8
Bioscience Solutions
– 4D-Nucleofector™ Manual
2.5 4D-Nucleofector™ System
Components
By definition the 4D-Nucleofector™ System is a modular system offering
maximum flexibility. Therefore, a complete system may vary in the number
and type of components it contains. Furthermore, a system may consist
of minor model variants of the units. These variants can be discriminated
by their part codes and serial numbers (table 2.1).
Table 2.1:
Overview of model variants
Model
Variant A
Variant B
Years
2010 – 2013
2013 – Now
Part codes
Core Unit: AAF-1001B
X Unit: AAF-1001X
Y Unit: AAF-1001Y
Core Unit: AAF-1002B
X Unit: AAF-1002X
Y Unit: AAF-1002Y
LV Unit: AAF-1002L
Serial numbers
Core Unit: 5**B****
X Unit: 5**X****
Y Unit: 5**Y****
Core Unit: 8**B****
X Unit: 8**X****
Y Unit: 8**Y****
LV Unit: 9**LV***
Unit arrangement
horizontal or vertical
(via demountable side panels)
vertical only
(fixed side panels)
Core Unit – display
frame
silver
blue
X/Y Unit – sample rescue
(also see 3.2)
using wheel accessible after
removal of side panel
via screw behind termination
plug
A typical 4D-Nucleofector™ System includes:
1. One 4D-Nucleofector™ Core Unit
2. At least one functional unit
–
X Unit
: comprising two retainers for 100 μL Nucleocuvette™ Vessels
and one retainer for 16-well Nucleocuvette™ Strips
–
Y Unit
: enabling transfection of cells in adherence using a 24-well
Dipping Electrode Array
–
LV Unit:
for large volume transfection of up to 10
9
cells
3. Power cord
4. Interface cable for high voltage and data connection (plus termination
plug cap, not shown)
Figure 2.1:
4D-Nucleofector™ System before assembly
1
2
3
4
5. Touch screen (graphical user interface of the system)
6. Operation LED
7. USB port (for USB stick)
8. X Unit with tray holding retainers for two 100 μL Nucleocuvette™
Vessel and one 16-well Nucleocuvette™ Strip
9. Y Unit with tray holding 24-well Dipping Electrode Array for adherent
transfection
10. LV Unit with slot for 1 mL or LV Nucleocuvette™ Cartridge
Figure 2.2:
4D-Nucleofector™ System comprising Core, X and Y Unit (A) or Core, X and
LV Unit (B)
8
6
7
5
9
10
A
B
