution range from 19
-
23
ms in 0.2 cm cuvettes (100 µl volume) and 29
-
32 ms in 0.4 cm cuvettes (250 µl
volume).
Example Voltage Titration:
BJ Fibroblasts were electroporated with a GFP
-
encoding
plasmid DNA at the specified voltages in 0.2 cm cuvettes (100 µl volume). Delivery
efficiency and cell viability were determined by flow cytometry 48 hours post
-
electroporation:
Mirus Bio
LLC
U.S. Toll Free: 844.MIRUSBIO
(844.647.8724)│
Direct: +1.608.441.2852
110 V
130 V
150 V
170 V
