Leica DMi8 Instructions For Use Manual Download Page 76

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5. OPER ATION - TR ANSMIT TED LIGHT CONTR AST ME THOD

5.13.5 Differential Interference Contrast – DIC (TL)

Leica DMi8 with manual Transmitted Light method

t *GOFDFTTBSZTFUUIF5-#SJHIUmFMEQPTJUJPOPSBOFNQUZ

position on the fluo turret, or select the "DAPI-TL" filter
cube.

t4FMFDUBOPCKFDUJWF

t4FMFDUUIFDPSSFTQPOEJOH8PMMBTUPOQSJTNDPOEFOTFSPO

the condenser.

t 4FMFDU UIF DPSSFTQPOEJOH 8PMMBTUPO QSJTN PCKFDUJWF PO

the objective nosepiece.

t 4XJOHUIF

polarizer on the condenser into the beam path.

t 5IFOJOTFSUUIF

analyzer on the right side of the stand un-

til it locks into place.

t *OTFSUBTQFDJNFO

t'PDVTUIFJNBHFVTJOHUIFGPDVTEJBMT

t$POmHVSFUIFCSJHIUOFTTPOUIFJOUFOTJUZSPUBSZLOPC

t 'PS mOF BEKVTUNFOU VTF UIF BEKVTUJOH TDSFX CFMPX UIF

objective nosepiece.

Setting objective prism

Fine adjusting the
objective prism

Condenser setting for tiltable Transmitted Light arm - con-
figuring Wollaston prism

Swinging in polarizer

Summary of Contents for DMi8

Page 1: ...Instructions for Use t Gebrauchsanweisung t Mode d emploi Leica Microsystems CMS GmbH t Instructions 11934056 Revision 1 1 from 2015 07 15 Leica DMi8 ...

Page 2: ...2 COPYRIGHT CONTENTS ...

Page 3: ...Instructions for Use Leica Microsystems CMS GmbH t Instructions 11934056 Revision 1 1 from 2015 07 15 Leica DMi8 ...

Page 4: ...al safety notes 8 3 2 Electrical safety 8 3 3 Laser safety 11 3 3 1 Safety switch interlock on the microscope 11 3 3 2 Screwed on filter turret cover 12 3 3 3 Laser coupling by the user 12 3 3 3 1 Safety hazard due to folded back Transmitted Light arm 13 3 3 3 2 Safety hazard at eyepiece 13 3 3 3 3 Safety hazard in the condenser area during operation 13 3 3 3 4 Safety hazard above the condenser 14...

Page 5: ...7 Focusing 54 5 7 1 Focusing the image 54 5 7 2 Focus thresholds 55 5 7 3 Adjusting the step sizes 56 5 7 4 AFC Adaptive Focus Control 57 5 8 Selecting the ports 58 5 9 Magnification Changer 59 5 10 Aperture diaphragm and field diaphragm 60 5 11 Filter 62 5 11 1 Transmitted Light filter 62 5 11 2 Fluorescence Cubes 63 5 11 3 Leica RFID Module 65 5 11 3 1 Product Description 65 5 11 3 2 Specificati...

Page 6: ...r or moderate injury Caution In this manual additional safety notes are indicated with the triangle symbol shown here and have a gray back ground Caution The instrument and accessories can be damaged when operated incorrectly Caution These Instructions for Use are an essential component of the product they must be read carefully before the product is assembled put into operation or used and must b...

Page 7: ...etic field Warning of UV radiation Wear safety goggles Warning of hazardous laser radiation take appropriate safety precautions Warning of hand injuries Instructions for disposing of the instrument its acces sories and consumables Connection to ground Explanatory note Item not contained in all equipment configurations Manufacturing date e g 11 2011 for November 2011 China RoHS 50 years EFUP Enviro...

Page 8: ...Community EC Directives The named microscope meets the requirements of the Council Directives 2006 95 EC and 2014 35 EC concerning electrical apparatus and 2004 108 EC and 2014 30 EC con cerning electromagnetic compatibility Reasonably foreseeable misuse The following are prohibited t To use the microscope for any purpose not in accordance with the Declaration of Conformity e g the use of medical ...

Page 9: ...ices In the standard design without additional laser safety precautions the microscopes are not suitable for cou pling laser radiation such as to the camera ports since this radiation poses a hazard for the user eye injury in particular For use of the microscope with lasers Leica Microsystems CMS GmbH offers special micro scope variants with additional safety features Laser couplings require corre...

Page 10: ...Caution The instruments and accessories described in this In structions for Use have been tested for safety and po tential hazards The responsible Leica Microsystems CMS GmbH af filiate or the main plant in Wetzlar must be consulted whenever the device is altered modified or used in con junction with non Leica components that are outside of the scope of this manual Unauthorized alterations to the ...

Page 11: ...n class I Overvoltage category II Pollution degree 2 Leica DMi8 automated For indoor use only Supply voltage 100 240 VAC o Leica CTR Frequency 50 60 Hz o Leica CTR Power consumption see Leica CTR Fuses see Leica CTR Ambient temperature 15 35 C Relative humidity 90 up to 30 C non condensing Protection class I o Leica CTR Overvoltage category II o Leica CTR Pollution degree 2 o Leica CTR Leica EL600...

Page 12: ...an cause electric shock Caution Protect the microscope from excessive temperature fluctuations Such fluctuations can lead to the accumu lation of condensation which can damage the electrical and optical components Operating temperature 15 35 C WARNING Before exchanging the fuses or lamps be absolutely certain to switch off the main power switch and remove the power cable Caution Only insert or unp...

