Page 38 Operating Manual EM CPD300 12/18
8.5
Filling the CO
2
into Sample Chamber
Wait until the sample chamber temperature has reached 15°C.
Once this temperature is reached, completely open the shut-off valve of the CO
2
bottle. Press CO
2
in with the selected speed (slow, medium, or fast).
The pressure display shows the pressure in the sample chamber (identical to the
pressure in the CO
2
bottle).
Fill the sample chamber up to the upper edge of the front viewing glass with liquid
CO
2
.
Press the CO
2
-In button again. CO
2
goes off. The CO
2
level in the sample chamber
may rise slightly.
8.6
Mixing the two liquid media
Press the STIRRER key. The magnetic stirrer is switched on. The magnetic stirrer
should not be used if the specimens are very delicate.
8.7
Exchange media mixture from Sample Chamber
Press the EXCHANGE button. Drain the media mixture from the sample chamber
until the specimen is barely covered by the liquid.
Press the EXCHANGE button again to close.
This procedure of filling, mixing, and draining needs to be repeated several times
(minimum 12 times) before heating to remove all the exchange fluid from the
specimens. Multiple and, or larger specimens require more changes.
Fill up to here
with liquid C0
2
Summary of Contents for EM CPD300
Page 1: ...From Eye to Insight OPERATING MANUAL EM CPD300 167180032 Version 12 18 ...
Page 30: ...Page 27 Operating Manual EM CPD300 12 18 Press Start to activate program Test ...
Page 48: ...Page 45 Operating Manual EM CPD300 12 18 After software update press back ...
Page 52: ...Page 49 Operating Manual EM CPD300 12 18 Remove poral filter with spring from the nut ...
