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Instructions for Use

Leica ST4020

Small Linear Stainer

Leica ST4020, English

Order No.:

 14 0509 80101 RevE

Always keep this manual with the instrument.

Read carefully before working with the instrument.

1.2 RevE - 08/2016

Summary of Contents for ST4020

Page 1: ...ions for Use Leica ST4020 Small Linear Stainer Leica ST4020 English Order No 14 0509 80101 RevE Always keep this manual with the instrument Read carefully before working with the instrument V 1 2 RevE...

Page 2: ......

Page 3: ...echni cal details of the present manual are not to be considered as warranted characteristics of our products These are determined only by the contract pro visions agreed between ourselves and our cus...

Page 4: ...tallation site requirements 17 4 2 Unpacking the instrument 17 4 3 Setup 19 4 4 Water Connection 20 4 5 Installing the rinsing water containers 20 4 6 Remedy if hose is too short 24 5 Operation 25 5 1...

Page 5: ...Troubleshooting 40 7 1 General 40 7 2 Alarms 40 7 3 Fault detection and correction list 41 7 4 Power failure 43 8 Optional Accessories 44 8 1 Ordering information 44 9 Warranty and Service 50 10 Deco...

Page 6: ...is used for the automated preparation of routine histo logical and cytological stains It is intended for use in pathology laboratories to assist with the following tasks Staining thin tissue sections...

Page 7: ...instrument To ensure trouble free operation of the instrument make sure to comply with the following instructions and warnings For current information about appli cable standards please refer to the...

Page 8: ...socket Do not interfere with the ground ing function by using an extension cord without a ground wire The instrument will automatically detect the voltage frequency of the power source The installati...

Page 9: ...d accessories may cause hazards damage the instrument and void the warranty The instrument may be operated by trained laboratory personnel only It must only be oper ated for the purpose of its designa...

Page 10: ...08 2016 3 1 Overview instrument components 3 Instrument Components and Specifications Fig 2 Adjustable Foot Reagent Container Specimen side carrier Lift Bar Hood optional Rinsing Water Container Disp...

Page 11: ...in staining samples from either Mohs technique laboratories or sections from frozen section laboratories or general surgical pathology In addition it is intended to be used in hematology cytology as w...

Page 12: ...13 Slide Carrier 1 and Lift Bar 2 Reagent container 4 and rinsing water container 6 The user inserts the slides for staining 3 in the slots of the slide carrier 1 This is then placed onto the Lift Bar...

Page 13: ...ent can be operated with alternating current at 100 to 230 Volt with a power frequency of 50 60 Hz A connection port for the rinsing water supply is located on the rear of the stainer The necessary co...

Page 14: ...Lift Bar Retainer Side Support Slides Fig 12 and Fig 13 show how the slide carriers are to be positioned over the reagent containers While placing a slide carrier with slides for staining in the Lift...

Page 15: ...09 46532 1 Water drain hose 150 cm long installed on the instrument 14 0509 46445 1 Hose distributor to rinse stations 1 8 installed in the instrument 14 0509 46533 1 Exit Tank 14 0509 46450 1 Set of...

Page 16: ...ent stations are possible Reagent container volume 50 ml Agitation 0 to 3 dips per station for dwell times 4 sec Throughput rate of wash stations 250 ml min 100 ml dependingonnumberofstations Dwell ti...

Page 17: ...floor Avoid vibrations direct sunlight and heavy variations in temperature 4 Instrument Setup Open the packaging Remove foam material Take out all accessories and the instructions for use Check the co...

Page 18: ...cessory carton 37 and remove the packing material 39 4 Use the molded foam pads 38 to pull the instrument out of the inside packaging and place it on a firm laboratory bench 5 Pull the molded foam pad...

Page 19: ...ru ment is in a stable position at the installation location according to requirements Retighten the locknuts 4 3 Setup Mounting the Support Bracket To ensure that the instrument cannot tip over when...

Page 20: ...4 4 5 Installing the rinsing water containers Up to three rinsing stations may be provided on the stainer The rinsing water containers can be located at any of the 14 reagent stations The equipment s...

Page 21: ...y do not connect a water supply line to the instrument Place the rinsing water con tainers 6 in the desired posi tion on the platform Start with the station closest to the drain age tank Insert the ri...

Page 22: ...end of the connector and make a second marking Marking 2 to indicate the lateral surface of the connector Fig 25 Cut the hose off at Marking 2 Then push the hose 21 fully onto the connector by twist i...

Page 23: ...rly Repeat the steps described above for the other rinsing stations Installing the rinsing water containers continued If only one or two rinsing stations are required the hoses that are not used must...

Page 24: ...again and if necessary cut it to exactly the required length Push both ends of the hose as far as they will go onto the extension con nector and then secure each hose with a cable tie 18 Finally chec...

Page 25: ...on the rear of the instrument is in the OFF 0 position Insert the correct power cord into the power input socket and connect to a power socket 12 If applicable switch on the switch for the power sock...

Page 26: ...increases the displayed value and MINUS reduces the displayed value keys respectively Pressing the PAUSE STOP key allows the operator to exit the setup menu without changing the1 parameter currently b...

Page 27: ...ill take effect immediately Furthermore since all settings are saved in memory it will automatically be used the next time the system is powered up The system returns to its idle mode whenever a new s...