Page 13: ... equivalent interlock connectors which lead to the microscope s interlock switch are attached to the back of the microscope The interlock connector of the microscope must be connected to your laser beam interrupter Interlock connectors on the rear side of the stand Technical data for the interlock connector Plug Manufacturer Binder Type 09 0097 20 05 5 pin flange plug 711 series subminiature round...

Page 14: ...te system We do not make provisions for your interference with the device and therefore you are fully responsible for the modifications regarding the adherence to basic safety re quirements and laser protective measures set forth by the European Union guidelines and by the national legal and technical directives and standards If you modify your microscope by coupling a laser beam without authoriza...

Page 15: ...n disperse from the eyepiece and directly hit the user s eye Important safety note As a safety measure we recommend the microscope models with integrated interlock connectors as men tioned in Section 3 3 3 7 3 3 3 3 Safety hazard in the condenser area during operation If you couple a high power laser beam into the microscope the laser radiation broadened by the lens that passes the condenser later...

Page 16: ... and the illuminated field diaphragm is without obstruction 3 3 3 5 Safety hazard in the specimen area The laser radiation is accessible without obstruction in the specimen area of the microscope Laser beam path between condenser base and illuminated field dia phragm without obstruction Specimen area 14 ...

Page 17: ...icroscope are tightly sealed and can only be opened with tools to prevent laser radiation from escaping The openings to consider for example top port next to the eyepieces left and right side port bottom port infinity port connection for Transmitted Light lamp housing at the arm lamp connection for fluorescence connection for incident light lamp housing cover for fluorescent turret disk The materi...

Page 18: ... No 11525105 with short cable or order No 11525116 with long cable is not suitable for laser applications and therefore is not provided for these applications a Safety function for the safety hazard mentioned in Section 3 3 3 1 The special microscope variants have a built in interlock switch on the Transmitted Light arm that is activated when folding back the Transmitted Light arm interlock switch...

Page 19: ...led into the microscope cannot get into the user s eyes through the eyepieces Important safety note In addition observe the safety hazards described in Section 3 3 3 3 to 3 3 3 6 3 3 4 Exception for Class 1 lasers Coupling a laser of Class 1 in accordance with IEC EN 60825 1 into a microscope does not result in any danger to the user The listed safety hazards apply only for lasers of Class 1M 2 2M...

Page 20: ...UVSSFU DPWFS F H XJUI adhesive tape t 3FNPWF BMM NPWJOH PS MPPTF QBSUT t 1SPUFDU UIF NJDSPTDPQF GSPN EVTU BOE EJSU VTJOH B QMBTUJD bag Weight CAUTION At a full load the microscope weighs more than 18 kg The user must take corresponding precautions for transporting the instrument Caution For transport always remove all components listed un der Transport 3 6 Ambient conditions Caution This microscop...

Page 21: ...ay from the wall and from flammable substances 3 7 Notes on handling light sources CAUTION Light sources pose a potential irradiation risk glare UV radiation IR radiation Therefore lamps may only be operated in closed housing and in an installed condition Never look directly into the beam path blinding hazard CAUTION Do not switch on the light source until the light guide is connected to the micro...

Page 22: ...n tact Ask the supplier for a safety data sheet 3 10 Notes on handling acids and bases For examinations using acids or other aggressive chemi cals particular caution must be taken Caution Be absolutely certain to avoid direct contact with these chemicals 3 11 Disposal Once the product has reached the end of its service life please contact Leica Microsystems GmbH Service or Sales about disposal Ple...

Page 23: ...rview 4 1 Microscope configurations examples Leica DMi8 with at least one motorized component Leica DMi8 with encoded components Leica DMi8 with manual components With a fixed Transmitted Light arm Leica DMi8 with manual components With a tiltable Transmitted Light arm 21 ...

Page 24: ...4 OVERVIEW OF THE INSTRUMENT 4 2 Dimensions specified in mm Leica CTR compact 195 204 130 168 1 162 5 Weight approx 2 kg Leica CTR Advanced Advanced 326 275 6 310 215 270 Weight approx 4 kg 22 ...

Page 25: ...prox 19 kg Height compensation plate In order to enlarge the viewing height by 23 mm raise the side camera ports for very large cameras or spinning disks or to use a microscope with an inactive bottom port even without a hole in the work table a height compensation plate order No 11525200 was developed 23 ...

Page 26: ... SPPN GPS TQFDJNFOT BOE micromanipulators t PS S40 condensor with an integrated manual or motorized illuminated field dia phragm t JMUFS NBHB JOF GPS SFQMBDFBCMF mMUFST t 8JUI DPOEFOTFS RVJDL DIBOHF t BOVBM TIVUUFS t BNQ IPVTJOH SFDFQUBDMF GPS SFQMBDFBCMF T t 8JUI JOUFHSBUFE DBCMF EVDU t POEFOTPS TUPQ UP QSFWFOU GSPN CFJOH QVTIFE EPXO BDDJEFOUBMMZ or Fixed Transmitted Light arm t With integrated L...

Page 27: ...S MJHIU HVJEF BEBQUFS t PUPSJ FE nVPSFTDFODF JOUFOTJUZ NBOBHFS Reduction of light intensity from the incident light illumination Infinity port and T Houses t 8JUI B NJSSPS GPS TXJUDIJOH CFUXFFO UIF MFGU PS SJHIU BOE UIF SFBS QPSUT or t 8JUI QSJTN With 2 possible positions 50 rear port 50 left right port 80 rear port 20 left right port 100 rear port 0 left right port Additional T Houses 1 No fluore...

