Medonic M16C-US, User Manual

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77
Section 10: Technology
Section Overview
Introduction This section describes the different methods and principles of measurement
and calculations.
Contents This section contains the following topics:
Topic See Page
Measuring Principles 77
Counting Time RBC & WBC 78
WBC Differentials 79
Photometric Method – HGB Hemoglobin 80
Parameter Definitions 80
10.1 Measuring Principles
Description This section describes the measuring principles of the Medonic M-Series.
General
Measuring
Principles
The measuring principles of the Medonic M-Series are based on impedance
and spectrophotometry principles.
Whole Blood
Dilution The number of cells for determining RBC and WBC values are counted from a
suspension of 1:40,000 for the RBC and 1:400 for the WBC dilution ratio of
whole blood.
Theoretical
Principles
(RBC Example) If a sample contains 5 million red blood cells per µl, a dilution of 1:40 000
will give a final concentration of 5 million divided by 40,000 = 125 cells per
µl. Each µl containing 125 cells, drawn through the aperture, will generate 125
pulses.
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