36
Bioscience Solutions
– 4D-Nucleofector™ Manual
Error Code
What Happened?
Possible Error
Procedure
Err1A
No pulse generated
Internal communication failure, internal
error or device possibly defective.
Clear the error message by pressing any button and try to apply
program a second time. If Err1A re-occurs, switch off the device,
check the interface cable, wait for 2 seconds and switch on again.
If error persists, contact our Scientific Support.
Err2
No pulse generated
No or inappropriate cuvette loaded.
Inappropriate Nucleofector™ Solution or
volume.
Check cuvette. Check type and volume of Nucleofector™ Solution.
Cuvette can be reloaded.
Err3
No pulse generated
Improper interconnection or system
defective.
Switch off the device. Check proper connection of the interface
cable as well as the terminator cap on the last interface outlet.
Switch on the device and repeat the experiment with the same
sample.
Err 4 WEAK
A weak pulse occurred. Efficiency may be
sub-optimal.
Re-used cuvette or inappropriate
Nucleofector™ Solution.
Clear the error message by pressing any button. Use the sample
if maximum efficiency is not essential. Otherwise, use a new
sample.
Err7
No pulse generated
No lid detected on the cuvette.
Clear the error message by pressing any button. Check proper
placement of the lid and rerun experiment with the same cuvette.
Err8
Device possibly generated an arc discharge
leading to incomplete program execution.
A substantial reduction in efficiency and
viability must be assumed.
Inappropriate cuvette, Nucleofector™
Solution or volume in the cuvette.
Clear the error message by pressing any button. Check the volume
of Nucleofector™ Solution. Try again with a new cuvette.
Err11
No pulse generated.
The device case is open.
Switch the device off, check the correct mounting of all side
panels and the interlock between all modules. Try again with the
same samples. Can only occur with model variants A (see 2.5).
Err12
Pulse possibly omitted.
Internal device temperature too high for
pulsing.
Switch off the device, wait for 2 minutes and run experiment with
a new cuvette.
Err13
No pulse generated.
The device front flap is open.
Check proper closing of front flap after the tray is closed and rerun
the experiment.
Err 21
Used well:
Multiple use of well. Nucleofection may be
suboptimal.
Dipping electrode for that well position was
used twice.
Utilize sample if maximum performance is not essential. Avoid
re-use of dipping electrodes that had been used before.
Err 22
Used well:
A weak pulse occurred. Efficiency may be
sub-optimal.
Re-used dipping electrode or inappropriate
Nucleofector™ Solution.
Clear the error message by pressing any button. Use the sample if
maximum efficiency is not essential. Otherwise, use a new sample.
Err 23 & Err24
Used well:
Device possibly generated an arc
discharge. Program could be resumed
and successfully completed. A limited
impairment on performance has to be
presumed.
Inappropriate solution or well volume,
occasional arcing.
Utilize sample if maximum performance is not essential.
Check solution and volume.
Err 25
Used well:
Device possibly generated an arc discharge
leading to incomplete program execution.
A substantial reduction in efficiency and
viability must be assumed.
Inappropriate dipping electrode,
Nucleofector™ Solution or volume in
the dipping electrode. Device possibly
defective.
Clear the error message by pressing any button. Check the volume
of Nucleofector™ Solution. Try again with a new dipping electrode.
Err 26
Used well: Multiple use of well
Nucleofection suboptimal
Dipping electrode for that well position was
used multiple times.
Utilize sample if maximum performance is not essential. Avoid re-
use of dipping electrodes that had been used before.
Res 27
Used well:
Well was skipped
User confirmed to skip the well as it had
been used before
–
Err 30
Cartridge not detected
Cartridge not properly inserted/arrested
Ensure proper interlocking of cartridge. Remove and insert
cartridge again and check snapping of lower and upper release
lever.
Err 31
No liquid was detected at liquid sensor 1
(upper sensor)
Cell suspension reservoir not filled or not
attached.
Pump not closed.
Liquid in reservoir too viscous.
Check correct filling and attachment of reservoirs.
Close pump.
Err 32
No liquid was detected at liquid sensor 2
(lower sensor)
Substrate reservoir not filled or not
attached.
Pump not closed.
Liquid in reservoir too viscous.
Check correct filling and attachment of reservoirs.
Close pump.
Err 33
Target volume not reached for reservoir 1
(upper pump)
Cell suspension reservoir empty before
the entered volume has been completely
processed.
Bubbles in tubing.
Check if reservoir is empty. Check if filling should have matched
entered values.
Reduce speed of magnetic stirrer.
Do not re-use consumables.
Err 34
Target volume not reached for reservoir 2
(lower pump)
Substrate reservoir empty before the
entered volume has been completely
processed.
Bubbles in tubing.
Check if reservoir is empty. Check if filling should have matched
entered values.
Do not re-use consumables.