Page 28: ...ps is stored Also the transfer mechanism advances to the next station stopping when the selected number of steps has been taken We recommend not altering the value adjusted at the factory Number of Di...

Page 29: ...tion as posi tion 5 the user allows the system to issue its reminder beep at the correct time Adjustment takes place using the PLUS or MINUS key After ENTER is pressed the selected start position is s...

Page 30: ...vates Now slowly open the water tap to which the supply hose is connected Adjust the flow rate so that a sufficient amount of rinsing water flows into the rinsing water container and the rinsing water...

Page 31: ...result in improper or incomplete pro cessing of slides The slide carrier must be prepared carefully becauseiftheLiftBarispositionedincorrectly it may not transport the slides properly There are two o...

Page 32: ...ing the number of steps transfer mechanism calibration will cause the transfer mechanism to advance to the next position All parameters should be verified BEFORE loading the first set of slides A stai...

Page 33: ...ers must be inserted at the start position immediately and the RUN key pressed As soon as the RUN key is pressed the system resumes process ing and continues counting down the process time Please wait...

Page 34: ...ers af ter processing has begun and must be followed under all circumstances Attempting to add slide carriers without pressing the PAUSE and RUN keys will result in the carriers not being transported...

Page 35: ...is is then the same situation as when the instrument is first switched on The corresponding message appears in the display As soon as the transfer mechanism reaches the start position the stainer retu...

Page 36: ...carriers This enables the operator to remove the slides at a more convenient time As soon as there are 3 slide carriers in the exit tank the stainer emits a distinctive audible signal 5 beeps to aler...

Page 37: ...lowing ways Removing processed slides continued 1 To remove stained slides only Take ALL of the slide carriers out of the instrument s exit tank Press the RUN key once to start processing the loaded s...

Page 38: ...o come into contact with the electrical connections or get inside the instrument 6 1 Cleaning the Instrument Instrument interior Clean with domestic or laboratory cleaning agents as necessary The oute...

Page 39: ...e personnel may repair the instrument and access the instrument s internal components For your safety never attempt to repair the instrument yourself Unauthorized repairs will render any claims under...

Page 40: ...rs automatically If an operation error is detected the transfer mechanism will first stop moving It will then back up a short distance stop again and then move forward in a second attempt to reach the...

Page 41: ...text is visible The contrast may have to be adjusted again The control board in the in strument is defective Adjust the contrast as de scribed in Chapter 5 3 Inform Technical Service Unit powers up b...

Page 42: ...e to the stainer and the tap Turn on the tap Inform Technical Service Replace flow restrictor Water overflows at the rinse stations Tap is open too far The drain hose is clogged The drain hose is rout...

Page 43: ...fuse always switch the instrument off and unplug from power supply Defective fuses may be replaced only with the replacement fuses supplied together with the instrument Before plugging the power cabl...

Page 44: ...e connector in line filter 4 hose clamps and 2 brass fittings 1 52 m long and 0 64 cm reinforced hose 14 0509 46443 Hose for water inlet with hose connector 2 hose clamps 12 5 cm longer and 0 64 cm re...

Page 45: ...Order No 14 0509 46437 48 in a pack Order No 14 0509 46439 Slide Carriers 4 in a pack Order No 14 0509 46438 48 in a pack Order No 14 0509 46440 Wash Station Consisting of 1 container 1 cleaning nozzl...

Page 46: ...Tubing and Fitting Kit 1 22 m hose for wash station 0 32 cm 2 Y fittings 3 in line pipe unions 2 plugs 10 cable ties Order No 14 0509 46459 Fig 37 Fig 38 Water Inlet Hose Assembly with hose connector...

Page 47: ...64 cm rein forced hose no in line filter Order No 14 0509 46532 Fig 40 Drain hose 1 52 cm long 1 6 cm 0 32 cm cable tie Order No 14 0509 46445 Fig 42 Fig 41 Adapter kit for Water Inlet Hose Connection...

Page 48: ...RevE 08 2016 Fig 43 Support Bracket Order No 14 0509 46570 Fig 44 Stabilizer Bar Order No 14 0509 46451 Exit Tank Order No 14 0509 46450 Fig 45 Fig 46 Reagent Container Cover metal Order No 14 0509 4...

Page 49: ...las Hood Order No 14 0509 46478 Fig 47 8 Optional Accessories Packaging with foam outer carton bubble wrap and accessory box with packaging instructions Order No 14 0509 46455 Outer Carton Only Order...

Page 50: ...luded agree ment The warranty terms of your Leica sales organization or the organiza tion from which you have purchased the contractual product shall apply exclusively Service information Ifyouareinne...

Page 51: ...ntamination documentation If you have any further questions please call your local Leica organization Leica Internal Use If applicable note corresponding Job and RAN RGA Number Job Sheet No __________...

Page 52: ...never possible please use the original transportation case box Yes No 3 2 Signature Date Name Position eMail Institute Department Address Phone Fax Important to avoid refusal of shipment Place one cop...

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Page 54: ...www LeicaBiosystems com Leica Biosystems Nussloch GmbH Heidelberger Strasse 17 19 D 69226 Nussloch Phone 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems com...

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