Page 28: ...PTJUJPO GPS PCKFDUJWFT XJUI UISFBET BOE B NN QBSGPDBMJ JOH EJTUBODF t For DIC manual Wollaston prism turntable Stages t JYFE TUBHFT t 4UBHF QMBUF DPBUFE JO DFSBNJD NN Y NN t Heated stage plate 3 C above room temperature up to 60 C 248 x 212 mm t 5FNQFSBUVSF BEKVTUBCMF TUBHF QMBUF UP NN Y NN t JYFE NJDSPNBOJQVMBUJPO TUBHFT t 4UBHF QMBUF DPBUFE JO DFSBNJD NN Y NN t FBUFE TUBHF QMBUF BCPWF SPPN UFNQF...

Page 29: ...NN BCPWF UIF TUBHF t BYJNVN USBWFM TQFFE NN T t GPDVT JODSFNFOUT N N N N N t MFDUSPOJD GPDVT SFQPTJUJPOJOH t VUPNBUJD MPXFSJOH CFGPSF PCKFDUJWF DIBOHF t MFDUSPOJD QBSGPDBMJUZ t 0QUJPOBM EBQUJWF PDVT POUSPM t 0QUJPOBM MPTFE PPQ SFQPTJUJPOJOH BCJMJUZ 20 nm t BOEXIFFMT FBDI XJUI JODSFNFOUT GPS DPBSTF BOE mOF GPDVTJOH JODSFNFOUT Leica DMi8 manual t BOVBM t NN USBWFM QBUI NN CFMPX NN BCPWF UIF TUBHF t ...

Page 30: ...PS PQFSBUJOH UIF NPUPSJ FE TIVUUFS t WBSJBCMF GVODUJPO LFZT t PQFSBUJOH CVUUPOT GPS GPDVT UISFTIPMET POMZ GPS FMFDUSPOJD GPDVTJOH t IBOEXIFFMT GPS GPDVTJOH t 3PUBSZ LOPC GPS TFUUJOH UIF CSJHIUOFTT t CVUUPOT GPS nVPSFTDFODF DVCFT t CVUUPOT GPS PCKFDUJWFT t FJDB 4NBSU PWF FSHPOPNJD DPOUSPM FMFNFOU GPS NPOJUPSJOH Y Z BOE BEEJUJPOBM WBSJ able function keys t FJDB 451 PQFSBUJPO WJB FYUFSOBM 5PVDI 4DSFF...

Page 31: ... 1 2 3 4 5 6 Maximum system design of the left control panel field The number of buttons may vary depending on the configu ration Control panel field on the right microscope side 1 Variable function keys freely configurable The function keys are preassigned at the factory see Identification Sheet and S 92 2 Focus threshold the LED illuminates when the focus threshold is set 3 Setting the focus pos...

Page 32: ...rized objective nosepiece and motorized fluo turret 1 6 filter cube changer buttons with LEDs for indicating the current filter 2 Label fields 3 6 objective changer buttons with LEDs for indicating the current objective Encoded objective nosepiece and encoded fluo turret 1 6 LEDs for indicating the filter cube 2 Label fields 3 6 LEDs for indicating the current objective 3 1 2 1 1 2 3 1 2 Stickers ...

Page 33: ...entering 6 Toggling the top port and Bertrand lens 7 Manual objective change 8 Left side port 9 Manual filter change 10 Focusing 11 Brightness adjustment 12 IMC module Left side of the Leica DMi8 with a tiltable Transmitted Light arm 1 Illuminated field diaphragm adjustment motorized or manual 2 Condenser height adjuster 3 Swinging in the polarizer 4 Aperture Diaphragm Adjustment 5 Condenser setti...

Page 34: ...ltable Transmitted Light arm 1 Switching the Transmitted Light filter on and off The front position is assigned at the factory using a shutter dark stop which is interchangeable with a fil ter 2 Tube setting 3 Toggling the infinity port 4 Analyzer slot 5 Identification label 6 Right side port 7 Cover for fluorescent turret disk 8 Focusing 9 Focus thresholds 10 Variable function keys 1 3 2 4 6 7 8 ...

Page 35: ...only on manual stands 8 Illuminated field diaphragm adjustment 9 Manual setting for FIM 10 Centering illuminated field diaphragm 11 Manual shutter 3 2 1 4 5 7 8 9 10 11 6 Manual external fluorescence axis Alternative connection on the left or rear microscope side 1 Connection for light guide 2 FIM 3 Diaphragm module 4 Receptacle for EFW or structured illumination 5 Manual Shutter 1 2 3 5 4 10 9 8 ...

Page 36: ...levels of operation Level 1 Navigation bar Level 2 Menu bar Level 3 Control panel field When you select a pictogram in the navigation bar on the left hand border of the display the corresponding menu bar on the top border of the display is shown The display on the control panel changes accordingly For some func tions an additional information field appears above the op erating keys The navigation ...

Page 37: ...ing the microscope settings In Microscope Settings press Save to write the cur rent microscope settings to a USB stick inserted into the left side of the microscope Service port Pressing Load restores all settings Display brightness To prevent unwanted interference from light you can switch off the display using the Switch off key To switch it back on simply touch any location on the display You c...

