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Page 16
Ingenio® EZporator® Electroporation System
Product User Manual
| Instructions for MIR 51000
Pulse Conditions for Common Cell Types
Mirus Bio
LLC
U.S. Toll Free: 844.MIRUSBIO
(844.647.8724)│
Direct: +1.608.441.2852
Cell Type
Cuvette
Size (cm)
Cell Density
(×10
6
cells/ml)
DNA
(µg)
Electroporation
Volume (µl)
Voltage
(V)
Primary Human
Keratinocyte
0.2
0.4
2
2
5
100
250
150
220
Primary MEF
0.2
0.4
5
2
5
100
250
150
230
Primary Rat
Cortical Neuron
0.2
0.4
1
2
-
100
-
120
-
A549
0.2
0.4
5
-
5
-
250
-
280
BHK21
0.2
0.4
10
2
5
100
250
150
280
CHO
-
K1
0.2
0.4
5
2
5
100
250
150
280
COS
-
7
0.2
0.4
5
2
5
100
250
150
260
HEK 293
0.2
0.4
5
2
5
100
250
160
250
HEK 293T
0.2
0.4
5
-
5
-
250
-
250
HeLa
0.2
0.4
3
2
5
100
250
130
260
Hepa
0.2
0.4
5
2
-
100
-
160
-
HepG2
0.2
0.4
5
2
5
100
250
170
250
HL
-
60
0.2
0.4
10
2
5
100
250
150
276
HUVEC
0.2
0.4
3
-
5
-
250
-
250
Jurkat
0.2
0.4
10
2
5
100
250
150
260
K562
0.2
0.4
10
2
5
100
250
130
250
MCF
-
7
0.2
0.4
3
2
-
100
-
150
-
NIH
-
3T3
0.2
0.4
10
2
5
100
250
160
260
NIKS
0.2
0.4
2
2
5
100
250
170
280
PC
-
12
0.2
0.4
3
2
5
100
250
130
240
RAW 264.7
0.2
0.4
5
2
5
100
250
150
260
SH
-
SY5Y
0.2
0.4
5
-
5
-
250
-
250
SK
-
BR
-
3
0.2
0.4
5
2
5
100
250
160
260
SK
-
N
-
MC
0.2
0.4
5
2
5
100
250
90
240
THP
-
1
0.2
0.4
10
2
5
100
250
140
250
U
-
937
0.2
0.4
10
-
5
-
250
-
260
Vero
0.2
0.4
5
2
-
100
-
170
-
Other
(Starting Point)
0.2
0.4
5
-
10
2
5
100
250
80
-
160
200
-
300