5. OPER ATION - TR ANSMIT TED LIGHT CONTR AST ME THOD
5.13.6 Integrated Phase Contrast – IPH (TL)
Note:
The Integrated Phase Contrast requires a condenser on the
tiltable Transmitted Light arm.
Leica DMi8 with manual Transmitted Light method
t *GOFDFTTBSZTFUUIF5-#SJHIUmFMEQPTJUJPOPSBOFNQUZ
position on the fluo turret, or select the "DAPI-TL" filter
cube.
t 4FMFDUB#SJHIUmFMEPCKFDUJWFXJUIFZFQPJOU#PS$
t4FMFDUUIFDPSSFTQPOEJOH
light ring on the condenser (see
table).
t$PNQMFUFMZPQFOUIFBQFSUVSFPOUIFDPOEFOTFS
t 4XJOHBMMSFNBJOJOHPQUJDBMDPNQPOFOUTTVDIBTUIFBOB
-
lyzer, polarizer or IC prisms, from the beam path.
t *GOFDFTTBSZSFNPWFUIFDPWFSPGUIFIPMEFSBOEJOTFSU
the Phase Contrast module evenly from the front on the
left side of the microscope.
t 4MJEFUIF1IBTF$POUSBTUNPEVMFJOUPUIFDPSSFDUFZFQPJOU
B or C.
t*OTFSUB1IBTF$POUSBTUTQFDJNFO
t'PDVTUIFJNBHFVTJOHUIFGPDVTEJBMT
t$POmHVSFUIFCSJHIUOFTTPOUIFJOUFOTJUZSPUBSZLOPC
IP0
for 5x
e.g. NPlan 5x
Objective with eyepoint B
IP1
for 10x
e.g. NPlan 10 x
Objectives with eyepoint B and
for 20x
e.g. NPlan L 20 x
Objectives with eyepoint C
IP2
for 40x
e.g. HCX PL FL L 40 x
Objectives with eyepoint C
IP3
for 63x
e.g. PL FL 63x/0.70
Objectives with eyepoint C
Phase Contrast module for tiltable Transmitted Light illumi-
nation carrier
1 Centering screws for light ring 20x - 63x
2 Position for objectives 20x - 63x
3 Brightfield position
4 Position for objectives 5x - 10x
5 Phase ring 20x - 63x
6 Phase ring 5x - 10x
7 Fastening screw for light ring 20x - 63x
Receptacle for Phase Contrast module
1
2
3
4
5
6
7
76
Summary of Contents for DMi8
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