Page 38: ...ccordingly For details on key as signments please refer to the included identification sheet For information on the abbreviations used please refer to the list o S 94 Note The key assignment can be changed using the LAS X soft ware Note To lock the controls press and hold the two front keys 5 simultaneously for 5 seconds Repeat this process to unlock the controls The status LED 6 indicates whether...

Page 39: ... 4 4 5 Leica STP4000 8000 external control panel The Leica STP4000 enables remote control of all automated microscope functions using an external Touch Screen The Leica STP8000 control panel also enables monitoring in X Y and Z and has user definable function keys Separate instructions are provided for operation of the Leica STP4000 8000 Navigation bar Menu bar Control panel Touch Screen for STP40...

Page 40: ...ens surfaces on the objective If fingerprints do appear on the glass surfaces remove them with a soft leather or linen cloth Even small traces of finger perspiration can damage the surfaces in a short time For additional instruc tions see the chapter entitled Care of the Instrument o S 89 Note Operating the motorized components using the Leica STP4000 8000 control panel is described in separate In...

Page 41: ...motorized component and Leica CTR electronics box t 4XJUDI PO UIF FMFDUSPOJDT CPY PO UIF PO PGG TXJUDI 5IF indicator lamp is illuminated in green during operation Note If you have connected a PC switch on the electronics box first and then the computer All motorized microscope components will then run through an initialization phase Note At initialization all of the user s most recent settings are...

Page 42: ...t source on the on off switch The indicator lamp is illuminated during op eration CAUTION Do not switch on the light source until the light guide is connected to the microscope Uncontrolled light output from the light guide poses a blinding hazard There is also a burn hazard at the light guide output Caution When using other light sources observe the respective instructions for the light source 40...

Page 43: ...nual shutter or press the shutter button for motorized shutter t 4FU UIF light intensity using the knob on the left side of the stand t G UIF NJDSPTDPQF IBT B NBOVBM TIVUUFS BOE B TIVUUFS CVU ton the light is switched off when pressing the button To switch it on again press the rotary knob for the light in tensity t 4FU UIF MJHIU JOUFOTJUZ VTJOH UIF LOPC PO UIF MFGU TJEF PG UIF stand Leica DMi8 wi...

Page 44: ...ored for each objective and contrast method 5 2 2 Fluorescence Leica DMi8 with manual encoded fluorescence t G OFDFTTBSZ mSTU TXJUDI PWFS UP UIF nVPSFTDFODF BYJT Fluor by opening the shutter pull out switch rod at the manual integrated fluorescence axis or on the manual ex ternal fluorescence axis t 4FU UIF MJHIU JOUFOTJUZ PO UIF Leica EL6000 compact light source See separate Instructions for Use ...

Page 45: ...fluorescence t If necessary switch over to the fluorescence axis Fluor with the TL Fluor button t 4FU UIF MJHIU JOUFOTJUZ PO UIF Leica EL6000 compact light source See separate Instructions for Use CAUTION Do not switch on the light source until the light guide is connected to the microscope Uncontrolled light output from the light guide poses a blinding hazard There is also a burn hazard at the li...

Page 46: ... If the microscope is equipped with an infinity port in addi tion to the rear output there is another access to the fluo rescence axis on the left right side of the stand The infinity port is equipped with a mirror or a prism Infinity port with mirror t 5IF JOmOJUZ QPSU TMJEF CBS PO UIF SJHIU MFGU TJEF PG UIF TUBOE guides 100 of the light to the rear output mirror swung out or 100 of the light to ...

Page 47: ...cence axis Leica DMi8 with motorized shutter t 0QFO PS DMPTF UIF TIVUUFS VTJOH UIF TIVUUFS LFZ PO UIF MFGU side of the stand This switches the active illumination axis TL or Fluor on or off When the shutter is open the LED is illuminated t MUFSOBUJWFMZ UIF TIVUUFS DBO CF PQFSBUFE VTJOH CVUUPOT on the Touch Screen Gray Shutter closed Yellow Shutter open Manual integrated fluorescence axis Manual ex...

Page 48: ...knurled disk During the manual objective change be sure that the nosepiece locks into place The LED assigned to the selected objective on the front side of the microscope is illuminated Leica DMi8 with motorized objective nosepiece and front operating panel with function keys t POmHVSF BO PCKFDUJWF CZ QSFTTJOH UIF DPSSFTQPOEJOH PC jective changer button on the keypad of the front side of the micro...

Page 49: ...icroscope t 5IF NPTU SFDFOUMZ DPOmHVSFE MJHIU JOUFOTJUZ JO UIF SFTQFD tive contrast method with DIC fine adjustment where ap plicable t 5IF NPTU SFDFOUMZ DPOmHVSFE GPDVT JODMVEJOH QBSGPDBM ity Adjusting the objectives using the Touch Screen t 6TF UIF tab to switch to the Magnification menu The information field on the top edge of the screen shows the current total magnification at the specified ob...

Page 50: ... dry objective to the immersion objective Conversely the immersion fluid can be re moved The current objective remains in the beam path t 1SFTT UIF LFZ GPS UIF EFTJSFE JNNFSTJPO PCKFDUJWF XIJDI is now approached Note For lockable immersion objectives press the front part upwards as far as it will go approx 2 mm Then after a gentle turning motion to the right the objective is locked For objectives ...

Page 51: ...JTUBODF PG UIF FZFQJFDF UVCFT TP UIBU a congruent total image is seen Adjusting the viewing angle t PS UIF FSHP UVCFT UIF WJFXJOH BOHMF DBO CF BEKVTUFE from 30 to 45 by tilting the binocular eyepiece Beam splitting in photo tubes The light division is set manually by pulling out a control bar Observation VIS Photo 100 0 0 100 Alternatively 50 50 BL Activating the Bertrand lens Switch rod Bertrand ...

Page 52: ...FZF BOE GPDVT PO UIF PCKFDU CZ BEKVTUJOH UIF second ocular only Correction for vision problems t 8JUI ZPVS SJHIU FZF MPPL UISPVHI UIF SJHIU FZFQJFDF BOE bring the specimen into sharp focus t 5IFO XJUI ZPVS MFGU FZF WJFX UIF TBNF QPTJUJPO PG UIF specimen and rotate the left eyepiece tube until this posi tion is brought into sharp focus While doing so do not change the adjustment of the z position N...

Page 53: ...t is carried out manually by moving the specimen on the specimen stage or using the object guide Manual 3 plate cross stage The object displacement in the x and y direction takes place using a coaxial drive Motorized 3 plate cross stage Object displacement in the x and y direction can be per formed by the Leica SmartMove control element the Leica STP8000 main switch board or LAS X software Scannin...

Page 54: ...ed of the stage by switching between the Coarse and Fine step sizes If the Coarse value is selected the travel speed is the same for all objectives The Fine value is adapted to the respective objective 5 6 3 Saving and moving to stage positions In the LAS X software the programmed working area is marked by a dashed line frame You can reset the working area to the maximum stage travel range via the...

Page 55: ...rwritten t PWJOH UP UIF DVSSFOU TUPSFE QPTJUJPO VTJOH UIF key When driving to the load position the objective nose piece is raised to the lower threshold Note If a stored position is outside the travel range of the stage for example as a result of subsequent changes to the travel range in the LAS software you must restore the maximum range of the stage via Limits to reach the position The stored p...

Page 56: ...NBHF CZ UVSOJOH UIF GPDVT EJBMT PO UIF left and right sides of the stand Coarse focusing Fine focusing Focusing Leica DMi8 with motorized focus closed loop t PDVT UIF JNBHF VTJOH UIF SFBS SPUBSZ LOPC PO UIF Leica SmartMove control element t MUFSOBUJWFMZ UIF focus dials on the left and right sides of the stand can also be used Note For coarse and fine focusing with the handwheel 2 focus stages each...

Page 57: ...ing the parfocality before setting the thresholds and if necessary teaching them in again using the LAS X software Note The focus position and stops are stored by the microscope and retained from one session to the next when power is switched off Setting thresholds Lower focus threshold t 1SFTT UIF SET key and the key p Z When the threshold is set the LED is illuminated Pressing the same key combi...

Page 58: ...PTJUJPO PS UIF MPXFS threshold select the corresponding position under Set Delete then press the Set or Delete key Focus position Lower stop 5 7 3 Adjusting the step sizes Leica DMi8 with motorized focus closed loop t 6TF Coarse and Fine to switch between coarse and fine focusing at the stand handwheel or the focus knob of the Leica SmartMove as applicable The Fine value is adapted to the respecti...

Page 59: ...ed then the specimen on the stand is focused using the handwheel and the stopping position is saved t NPOJUPSJOH DBO CF QFSGPSNFE VTJOH WBSJBCMF GVODUJPO keys LAS X software or via the Touch Screen Setting the AFC via the Touch Screen t 0O UIF 5PVDI 4DSFFO VTF UIF key for switching to stage and focus controls t 4FMFDU UIF Adaptive Focus Control menu using the key t DUJWBUF UIF VTJOH UIF On Off key...

Page 60: ... 20 80 Alternatively 0 100 Leica DMi8 with motorized ports Setting the ports via the Touch Screen t 6TF UIF key to switch to the Magnification menu t 4FMFDU UIF SJHIU PS MFGU TJEF QPSU VTJOH UIF DPSSFTQPOEJOH key or point 100 of the light at the eyepiece Left side port Right side port 100 VIS Bottom port Note The Select the bottom port function can be assigned to one of the variable function keys ...

Page 61: ...e top port Leica DMi8 with motorized Magnification Changer As an option a motorized Magnification Changer with 2 or 3 switch positions can be used Magnification factors For 3 switch positions 1x 1 6x 2x For 2 switch positions 1x 1 6x Setting the Magnification Changer via the Touch Screen t 6TF UIF key to switch to the Magnification menu t 4FMFDU UIF NBHOJmDBUJPO GBDUPS VTJOH UIF DPSSFTQPOEJOH key ...

Page 62: ...m Leica DMi8 with manual illuminated field diaphragm For a tiltable Transmitted Light arm t 5IF NBOVBM JMMVNJOBUFE mFME EJBQISBHN JT BEKVTUFE PO UIF arm Note The illuminated field diaphragm is available only in conjunc tion with S28 condensers For an integrated fluorescence axis t 5IF NBOVBM JMMVNJOBUFE mFME EJBQISBHN JT BEKVTUFE VTJOH a slide bar on the right side of the microscope Leica DMi8 wit...

Page 63: ...aphragm keys the motorized diaphragms can be changed at any time The display on the Touch Screen changes accordingly Note The old values will be overwritten by the current ones Note When using PH or DF the aperture diaphragm is open all the way and cannot be closed Setting the diaphragms via the Touch Screen t 0O UIF 5PVDI 4DSFFO VTF UIF key for basic micro scope settings t 4FMFDU UIF Status Illum...

Page 64: ...e Transmitted Light arm Note The front position is assigned at the factory using a shutter dark stop which is interchangeable with a filter Leica DMi8 with tiltable Transmitted Light arm Transmitted Light filter Leica DMi8 with a fixed Transmitted Light arm Transmitted Light filter For a fixed Transmitted Light arm t OTFSU UIF mMUFS JOUP UIF mMUFS SFDFQUBDMF PO UIF 5SBOTNJU ted Light arm 62 ...

Page 65: ...OH B NPUPSJ FE nVPSFTDFODF UVSSFU EJTL XJUI FJDB Radio Frequency Identification Device there is an auto matic full rotation of the turret to read out the filter For additional instructions see the chapter entitled Leica RFID Module o S 65 Leica DMi8 with manually encoded fluorescent turret disk t 5IF mMUFS DVCFT BSF TXJWFMFE NBOVBMMZ JOUP UIF CFBN QBUI by turning the fluorescent turret disk The LE...

Page 66: ... with lasers Leica Microsystems CMS GmbH offers special microscope variants with additional safety features Laser couplings require corresponding safety devices that have to be in spected and installed by trained personnel t 5IF cover of the fluorescent turret disk is secured using 2 DIN 912 M4x6 lens head Allen screws Order No 11 703 121 630 000 Detach both screws WARNING Store the screws in a sa...

Page 67: ...C Rules These limits are designed to provide rea sonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequen cy energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a res idential a...

Page 68: ...ieces for a short time and can temporarily blind the user Leica DMi8 with manual Transmitted Light method The contrast method on the manual or encoded Leica DMi8 can be operated using the manual condenser the manual objective nosepiece or the rotary knobs and slide bars on the stand t G OFDFTTBSZ mSTU UPHHMF UP UIF Transmitted Light axis TL by opening the shutter Slide bar on the manual external o...

Page 69: ...ht arm t PS DPSSFDU IFJHIU BEKVTUNFOU PG UIF 4 BOE S40 0 45 condensers the stand has marks 1 These marks pertain to a liquid height of 15 mm For stands with double marks the upper and lower mark repre sent a range with different liquid heights Move the condenser holder while observing the specimen to at tain an optimal image Note For the stands with 3 plate mechanical stages posi tion the arm 25 m...

Page 70: ...ret disk is approached automatically t OTFSU B 5SBOTNJUUFE JHIU TQFDJNFO t 4FMFDU B TVJUBCMF PCKFDUJWF JO UIF NBHOJmDBUJPO NFOV t PDVT UIF JNBHF VTJOH UIF SPUBSZ LOPC PO UIF FJDB SmartMove or using the focus dial t POmHVSF UIF CSJHIUOFTT PO UIF JOUFOTJUZ SPUBSZ LOPC Note You can find additional information regarding the correct settings for Koehler illumination in Leica Science Lab Link http www l...

Page 71: ...ft laterally into the re ceptacle until it clicks into place Caution there are various slide bars for the condens ers t 6TF UIF MJHIU SJOH PG UIF MJHIU SJOH TMJEF UIBU DPSSFTQPOET to the magnification 5 10 20 or 40 Note The Phase Contrast light ring slide is encoded As soon as the slider is taken out of the Brightfield position available opening and engaged in a Phase Contrast position any light r...

Page 72: ... Transmitted Light method t 4FMFDU UIF PH contrast method Phase Contrast The correct light ring on the motorized condenser is swung inwards t OTFSU B 5SBOTNJUUFE JHIU TQFDJNFO t 4FMFDU B TVJUBCMF PCKFDUJWF JO UIF NBHOJmDBUJPO NFOV Objectives that are suitable for Phase Contrast are en graved with PH t PDVT UIF JNBHF VTJOH UIF SPUBSZ LOPC PO UIF FJDB SmartMove or using the focus dial t POmHVSF UIF ...

Page 73: ...HIUOFTT PO UIF JOUFOTJUZ SPUBSZ LOPC Leica DMi8 with motorized Transmitted Light method t 4FMFDU UIF DPOUSBTU NFUIPE DF Darkfield The Darkfield ring dark stop is swung in for the motor ized condenser t OTFSU B 5SBOTNJUUFE JHIU TQFDJNFO t 4FMFDU B TVJUBCMF PCKFDUJWF JO UIF NBHOJmDBUJPO NFOV t PDVT UIF JNBHF VTJOH UIF SPUBSZ LOPC PO UIF FJDB SmartMove or using the focus dial t POmHVSF UIF CSJHIUOFTT...

Page 74: ...NT PVU PG UIF CFBN QBUI t 4FMFDU ZPVS PCKFDUJWF t 4XJOH UIF polarizer on the condenser into the beam path t OTFSU UIF analyzer on the right side of the stand up to the click stop t SJOH UIF QPMBSJ FS BOE BOBMZ FS JOUP DSPTT QPTJUJPO VOUJM they reach maximum darkness t OTFSU B TQFDJNFO t PDVT UIF JNBHF VTJOH UIF GPDVT EJBMT t POmHVSF UIF CSJHIUOFTT PO UIF JOUFOTJUZ SPUBSZ LOPC Condenser setting for...

Page 75: ...am path Combined procedure t PV BMTP IBWF UIF PQUJPO PG DPNCJOJOH QVSFMZ NFDIBOJDBM and motorized components i e you can combine a me chanical analyzer and a motorized polarizer Manual procedure For a manual swing polarizer and a mechanical analyzer proceed with the manual Transmitted Light method as de scribed for the Leica DMi8 t 4FMFDU B TVJUBCMF PCKFDUJWF JO UIF NBHOJmDBUJPO NFOV t OTFSU B TQF...

Page 76: ...IF DPSSFTQPOEJOH 8PMMBTUPO QSJTN PCKFDUJWF PO the objective nosepiece t 4XJOH UIF polarizer on the condenser into the beam path t 5IFO JOTFSU UIF analyzer on the right side of the stand un til it locks into place t OTFSU B TQFDJNFO t PDVT UIF JNBHF VTJOH UIF GPDVT EJBMT t POmHVSF UIF CSJHIUOFTT PO UIF JOUFOTJUZ SPUBSZ LOPC t PS mOF BEKVTUNFOU VTF UIF BEKVTUJOH TDSFX CFMPX UIF objective nosepiece S...

Page 77: ...JO UIF NBHOJmDBUJPO NFOV t PDVT UIF JNBHF VTJOH UIF SPUBSZ LOPC PO UIF FJDB SmartMove or using the focus dial t POmHVSF UIF CSJHIUOFTT PO UIF JOUFOTJUZ SPUBSZ LOPC t 5IF BEKVTUNFOU JT DBSSJFE PVU WJB UIF CVUUPOT and on the Touch Screen Manual alternative t BOVBMMZ TXJOH UIF QPMBSJ FS PO UIF DPOEFOTFS JOUP UIF beam path t 5IFO BMTP NBOVBMMZ JOTFSU UIF BOBMZ FS PO UIF SJHIU TJEF of the stand until i...

Page 78: ...rast module evenly from the front on the left side of the microscope t 4MJEF UIF 1IBTF POUSBTU NPEVMF JOUP UIF DPSSFDU FZFQPJOU B or C t OTFSU B 1IBTF POUSBTU TQFDJNFO t PDVT UIF JNBHF VTJOH UIF GPDVT EJBMT t POmHVSF UIF CSJHIUOFTT PO UIF JOUFOTJUZ SPUBSZ LOPC IP0 for 5x e g NPlan 5x Objective with eyepoint B IP1 for 10x e g NPlan 10 x Objectives with eyepoint B and for 20x e g NPlan L 20 x Object...

Page 79: ...NFOV eyepoint B or C t G OFDFTTBSZ SFNPWF UIF DPWFS PG UIF IPMEFS BOE JOTFSU the Phase Contrast module evenly from the front on the left side of the microscope t 4MJEF UIF 1IBTF POUSBTU NPEVMF JOUP UIF DPSSFDU FZFQPJOU B or C t PDVT UIF JNBHF VTJOH UIF SPUBSZ LOPC PO UIF FJDB SmartMove or using the focus dial Note When selecting the Phase Contrast method the aperture diaphragm is opened fully and ...

Page 80: ...croscope For a tiltable Transmitted Light arm t 4MJEF UIF NPEVMF JOUP UIF DPSSFDU FZFQPJOU PS For a fixed Transmitted Light arm t OTFSU UIF NPEVMBUPS JOUP UIF NPEVMF GSPN UIF side according to the eyepoint B C or D of the objec tive Ensure that the TOP marking faces upwards t OHBHF UIF TMJEF JO UIF QPTJUJPO Push in for IMC pull out for the Brightfield IMC module for fixed Transmitted Light arm 1 I...

Page 81: ...denser is used various IMC slit diaphragm sliders ex ist t OTFSU UIF EJBQISBHN TMJEF GSPN UIF SJHIU JOUP UIF Transmitted Light arm t OHBHF UIF TMJEF JO UIF QPTJUJPO 5IF SPVOE PQFO ing is the Brightfield position Note The IMC slit diaphragm slider is encoded As soon as the slider is taken out of the Brightfield position avail able opening and engaged in the IMC position the brightness increases Con...

Page 82: ...UP BE just the slit diaphragms to the focal plane This setting depends on the specimen being observed Note The position of the polarizer influences the appearance of the relief The best relief images are obtained in the extinc tion position the relief disappears at a 90 position For a fixed Transmitted Light arm IMC module for fixed Transmitted Light arm t 5IF fine adjustment occurs via the knurle...

Page 83: ...IF FJDB SmartMove or using the focus dial t POmHVSF UIF CSJHIUOFTT PO UIF JOUFOTJUZ SPUBSZ LOPC t 5IF fine adjustment is made using the knurled screw 5 and the polarizer Note The position of the polarizer influences the appearance of the relief The best relief images are obtained in the extinc tion position the relief disappears at a 90 position t 6TJOH UIF TMJEFS ZPV DBO BMTP PGGTFU UIF IFJHIU UP...

Page 84: ...ght source Note IftheLeicaDMi8isequippedwitha2portinfinityport o S 44 switch over to the current port o S 58 t 8IFO VTJOH UIF Fluorescence Intensity Manager FIM The brightness is adjusted in 5 defined increments on the FIM slider 4 100 55 30 17 10 t 5IF filter cubes are swiveled manually into the beam path by turning the fluorescent turret disk 1 t DPMPS NBSL PO UIF FEHF PG UIF EJTL JOEJDBUFT UIF ...

Page 85: ...ube changer button is illuminated Leica DMi8 with motorized fluorescence t G OFDFTTBSZ TXJUDI PWFS UP UIF nVPSFTDFODF BYJT Fluor with the button TL Fluor Operating the fluorescence via Touch Screen t G OFDFTTBSZ TXJUDI PWFS UP UIF nVPSFTDFODF BYJT MVPS with the IL button t 3PUBUF PO UIF 5PVDI 4DSFFO WJB UIF CVUUPO for con figuring the contrast method t PS JODJEFOU MJHIU TFMFDU UIF DPOUSBTU NFUIPE ...

Page 86: ... SPUBSZ LOPC PO UIF FJDB SmartMove or using the focus dial t 4FU UIF MJHIU JOUFOTJUZ PO UIF Leica EL6000 compact light source See separate Instructions for Use t 8IFO VTJOH UIF Fluorescence Intensity Manager FIM The brightness is adjusted in 5 defined increments on the brightness adjusting knob 100 55 30 17 10 or alternatively on the Touch Screen via the button FIM 84 ...

Page 87: ...FLUO PH t 5IF DVSSFOU nVPSFTDFODF mMUFS DVCF JT EJTQMBZFE PO UIF Touch Screen If necessary push the button for the de sired fluorescence filter cube t 5IF JMMVNJOBUJPO TFUUJOHT GPS UIF nVPSFTDFODF BOE 5SBOT mitted Light axis can be adjusted separately To do so toggle between the using the TL Fluor button FLUO DIC t 4XJUDI CFUXFFO UIF UXP DPOUSBTU NFUIPET CZ QSFTTJOH the FLUO DIC key t O 60 NPEF UI...

Page 88: ...ection of the lamp housing to the micro scope Transmitted Light Ensure that the lamps are not defective Check the connection of the EL6000 compact light source fluorescence Switch over to the active port Inform the service department and have them check whether a fuse is defective Remove all unneeded filters from the light path Use the correct immersion medium Place the specimen with the cover sli...

Page 89: ...is too weak Use a higher magnification If necessary remove the condenser lenses or the con denser head Clean the specimen and neighboring lens surfaces The specimen is too thick too thin or too brightly stained Refractive indexes of the mounting medium and specimen are identical so that there is no phase jump The cover glass is not placed uniformly Check that the correct light ring is positioned C...

Page 90: ...at the correct objective is positioned eyepoint C or D Check the position of the aperture diaphragm For a fixed Transmitted Light arm Check that the IMC modulator and the IMC slit diaphragm slider are inserted correctly and engaged in the IMC posi tion For a tiltable Transmitted Light arm Check that the IMC module is inserted correctly and en gaged in the position compatible to the objective Check...

Page 91: ...ion condensation may occur 7 2 Cleaning Caution Residual fiber and dust can create unwanted background fluorescence during fluorescence microscopy Cleaning coated parts Dust and loose dirt particles can be removed with a soft brush or lint free cotton cloth Clinging dirt can be cleaned as necessary with a low con centrated soap solution petroleum ether or ethyl alcohol For cleaning coated parts us...

Page 92: ...d micro scopes Select the microscope type and go to the Download page In case of questions please contact our Technical Service Removing immersion oil Caution Follow safety notes for immersion oil First wipe off the immersion oil with a clean cotton cloth and then re wipe the surface several times with ethyl alco hol 7 3 Handling acids and bases For examinations using acids or other aggressive che...

Page 93: ...up 10x 25 eyepiece 021 264 520 018 C PLAN eyecup 10x 22 eyepiece 021 264 520 018 HC PLAN eyecup 10x 20 eyepiece Immersion oil in accordance with DIN ISO standards fluorescence free 11 513 859 Type F ISO 8036 OIL and IMM objectives Very low self fluorescence and oil condenser heads Expressly recommended for fluorescence applications and all APO objectives 10 ml 11 513 860 Type N ISO 8036 Low self f...

Page 94: ...tom port if present Otherwise Port switchover Industry applications Microscope with Magnification Changer Button 1 TL Button 2 Photo Button 3 Toggle Magnification Changer Button 4 Toggle ports Button 1 Button 2 Button 3 Button 4 Biological applications Microscope without Magnification Changer Button 1 Switch through objectives to the right Button 2 Photo Button 3 Switch through objectives to the l...

Page 95: ... counterclockwise 6 o 1 CHG FLUO Switches through all fluorescence cubes INT FLUO n Increases the brightness fluorescence INT FLUO p Reduces the brightness fluorescence FD FLUO n Opens the field diaphragm fluorescence FD FLUO p Closes the field diaphragm fluorescence CHG FW Toggles the filter wheel functions IFW Activates the internal filter wheel SHT FL Opens closes the fluo shutter ExMan Activat...

Page 96: ...Field diaphragm FIM Fluorescence intensity manager FLUO Fluor Fluorescence axis incident light ICR Interference contrast incident light ICT Interference contrast Transmitted Light IFW Internal filter wheel IL Incident light INT Intensity IMC Integrated Modulation Contrast IPH Integrated Phase Contrast PH Phase Contrast POL Polarization incident Transmitted Light TL Transmitted Light SET Set focus ...

Page 97: ... immersion objective Shutter Incident light shutter open Transmitted light shutter closed Observation ports 100 eyepiece 100 camera Eyepiece camera splitting 100 to lower left or right port Top port eyepiece camera splitting Lower port left or right port split Left or right port split top port with further split ting Top port rear port split Control Toggle button Saving Delete For the table reset ...

Page 98: ...tion contrast Differential Interference Contrast t MVPSFTDFODF UFDIOJRVFT t 5IF JOTUSVDUJPOT GPS DMFBOJOH UIF NJDSPTDPQF PQUJDT and much more Objectives For information on the objectives such as the objective classes and the marking of objectives refer to http www leica microsystems com de produkte objektive Accessories The Live on Stage brochure provides an overview of the accessories for live ce...

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